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ATTO 532 Tetrazine

Product key features

  • Ex/Em: 531/552 nm
  • Extinction coefficient: 115,000 cm-1M-1
  • Reactive group: Tetrazine
  • Click Chemistry: enables copper-free, bioorthogonal labeling of TCO-functionalized biomolecules
  • High Quantum Yield & Photostability: ensures intense signal for reliable detection and imaging
  • Excellent Hydrophilicity: Prevents aggregation and enhances signal clarity in live-cell applications
  • Advanced Imaging Performance: Suitable for single-molecule detection and super-resolution techniques, including SIM and STED

Product description

ATTO 532 is a rhodamine-based fluorescent dye notable for its high molar absorptivity and fluorescence quantum yield (0.90), providing robust signal intensity in fluorescence applications. Its combination of photostability, aqueous solubility, and sufficient Stokes shift makes it suitable for single-molecule detection and high-resolution microscopy techniques, including SIM and STED microscopy. ATTO 532 is also effective in flow cytometry, FISH, and a variety of biological assays, offering flexibility for diverse fluorescence-based experimental protocols. The dye is optimally excited within the 515-545 nm range, with a frequency-doubled Nd:YAG laser at 532 nm serving as an ideal excitation source.

ATTO 532 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ATTO 532 acid5315521150000.900.220.11
ATTO 532 maleimide5315521150000.900.220.11
ATTO 390 Tetrazine39047524000.900.460.09
ATTO 425 Tetrazine438484450000.900.190.17
ATTO 495 Tetrazine497525800000.20.450.37
ATTO 550 Tetrazine5535741200000.800.230.10
ATTO 590 Tetrazine5926211200000.800.390.43
ATTO 610 Tetrazine6156321500000.700.030.06
ATTO 620 Tetrazine61964112000010.510.040.06
ATTO 633 Tetrazine6296511300000.6410.040.05
ATTO 655 Tetrazine6616791250000.310.240.08
ATTO 488 Tetrazine499520900000.800.220.09
ATTO 647N Tetrazine6456631500000.6510.060.05
ATTO 680 Tetrazine6796961250000.300.300.17
ATTO 700 Tetrazine6997151200000.250.260.41
ATTO 647 Tetrazine6466661200000.200.080.04
ATTO 594 Tetrazine6026211200000.850.260.51
ATTO 532 alkyne5315521150000.900.220.11
ATTO 532 TCO5315521150000.900.220.11
Show More (10)

References

View all 4 references: Citation Explorer
Evidence for Nanostructures of at Least 20 nm in a Phosphonium Ionic Liquid at Room Temperature Using Fluorescence Correlation Spectroscopy.
Authors: Maddala, Bala Gopal and Mahato, Jaladhar and Morgan, Ian T and Larson, Seth A and Brickley, Jayme A and Anderson, Jared L and Smith, Emily A and Song, Xueyu and Petrich, Jacob W
Journal: The journal of physical chemistry. B (2024)
Closing the green gap of photosystem I with synthetic fluorophores for enhanced photocurrent generation in photobiocathodes.
Authors: Morlock, Sascha and Subramanian, Senthil K and Zouni, Athina and Lisdat, Fred
Journal: Chemical science (2023): 1696-1708
Electrokinetic transport properties of deoxynucleotide monophosphates (dNMPs) through thermoplastic nanochannels.
Authors: O'Neil, Colleen and Amarasekara, Charuni A and Weerakoon-Ratnayake, Kumuditha M and Gross, Bethany and Jia, Zheng and Singh, Varshni and Park, Sunggook and Soper, Steven A
Journal: Analytica chimica acta (2018): 67-75
The key role of geminate electron-hole pair recombination in the delayed fluorescence in rhodamine 6G and ATTO-532.
Authors: Aydemir, Murat and Jankus, Vygintas and Dias, Fernando B and Monkman, Andy
Journal: Physical chemistry chemical physics : PCCP (2014): 21543-9
Page updated on December 6, 2024

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Catalog Number2869
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Physical properties

Molecular weight

930.11

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.22

Correction Factor (280 nm)

0.11

Extinction coefficient (cm -1 M -1)

115000

Excitation (nm)

531

Emission (nm)

552

Quantum yield

0.90

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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