ATTO 514 Tetrazine

• Ex/Em: 510/531 nm
• Extinction coefficient: 115,000 cm^-1M^-1
• Reactive group: Tetrazine
• Click Chemistry Conjugation: Offers high yield and rapid, copper-free labeling of TCO-modified biomolecules
• Superior Brightness & Stability: Provides robust quantum yield with high photostability and thermal stability
• Excellent Hydrophilicity: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

ATTO 514 Tetrazine is manufactured by AAT Bioquest for research and development use. ATTO 514 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions, with the bright, green fluorescent ATTO 514 dye. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

ATTO 514 is a hydrophilic fluorescent dye characterized by excellent aqueous solubility, strong molar absorptivity, high fluorescence quantum yield, and notable thermal and photostability. These properties make it well-suited for advanced imaging techniques, including single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. ATTO 514 is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other fluorescence-based assays. The dye is optimally excited within the 510–535 nm range, with the 514 nm line of an Argon-Ion laser providing efficient excitation.