Calculator
ATTO 594 acid
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1008.35 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.26 |
| Correction Factor (280 nm) | 0.51 |
| Extinction coefficient (cm -1 M -1) | 120000 |
| Excitation (nm) | 602 |
| Emission (nm) | 621 |
| Quantum yield | 0.85 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| ATTO 594 maleimide |
| Overview |  SDSProtocol |
Molecular weight 1008.35 | Correction Factor (260 nm) 0.26 | Correction Factor (280 nm) 0.51 | Extinction coefficient (cm -1 M -1) 120000 | Excitation (nm) 602 | Emission (nm) 621 | Quantum yield 0.85 |
ATTO 594 is a fluorescent rhodamine dye. It has strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and excellent water solubility. It can be used for labeling of DNA, RNA or proteins, and for applications such as single-molecule detection, high-resolution microscopy, flow cytometry, and fluorescence in-situ hybridization. It has an absorption peak at ~600 nm and an emission peak at ~630 nm. This ATTO 594 acid can be conjugated to biomolecules with activation by EDC or other catalysts.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ATTO 594 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 99.172 µL | 495.86 µL | 991.719 µL | 4.959 mL | 9.917 mL |
| 5 mM | 19.834 µL | 99.172 µL | 198.344 µL | 991.719 µL | 1.983 mL |
| 10 mM | 9.917 µL | 49.586 µL | 99.172 µL | 495.86 µL | 991.719 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.26 |
| Correction Factor (280 nm) | 0.51 |
| Extinction coefficient (cm -1 M -1) | 120000 |
| Excitation (nm) | 602 |
| Emission (nm) | 621 |
| Quantum yield | 0.85 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| ATTO 488 acid | 499 | 520 | 90000 | 0.80 | 0.25 | 0.10 |
| ATTO 532 acid | 531 | 552 | 115000 | 0.90 | 0.22 | 0.11 |
| ATTO 647 acid | 646 | 666 | 120000 | 0.20 | 0.08 | 0.04 |
| ATTO 647N acid | 645 | 663 | 150000 | 0.651 | 0.06 | 0.05 |
| ATTO 514 acid | 510 | 531 | 115,000 | 0.85 | 0.21 | 0.08 |
Images
Figure 1. ATTO 594 is a fluorescent rhodamine dye. It has strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and excellent water solubility. The ATTO 594 acid can be conjugated to biomolecules with activation by EDC or other catalysts.
References
View all 50 references: Citation Explorer
Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells.
Authors: Xiao, Wen and Yeom, Kyu-Hyeon and Lin, Chia-Ho and Black, Douglas L
Journal: RNA (New York, N.Y.) (2023)
Authors: Xiao, Wen and Yeom, Kyu-Hyeon and Lin, Chia-Ho and Black, Douglas L
Journal: RNA (New York, N.Y.) (2023)
Measuring Photophysical Transition Rates with Fluorescence Correlation Spectroscopy and Antibunching.
Authors: Sakhapov, Damir and Gregor, Ingo and Karedla, Narain and Enderlein, Jörg
Journal: The journal of physical chemistry letters (2022): 4823-4830
Authors: Sakhapov, Damir and Gregor, Ingo and Karedla, Narain and Enderlein, Jörg
Journal: The journal of physical chemistry letters (2022): 4823-4830
Combining Fluorescence Fluctuations and Photobleaching to Quantify Surface Density.
Authors: Sefkow-Werner, Julius and Migliorini, Elisa and Picart, Catherine and Wahyuni, Dwiria and Wang, Irène and Delon, Antoine
Journal: Analytical chemistry (2022): 6521-6528
Authors: Sefkow-Werner, Julius and Migliorini, Elisa and Picart, Catherine and Wahyuni, Dwiria and Wang, Irène and Delon, Antoine
Journal: Analytical chemistry (2022): 6521-6528
A Model of F-actin Organization in Granuloreticulopodia in Foraminifera: Morphogenetic and Evolutionary Implications from Novel Fluorescent and Polarised Light Observations.
Authors: Goleń, Jan and Tyszka, Jarosław and Godos, Karolina and Janse, Max
Journal: Protist (2022): 125886
Authors: Goleń, Jan and Tyszka, Jarosław and Godos, Karolina and Janse, Max
Journal: Protist (2022): 125886
Fractional CO2 laser ablation leads to enhanced permeation of a fluorescent dye in healthy and mycotic nails-An imaging investigation of laser-tissue effects and their impact on ungual drug delivery.
Authors: Ortner, Vinzent Kevin and Nguyen, Nhi and Brewer, Jonathan R and Solovyeva, Vita and Haedersdal, Merete and Philipsen, Peter Alshede
Journal: Lasers in surgery and medicine (2022): 861-874
Authors: Ortner, Vinzent Kevin and Nguyen, Nhi and Brewer, Jonathan R and Solovyeva, Vita and Haedersdal, Merete and Philipsen, Peter Alshede
Journal: Lasers in surgery and medicine (2022): 861-874
Fluorescent opioid receptor ligands as tools to study opioid receptor function.
Authors: Giakomidi, Despina and Bird, Mark F and Guerrini, Remo and Calo, Girolamo and Lambert, David G
Journal: Journal of pharmacological and toxicological methods (2022): 107132
Authors: Giakomidi, Despina and Bird, Mark F and Guerrini, Remo and Calo, Girolamo and Lambert, David G
Journal: Journal of pharmacological and toxicological methods (2022): 107132
Two-Photon-Excited Single-Molecule Fluorescence Enhanced by Gold Nanorod Dimers.
Authors: Lu, Xuxing and Punj, Deep and Orrit, Michel
Journal: Nano letters (2022): 4215-4222
Authors: Lu, Xuxing and Punj, Deep and Orrit, Michel
Journal: Nano letters (2022): 4215-4222
Scanning Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy: Conformational Dynamics of DNA Holliday Junction from Microsecond to Subsecond.
Authors: Heo, Wooseok and Hasegawa, Kazuto and Okamoto, Kenji and Sako, Yasushi and Ishii, Kunihiko and Tahara, Tahei
Journal: The journal of physical chemistry letters (2022): 1249-1257
Authors: Heo, Wooseok and Hasegawa, Kazuto and Okamoto, Kenji and Sako, Yasushi and Ishii, Kunihiko and Tahara, Tahei
Journal: The journal of physical chemistry letters (2022): 1249-1257
Lipophilic probe behavior in microemulsions evaluated by fluorescence correlation spectroscopy.
Authors: Yamamoto, Johtaro and Suzuki, Yoshio and Ogawa, Yoshikatsu and Kamata, Tomoyuki and Hashimoto, Hinako and Kunitake, Masashi and Kato, Dai
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2022): 401-408
Authors: Yamamoto, Johtaro and Suzuki, Yoshio and Ogawa, Yoshikatsu and Kamata, Tomoyuki and Hashimoto, Hinako and Kunitake, Masashi and Kato, Dai
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2022): 401-408
Atto488-Agitoxin 2-A Fluorescent Ligand with Increased Selectivity for Kv1.3 Channel Binding Site.
Authors: Denisova, Kristina R and Orlov, Nikita A and Yakimov, Sergey A and Kirpichnikov, Mikhail P and Feofanov, Alexey V and Nekrasova, Oksana V
Journal: Bioengineering (Basel, Switzerland) (2022)
Authors: Denisova, Kristina R and Orlov, Nikita A and Yakimov, Sergey A and Kirpichnikov, Mikhail P and Feofanov, Alexey V and Nekrasova, Oksana V
Journal: Bioengineering (Basel, Switzerland) (2022)