logo
AAT Bioquest

ATTO 647N Tetrazine

Product key features

  • Ex/Em: 645/663 nm
  • Extinction coefficient: 150,000 cm-1M-1
  • Reactive group: Tetrazine
  • Click Chemistry Conjugation: Provides rapid, copper-free labeling of TCO-modified biomolecules
  • High Quantum Yield & Stability: Provides bright fluorescence with high photostability, thermal resilience, and pH insensitivity across 2–11
  • Ozone-Resistant: Enhanced resistance to atmospheric ozone degradation compared to cyanine dyes

Product description

ATTO 647N is a rhodamine-based fluorescent dye optimized for use in the red spectral region, with similar spectral characteristics as Cy5. It is characterized by high molar absorptivity, a strong fluorescence quantum yield, and excellent thermal and photostability. The dye is moderately hydrophilic and exhibits optimal excitation within the 625-660 nm range, making it compatible with the 647 nm line of Krypton-Ion lasers and the 650 nm line of diode lasers. ATTO 647N maintains stable fluorescence across a broad pH range (pH 2-11), supporting its application under diverse experimental conditions. Upon conjugation to a substrate, the dye becomes cationic, carrying a net positive charge of +1. Unlike cyanine dyes, ATTO 647N demonstrates enhanced resistance to atmospheric ozone degradation, which increases its reliability in microarray applications. ATTO 647N is particularly effective for high-precision applications such as single-molecule detection, super-resolution microscopy techniques (e.g., SIM and STED), flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

ATTO 647N tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

References

View all 7 references: Citation Explorer
Temperature-Dependent Constrained Diffusion of Micro-Confined Alkylimidazolium Chloride Ionic Liquids.
Authors: Stephens, Nicole M and Masching, Hayley P and Walid, Mohammad K I and Petrich, Jacob W and Anderson, Jared L and Smith, Emily A
Journal: The journal of physical chemistry. B (2022)
High-speed single molecule imaging datasets of membrane proteins in rat basophilic leukemia cells.
Authors: Mazloom-Farsibaf, Hanieh and Kanagy, William K and Lidke, Diane S and Lidke, Keith A
Journal: Data in brief (2020): 105424
Real-time 3D single molecule tracking.
Authors: Hou, Shangguo and Exell, Jack and Welsher, Kevin
Journal: Nature communications (2020): 3607
In vitro selection of tacrolimus binding aptamer by systematic evolution of ligands by exponential enrichment method for the development of a fluorescent aptasensor for sensitive detection of tacrolimus.
Authors: Mansouri, Atena and Abnous, Khalil and Nabavinia, Maryam Sadat and Ramezani, Mohammad and Taghdisi, Seyed Mohammad
Journal: Journal of pharmaceutical and biomedical analysis (2020): 112853
Conventional fluorescence microscopy below the diffraction limit with simultaneous capture of two fluorophores in DNA origami.
Authors: Glasgow, Ben J
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2016)
Page updated on April 22, 2025

Ordering information

Price
Unit size
Catalog Number2839
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

929.56

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.06

Correction Factor (280 nm)

0.05

Extinction coefficient (cm -1 M -1)

150000

Excitation (nm)

645

Emission (nm)

663

Quantum yield

0.651

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Product Image
Product Image
Gallery Image 1
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a tetrazine–dye conjugate and a trans‐cyclooctene (TCO)–modified biomolecule. The tetrazine–dye selectively and rapidly reacts with the TCO moiety on the biomolecule to form a stable linkage, labeling the nucleic acid with the dye. This bioorthogonal “click chemistry” process is fast, selective, and well‐suited for biocompatible labeling applications.