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ATTO 594 alkyne

Product key features

  • Ex/Em: 602/621 nm
  • Extinction coefficient: 120,000 cm-1M-1
  • Reactive group: alkyne
  • Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
  • High Quantum Yield & Stability: Provides bright fluorescence with high photostability and thermal resilience
  • Superior Water Solubility: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Product description

ATTO 594 is a bright, red fluorescent dye characterized by a strong absorption, high fluorescence quantum yield, and exceptional thermal and photostability. The dye exhibits superior water solubility and hydrophilicity, facilitating its use in various aqueous environments. ATTO 594 is optimally excited within the 560-615 nm range, making it compatible with both 561 nm and 594 nm laser lines commonly used in advanced fluorescence imaging systems. Upon conjugation to biomolecules, ATTO 594 becomes anionic, carrying a net charge of -1, which may influence its binding characteristics and performance in assays. Its photostability and brightness make it particularly suited for high-resolution techniques like single-molecule detection and super-resolution microscopy, including PALM, dSTORM, and STED. Additionally, ATTO 594 is highly compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other fluorescence-based assays, supporting its broad utility in complex biological studies.

The alkyne derivative of ATTO 594 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.

Spectrum

References

View all 7 references: Citation Explorer
The new live imagers MitoMM1/2 for mitochondrial visualization.
Authors: Maeda, Miwa and Suzuki, Mayu and Takashima, Shigeo and Sasaki, Tsutomu and Oh-Hashi, Kentaro and Takemori, Hiroshi
Journal: Biochemical and biophysical research communications (2021): 50-54
DNA-templated control of chirality and efficient energy transport in supramolecular DNA architectures with aggregation-induced emission.
Authors: Ucar, Hülya and Wagenknecht, Hans-Achim
Journal: Chemical science (2021): 10048-10053
Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter.
Authors: Mathiassen, Patricia P M and Menon, Anant K and Pomorski, Thomas Günther
Journal: Scientific reports (2021): 14364
Importance of probe design for bioanalysis of oligonucleotides using hybridization-based LC-fluorescence assays.
Authors: Ji, Yuhuan and Liu, Yijiang and Xia, Wanhong and Behling, Alexander and Meng, Min and Bennett, Patrick and Wang, Laixin
Journal: Bioanalysis (2019): 1917-1925
The effect of local dynamics of Atto 390-labeled lysozyme on fluorescence anisotropy modeling.
Authors: Babcock, Jeremiah J and Brancaleon, Lorenzo
Journal: Biopolymers (2015): 285-95
Page updated on April 15, 2025

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Catalog Number2841
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Physical properties

Molecular weight

944.22

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.26

Correction Factor (280 nm)

0.51

Extinction coefficient (cm -1 M -1)

120000

Excitation (nm)

602

Emission (nm)

621

Quantum yield

0.85

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Product Image
Product Image
Gallery Image 1
Schematic illustrating the azide–alkyne cycloaddition (“click chemistry”) between an alkyne‐functionalized dye and an azide‐presenting biomolecule. In the presence of a copper catalyst (CuAAC) or under strain‐promoted conditions (SPAAC), the azide and alkyne react to form a stable 1,2,3‐triazole linkage. This highly selective and robust reaction proceeds under mild conditions, tolerates a wide range of functional groups, and is frequently used to label proteins, nucleic acids, and other biomolecules for imaging, proteomics, and high‐throughput assays.