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ATTO 514 acid
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 753.64 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.21 |
| Correction Factor (280 nm) | 0.08 |
| Extinction coefficient (cm -1 M -1) | 115,000 |
| Excitation (nm) | 510 |
| Emission (nm) | 531 |
| Quantum yield | 0.85 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
Alternative formats
| ATTO 514 maleimide |
| Overview |  SDSProtocol |
Molecular weight 753.64 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.08 | Extinction coefficient (cm -1 M -1) 115,000 | Excitation (nm) 510 | Emission (nm) 531 | Quantum yield 0.85 |
ATTO 514 is a rhodamine-based fluorophore characterized by exceptional water solubility, strong absorption, high fluorescence quantum yield, and outstanding thermal and photo-stability. This makes it highly suitable for applications in single-molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED. Additionally, it finds utility in flow cytometry (FACS), fluorescence in-situ hybridization (FISH), and various scientific methodologies. ATTO 514 is hydrophilic and exhibits optimal fluorescence efficiency when excited within the 510-535 nm range, making it an ideal match for the 514 nm line of an Argon-Ion laser.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ATTO 514 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 132.689 µL | 663.447 µL | 1.327 mL | 6.634 mL | 13.269 mL |
| 5 mM | 26.538 µL | 132.689 µL | 265.379 µL | 1.327 mL | 2.654 mL |
| 10 mM | 13.269 µL | 66.345 µL | 132.689 µL | 663.447 µL | 1.327 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.21 |
| Correction Factor (280 nm) | 0.08 |
| Extinction coefficient (cm -1 M -1) | 115,000 |
| Excitation (nm) | 510 |
| Emission (nm) | 531 |
| Quantum yield | 0.85 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| ATTO 488 acid | 499 | 520 | 90000 | 0.80 | 0.25 | 0.10 |
| ATTO 532 acid | 531 | 552 | 115000 | 0.90 | 0.22 | 0.11 |
| ATTO 647 acid | 646 | 666 | 120000 | 0.20 | 0.08 | 0.04 |
| ATTO 647N acid | 645 | 663 | 150000 | 0.651 | 0.06 | 0.05 |
| ATTO 594 acid | 602 | 621 | 120000 | 0.85 | 0.26 | 0.51 |
Images
Figure 1. Chemical structure for ATTO 514 acid.
References
View all 7 references: Citation Explorer
The new live imagers MitoMM1/2 for mitochondrial visualization.
Authors: Maeda, Miwa and Suzuki, Mayu and Takashima, Shigeo and Sasaki, Tsutomu and Oh-Hashi, Kentaro and Takemori, Hiroshi
Journal: Biochemical and biophysical research communications (2021): 50-54
Authors: Maeda, Miwa and Suzuki, Mayu and Takashima, Shigeo and Sasaki, Tsutomu and Oh-Hashi, Kentaro and Takemori, Hiroshi
Journal: Biochemical and biophysical research communications (2021): 50-54
DNA-templated control of chirality and efficient energy transport in supramolecular DNA architectures with aggregation-induced emission.
Authors: Ucar, Hülya and Wagenknecht, Hans-Achim
Journal: Chemical science (2021): 10048-10053
Authors: Ucar, Hülya and Wagenknecht, Hans-Achim
Journal: Chemical science (2021): 10048-10053
Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter.
Authors: Mathiassen, Patricia P M and Menon, Anant K and Pomorski, Thomas Günther
Journal: Scientific reports (2021): 14364
Authors: Mathiassen, Patricia P M and Menon, Anant K and Pomorski, Thomas Günther
Journal: Scientific reports (2021): 14364
Importance of probe design for bioanalysis of oligonucleotides using hybridization-based LC-fluorescence assays.
Authors: Ji, Yuhuan and Liu, Yijiang and Xia, Wanhong and Behling, Alexander and Meng, Min and Bennett, Patrick and Wang, Laixin
Journal: Bioanalysis (2019): 1917-1925
Authors: Ji, Yuhuan and Liu, Yijiang and Xia, Wanhong and Behling, Alexander and Meng, Min and Bennett, Patrick and Wang, Laixin
Journal: Bioanalysis (2019): 1917-1925
The effect of local dynamics of Atto 390-labeled lysozyme on fluorescence anisotropy modeling.
Authors: Babcock, Jeremiah J and Brancaleon, Lorenzo
Journal: Biopolymers (2015): 285-95
Authors: Babcock, Jeremiah J and Brancaleon, Lorenzo
Journal: Biopolymers (2015): 285-95
Binding assay for low molecular weight analytes based on reflectometry of absorbing molecules in porous substrates.
Authors: Stephan, Milena and Kramer, Corinna and Steinem, Claudia and Janshoff, Andreas
Journal: The Analyst (2014): 1987-92
Authors: Stephan, Milena and Kramer, Corinna and Steinem, Claudia and Janshoff, Andreas
Journal: The Analyst (2014): 1987-92
Fluorescently labeled substrates for monitoring α1,3-fucosyltransferase IX activity.
Authors: Lunau, Nathalie and Seelhorst, Katrin and Kahl, Stefanie and Tscherch, Kathrin and Stacke, Christina and Rohn, Sascha and Thiem, Joachim and Hahn, Ulrich and Meier, Chris
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2013): 17379-90
Authors: Lunau, Nathalie and Seelhorst, Katrin and Kahl, Stefanie and Tscherch, Kathrin and Stacke, Christina and Rohn, Sascha and Thiem, Joachim and Hahn, Ulrich and Meier, Chris
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2013): 17379-90