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ATTO 488 Tetrazine

Product key features

  • Ex/Em: 499/520 nm
  • Extinction coefficient: 90,000 cm-1M-1
  • Reactive group: Tetrazine
  • Click Chemistry Conjugation: Provides rapid and copper-free labeling of TCO-modified biomolecules
  • Superior Brightness & Stability: Delivers robust, steady fluorescence for sensitive applications
  • High Hydrophilicity: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Product description

ATTO 488 is a hydrophilic, rhodamine-based fluorescent dye with exceptional water solubility. It is characterized by strong absorption, a high fluorescence quantum yield, and exceptional photostability, making it highly suitable for advanced fluorescence imaging techniques. The dye exhibits optimal excitation within the 480-515 nm wavelength range, aligning precisely with the 488 nm emission line of the Argon-Ion laser. ATTO 488 is particularly effective for single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. Moreover, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other bioanalytical applications.

ATTO 488 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

 

Spectrum

References

View all 32 references: Citation Explorer
Time-resolved fluorescence anisotropy with Atto 488-labeled phytochrome Agp1 from Agrobacterium fabrum.
Authors: Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman
Journal: Photochemistry and photobiology (2024): 561-572
Analysis of photon trajectories from diffusing single molecules.
Authors: Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung
Journal: The Journal of chemical physics (2023)
Curvature preference of cubic CsPbBr3 quantum dots embedded onto phospholipid bilayer membranes.
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
Interactions of the Kv1.1 Channel with Peptide Pore Blockers: A Fluorescent Analysis on Mammalian Cells.
Authors: Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V
Journal: Membranes (2023)
Spatial distribution of single guest molecules along thickness of thin films of poly(2-hydroxyethyl acrylate).
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184
Page updated on April 25, 2025

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Catalog Number2800
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Physical properties

Molecular weight

874.00

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.22

Correction Factor (280 nm)

0.09

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

499

Emission (nm)

520

Quantum yield

0.80

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Product Image
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Gallery Image 1
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a tetrazine–dye conjugate and a trans‐cyclooctene (TCO)–modified biomolecule. The tetrazine–dye selectively and rapidly reacts with the TCO moiety on the biomolecule to form a stable linkage, labeling the nucleic acid with the dye. This bioorthogonal “click chemistry” process is fast, selective, and well‐suited for biocompatible labeling applications.