ATTO 488 Tetrazine
Product key features
- Ex/Em: 499/520 nm
- Extinction coefficient: 90,000 cm-1M-1
- Reactive group: Tetrazine
- Click Chemistry Conjugation: Provides rapid and copper-free labeling of TCO-modified biomolecules
- Superior Brightness & Stability: Delivers robust, steady fluorescence for sensitive applications
- High Hydrophilicity: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications
Product description
ATTO 488 is a hydrophilic, rhodamine-based fluorescent dye with exceptional water solubility. It is characterized by strong absorption, a high fluorescence quantum yield, and exceptional photostability, making it highly suitable for advanced fluorescence imaging techniques. The dye exhibits optimal excitation within the 480-515 nm wavelength range, aligning precisely with the 488 nm emission line of the Argon-Ion laser. ATTO 488 is particularly effective for single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. Moreover, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other bioanalytical applications.
ATTO 488 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.
Spectrum
References
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Journal: The Journal of chemical physics (2023)
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
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Journal: Membranes (2023)
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Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184