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ATTO 488 azide

Product key features

  • Ex/Em: 499/520 nm
  • Extinction coefficient: 90,000 cm-1M-1
  • Reactive group: azide
  • Efficient Conjugation: Readily and selectively labels alkyne targets via click chemistry
  • High Quantum Yield & Photostability: Delivers consistent, intense fluorescence under demanding conditions
  • High Hydrophilicity: Efficient aqueous dispersion enhances signal clarity for advanced imaging and live-cell applications

Product description

ATTO 488 is a hydrophilic, rhodamine-based fluorescent dye with exceptional water solubility. It is characterized by strong absorption, a high fluorescence quantum yield, and exceptional photostability, making it highly suitable for advanced fluorescence imaging techniques. The dye exhibits optimal excitation within the 480-515 nm wavelength range, aligning precisely with the 488 nm emission line of the Argon-Ion laser. ATTO 488 is particularly effective for single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. Moreover, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other bioanalytical applications.

The azide derivative of ATTO 488 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.

Spectrum

References

View all 32 references: Citation Explorer
Time-resolved fluorescence anisotropy with Atto 488-labeled phytochrome Agp1 from Agrobacterium fabrum.
Authors: Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman
Journal: Photochemistry and photobiology (2024): 561-572
Analysis of photon trajectories from diffusing single molecules.
Authors: Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung
Journal: The Journal of chemical physics (2023)
Curvature preference of cubic CsPbBr3 quantum dots embedded onto phospholipid bilayer membranes.
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
Interactions of the Kv1.1 Channel with Peptide Pore Blockers: A Fluorescent Analysis on Mammalian Cells.
Authors: Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V
Journal: Membranes (2023)
Spatial distribution of single guest molecules along thickness of thin films of poly(2-hydroxyethyl acrylate).
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184
Page updated on April 25, 2025

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Catalog Number2817
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Physical properties

Molecular weight

771.70

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.22

Correction Factor (280 nm)

0.09

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

499

Emission (nm)

520

Quantum yield

0.80

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Schematic illustrating the azide–alkyne cycloaddition (“click chemistry”) between an azide‐functionalized dye and an alkyne‐presenting biomolecule. In the presence of a copper catalyst (CuAAC) or under strain‐promoted conditions (SPAAC), the azide and alkyne react to form a stable 1,2,3‐triazole linkage. This highly selective and robust reaction proceeds under mild conditions and tolerates a wide range of functional groups.
Schematic illustrating the azide–alkyne cycloaddition (“click chemistry”) between an azide‐functionalized dye and an alkyne‐presenting biomolecule. In the presence of a copper catalyst (CuAAC) or under strain‐promoted conditions (SPAAC), the azide and alkyne react to form a stable 1,2,3‐triazole linkage. This highly selective and robust reaction proceeds under mild conditions and tolerates a wide range of functional groups.
Schematic illustrating the azide–alkyne cycloaddition (“click chemistry”) between an azide‐functionalized dye and an alkyne‐presenting biomolecule. In the presence of a copper catalyst (CuAAC) or under strain‐promoted conditions (SPAAC), the azide and alkyne react to form a stable 1,2,3‐triazole linkage. This highly selective and robust reaction proceeds under mild conditions and tolerates a wide range of functional groups.
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