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ATTO 594 TCO

Product key features

  • Ex/Em: 602/621 nm
  • Extinction coefficient: 120,000 cm-1M-1
  • Reactive group: TCO
  • Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules with high yield
  • Robust Quantum Yield & Stability: Provides bright fluorescence with high photostability and thermal resilience
  • Superior Water Solubility: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Product description

ATTO 594 is a bright, red fluorescent dye characterized by a strong absorption, high fluorescence quantum yield, and exceptional thermal and photostability. The dye exhibits superior water solubility and hydrophilicity, facilitating its use in various aqueous environments. ATTO 594 is optimally excited within the 560-615 nm range, making it compatible with both 561 nm and 594 nm laser lines commonly used in advanced fluorescence imaging systems. Upon conjugation to biomolecules, ATTO 594 becomes anionic, carrying a net charge of -1, which may influence its binding characteristics and performance in assays. Its photostability and brightness make it particularly suited for high-resolution techniques like single-molecule detection and super-resolution microscopy, including PALM, dSTORM, and STED. Additionally, ATTO 594 is highly compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other fluorescence-based assays, supporting its broad utility in complex biological studies.

ATTO 594 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 594 TCO58760320000010.5310.050.04
ATTO 594 acid6026211200000.850.260.51
ATTO 594 maleimide6026211200000.850.260.51
ATTO 390 TCO39047524000.900.460.09
ATTO 425 TCO438484450000.900.190.17
ATTO 495 TCO497525800000.20.450.37
ATTO 550 TCO5535741200000.800.230.10
ATTO 590 TCO5926211200000.800.390.43
ATTO 610 TCO6156321500000.700.030.06
ATTO 620 TCO61964112000010.510.040.06
ATTO 633 TCO6296511300000.6410.040.05
ATTO 655 TCO6616791250000.310.240.08
ATTO 488 TCO499520900000.800.220.09
ATTO 647N TCO6456631500000.6510.060.05
ATTO 680 TCO6796961250000.300.300.17
ATTO 700 TCO6997151200000.250.260.41
ATTO 594 alkyne6026211200000.850.260.51
ATTO 594 Tetrazine6026211200000.850.260.51
ATTO 532 TCO5315521150000.900.220.11
ATTO 565 TCO5625891200000.900.270.12
Show More (11)

References

View all 33 references: Citation Explorer
Scanning Switch-off Microscopy for Super-Resolution Fluorescence Imaging.
Authors: Gao, Zhaoshuai and Hou, Shangguo and Deng, Suhui and Liang, Le and Wang, Fei and Guo, Linjie and Fang, Weina and Li, Qian and Kang, Bin and Chen, Hong-Yuan and Fan, Chunhai
Journal: Nano letters (2024): 12125-12132
Time-resolved fluorescence anisotropy with Atto 488-labeled phytochrome Agp1 from Agrobacterium fabrum.
Authors: Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman
Journal: Photochemistry and photobiology (2024): 561-572
Analysis of photon trajectories from diffusing single molecules.
Authors: Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung
Journal: The Journal of chemical physics (2023)
Curvature preference of cubic CsPbBr3 quantum dots embedded onto phospholipid bilayer membranes.
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
Interactions of the Kv1.1 Channel with Peptide Pore Blockers: A Fluorescent Analysis on Mammalian Cells.
Authors: Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V
Journal: Membranes (2023)
Page updated on December 6, 2024

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Catalog Number2843
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Physical properties

Molecular weight

1115.46

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.26

Correction Factor (280 nm)

0.51

Extinction coefficient (cm -1 M -1)

120000

Excitation (nm)

602

Emission (nm)

621

Quantum yield

0.85

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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