logo
AAT Bioquest

ATTO 594 acid

Product key features

  • Ex/Em: 602/621 nm
  • Extinction coefficient: 120,000 cm-1M-1
  • High Quantum Yield & Stability: Provides bright fluorescence with high photostability and thermal resilience
  • Superior Water Solubility: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications
  • Ideal for Super-Resolution Imaging: Suited for PALM, dSTORM, and STED techniques in high-resolution microscopy

Product description

ATTO 594 is a bright, red fluorescent dye characterized by a strong absorption, high fluorescence quantum yield, and exceptional thermal and photostability. The dye exhibits superior water solubility and hydrophilicity, facilitating its use in various aqueous environments. ATTO 594 is optimally excited within the 560-615 nm range, making it compatible with both 561 nm and 594 nm laser lines commonly used in advanced fluorescence imaging systems. Upon conjugation to biomolecules, ATTO 594 becomes anionic, carrying a net charge of -1, which may influence its binding characteristics and performance in assays. Its photostability and brightness make it particularly suited for high-resolution techniques like single-molecule detection and super-resolution microscopy, including PALM, dSTORM, and STED. Additionally, ATTO 594 is highly compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other fluorescence-based assays, supporting its broad utility in complex biological studies.

ATTO 594 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing ATTO 594 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ATTO 488 acid499520900000.800.220.09
ATTO 532 acid5315521150000.900.220.11
ATTO 647 acid6466661200000.200.080.04
ATTO 647N acid6456631500000.6510.060.05
ATTO 514 acid510531115,0000.850.210.08
ATTO 565 acid5625891200000.900.270.12
ATTO 390 acid39047524000.900.460.09
ATTO 425 acid438484450000.900.190.17
ATTO 495 acid497525800000.20.450.37
ATTO 550 acid5535741200000.800.230.10
ATTO 590 acid5926211200000.800.390.43
ATTO 610 acid6156321500000.700.030.06
ATTO 620 acid61964112000010.510.040.06
ATTO 633 acid6296511300000.6410.040.05
ATTO 655 acid6616791250000.310.240.08
ATTO 680 acid6796961250000.300.300.17
ATTO 700 acid6997151200000.250.260.41
ATTO 594 TCO6026211200000.850.260.51
ATTO 594 Tetrazine6026211200000.850.260.51
Show More (10)

References

View all 50 references: Citation Explorer
Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells.
Authors: Xiao, Wen and Yeom, Kyu-Hyeon and Lin, Chia-Ho and Black, Douglas L
Journal: RNA (New York, N.Y.) (2023)
Measuring Photophysical Transition Rates with Fluorescence Correlation Spectroscopy and Antibunching.
Authors: Sakhapov, Damir and Gregor, Ingo and Karedla, Narain and Enderlein, Jörg
Journal: The journal of physical chemistry letters (2022): 4823-4830
Combining Fluorescence Fluctuations and Photobleaching to Quantify Surface Density.
Authors: Sefkow-Werner, Julius and Migliorini, Elisa and Picart, Catherine and Wahyuni, Dwiria and Wang, Irène and Delon, Antoine
Journal: Analytical chemistry (2022): 6521-6528
A Model of F-actin Organization in Granuloreticulopodia in Foraminifera: Morphogenetic and Evolutionary Implications from Novel Fluorescent and Polarised Light Observations.
Authors: Goleń, Jan and Tyszka, Jarosław and Godos, Karolina and Janse, Max
Journal: Protist (2022): 125886
Fractional CO2 laser ablation leads to enhanced permeation of a fluorescent dye in healthy and mycotic nails-An imaging investigation of laser-tissue effects and their impact on ungual drug delivery.
Authors: Ortner, Vinzent Kevin and Nguyen, Nhi and Brewer, Jonathan R and Solovyeva, Vita and Haedersdal, Merete and Philipsen, Peter Alshede
Journal: Lasers in surgery and medicine (2022): 861-874
Page updated on December 6, 2024

Ordering information

Price
Unit size
Catalog Number2858
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

1008.35

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.26

Correction Factor (280 nm)

0.51

Extinction coefficient (cm -1 M -1)

120000

Excitation (nm)

602

Emission (nm)

621

Quantum yield

0.85

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Product Image
Product Image
Gallery Image 1
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as ATTO 594 acid, can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.