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ATTO 610 Tetrazine

Product key features

  • Ex/Em: 615/632 nm
  • Extinction coefficient: 150,000 cm-1M-1
  • Reactive group: Tetrazine
  • Rapid Conjugation: Ideal for labeling TCO-modified biomolecules under copper-free conditions, providing fast reaction kinetics
  • High Quantum Yield & Stability: Offers bright, stable fluorescence with excellent photostability and thermal stability for sensitive imaging applications
  • Ideal for Super-Resolution Imaging: Suited for PALM, dSTORM, and STED techniques in high-resolution microscopy

Product description

ATTO 610 is a carbopyronin-based fluorescent dye known for its strong absorption, high fluorescence quantum yield, and exceptional photostability and thermal stability. It is moderately hydrophilic and optimally excited at wavelengths between 595 and 625 nm. Upon coupling to a substrate, ATTO 610 becomes cationic, carrying a net electrical charge of +1. The dye remains stable under physiological pH conditions and in buffers with a pH of up to 8, though it gradually degrades at higher pH levels. ATTO 610 is ideal for advanced applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays.

ATTO 610 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ATTO 390 Tetrazine39047524000.900.460.09
ATTO 425 Tetrazine438484450000.900.190.17
ATTO 495 Tetrazine497525800000.20.450.37
ATTO 550 Tetrazine5535741200000.800.230.10
ATTO 590 Tetrazine5926211200000.800.390.43
ATTO 610 acid6156321500000.700.030.06
ATTO 610 maleimide6156321500000.700.030.06
ATTO 610 azide6156321500000.700.030.06
ATTO 610 alkyne6156321500000.700.030.06
ATTO 610 DBCO6156321500000.700.030.06
ATTO 610 TCO6156321500000.700.030.06
ATTO 620 Tetrazine61964112000010.510.040.06
ATTO 633 Tetrazine6296511300000.6410.040.05
ATTO 655 Tetrazine6616791250000.310.240.08
ATTO 488 Tetrazine499520900000.800.220.09
ATTO 647N Tetrazine6456631500000.6510.060.05
ATTO 680 Tetrazine6796961250000.300.300.17
ATTO 700 Tetrazine6997151200000.250.260.41
ATTO 647 Tetrazine6466661200000.200.080.04
ATTO 594 Tetrazine6026211200000.850.260.51
ATTO 532 Tetrazine5315521150000.900.220.11
Show More (12)

References

View all 4 references: Citation Explorer
Two-Photon-Excited Single-Molecule Fluorescence Enhanced by Gold Nanorod Dimers.
Authors: Lu, Xuxing and Punj, Deep and Orrit, Michel
Journal: Nano letters (2022): 4215-4222
Ultrabright Terbium Nanoparticles for FRET Biosensing and in Situ Imaging of Epidermal Growth Factor Receptors*.
Authors: Charpentier, Cyrille and Cifliku, Vjona and Goetz, Joan and Nonat, Aline and Cheignon, Clémence and Cardoso Dos Santos, Marcelina and Francés-Soriano, Laura and Wong, Ka-Leung and Charbonnière, Loïc J and Hildebrandt, Niko
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 14602-14611
Oxazine dye-conjugated dna oligonucleotides: Förster resonance energy transfer in view of molecular dye-DNA interactions.
Authors: Kupstat, Annette and Ritschel, Thomas and Kumke, Michael U
Journal: Bioconjugate chemistry (2011): 2546-57
Novel wavelength-resolved fluorescence detection for a high-throughput capillary electrophoresis system under a diascopic configuration.
Authors: Lin, Shi-Wei and Chang, Guan-Liang and Lin, Che-Hsin
Journal: Journal of chromatography. A (2008): 198-201
Page updated on December 6, 2024

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Catalog Number70257
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Physical properties

Molecular weight

687.77

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.03

Correction Factor (280 nm)

0.06

Extinction coefficient (cm -1 M -1)

150000

Excitation (nm)

615

Emission (nm)

632

Quantum yield

0.70

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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