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ATTO 495 Tetrazine

Product key features

  • Ex/Em: 497/525 nm
  • Extinction coefficient: 80,000 cm-1M-1
  • Reactive group: Tetrazine
  • Click Chemistry Conjugation: Provides rapid and copper-free labeling of TCO-modified biomolecules with high yield
  • Superior Brightness & Stability: Delivers intense fluorescence with high photostability and thermal stability
  • Long-Lived Phosphorescence: Enables sustained emission in solid matrices or at low temperatures, ideal for time-resolved spectroscopy

Product description

ATTO 495 is a green fluorescent dye derived from acridine orange, known for its strong absorption, high fluorescence quantum yield, excellent photostability, and superior thermal stability. It exhibits moderate hydrophilicity and is highly soluble in polar solvents such as DMF and DMSO, with an optimal excitation range of 465-510 nm. Notably, ATTO 495 exhibits intense and long-lived phosphorescence in solid matrices or at low temperatures. This dye is well-suited for advanced applications in single-molecule detection and high-resolution microscopy techniques, such as PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a wide range of other biological assays.

ATTO 495 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

References

View all 2 references: Citation Explorer
Generation and Characterization of Virus-Enhancing Peptide Nanofibrils Functionalized with Fluorescent Labels.
Authors: Rode, Sascha and Hayn, Manuel and Röcker, Annika and Sieste, Stefanie and Lamla, Markus and Markx, Daniel and Meier, Christoph and Kirchhoff, Frank and Walther, Paul and Fändrich, Marcus and Weil, Tanja and Münch, Jan
Journal: Bioconjugate chemistry (2017): 1260-1270
Utilization of magnetic nanobeads for analyzing haptoglobin in human plasma as a marker of Alzheimer's disease by capillary electrophoretic immunoassay with laser-induced fluorescence detection.
Authors: Wang, Yi-Rou and Yang, Yuan-Han and Lu, Chi-Yu and Chen, Su-Hwei
Journal: Analytica chimica acta (2015): 76-82
Page updated on April 22, 2025

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Catalog Number70227
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Physical properties

Molecular weight

635.12

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.45

Correction Factor (280 nm)

0.37

Extinction coefficient (cm -1 M -1)

80000

Excitation (nm)

497

Emission (nm)

525

Quantum yield

0.2

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Product Image
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Gallery Image 1
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a tetrazine–dye conjugate and a trans‐cyclooctene (TCO)–modified biomolecule. The tetrazine–dye selectively and rapidly reacts with the TCO moiety on the biomolecule to form a stable linkage, labeling the nucleic acid with the dye. This bioorthogonal “click chemistry” process is fast, selective, and well‐suited for biocompatible labeling applications.