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ATTO 680 Tetrazine

Product key features

  • Ex/Em: 679/696 nm
  • Extinction coefficient: 125,000 cm-1M-1
  • Reactive group: Tetrazine
  • Click Chemistry Conjugation: Offers high yield and rapid, copper-free labeling of TCO-modified biomolecules
  • High Quantum Yield & Stability: Provides bright fluorescence with high photostability and thermal resilience
  • Zwitterionic: Minimizes non-specific binding to biological surfaces, reducing background noise in live-cell imaging and super-resolution microscopy

Product description

ATTO 680 is a far-red fluorescent dye characterized by its strong absorption, high photo and thermal stability, and good aqueous solubility. It is optimally excited within the 645-695 nm wavelength range, which aligns with the 670 nm line of diode laser and the 676 nm line of Krypton-Ion laser. As a zwitterionic compound, ATTO 680 remains electrically neutral when conjugated to biomolecules or other substrates. Its strong electron-accepting properties result in efficient fluorescence quenching by electron donors such as guanine and tryptophan. These properties make ATTO 680 ideal for precise applications including single-molecule detection and super-resolution microscopy techniques like PALM, dSTORM, and STED. Furthermore, ATTO 680 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research.

ATTO 680 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

References

View all 3 references: Citation Explorer
Avidin/Biotin Bioinspired Platform for Dual In Vivo 18F-PET/NIRF Molecular Imaging.
Authors: Damont, Annelaure and Boisgard, Raphael and Dollé, Frédéric and Hollocou, Morgane and Kuhnast, Bertrand
Journal: Bioconjugate chemistry (2017): 2524-2529
Oxazine dye-conjugated dna oligonucleotides: Förster resonance energy transfer in view of molecular dye-DNA interactions.
Authors: Kupstat, Annette and Ritschel, Thomas and Kumke, Michael U
Journal: Bioconjugate chemistry (2011): 2546-57
Inter- and intramolecular fluorescence quenching of organic dyes by tryptophan.
Authors: Marmé, Nicole and Knemeyer, Jens-Peter and Sauer, Markus and Wolfrum, Jürgen
Journal: Bioconjugate chemistry (2003): 1133-9
Page updated on April 15, 2025

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Catalog Number70297
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Physical properties

Molecular weight

822.86

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.30

Correction Factor (280 nm)

0.17

Extinction coefficient (cm -1 M -1)

125000

Excitation (nm)

679

Emission (nm)

696

Quantum yield

0.30

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Product Image
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Gallery Image 1
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a tetrazine–dye conjugate and a trans‐cyclooctene (TCO)–modified biomolecule. The tetrazine–dye selectively and rapidly reacts with the TCO moiety on the biomolecule to form a stable linkage, labeling the nucleic acid with the dye. This bioorthogonal “click chemistry” process is fast, selective, and well‐suited for biocompatible labeling applications.