Calculator
ATTO 647 acid
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 706.81 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.08 |
| Correction Factor (280 nm) | 0.04 |
| Extinction coefficient (cm -1 M -1) | 120000 |
| Excitation (nm) | 646 |
| Emission (nm) | 666 |
| Quantum yield | 0.20 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
Alternative formats
| ATTO 647 maleimide |
| Overview |  SDSProtocol |
Molecular weight 706.81 | Correction Factor (260 nm) 0.08 | Correction Factor (280 nm) 0.04 | Extinction coefficient (cm -1 M -1) 120000 | Excitation (nm) 646 | Emission (nm) 666 | Quantum yield 0.20 |
ATTO 647 is rhodamine-based fluorescent label for the red spectral region that has similar spectral properties to Cy5. It has strong absorption, high fluorescence quantum yield, high thermal and photostability, and its absorption and fluorescence are independent of pH from 2 to 11. ATTO 647 is suitable for single-molecule detection applications, high-resolution microscopy (such as SIM, STED), flow cytometry (FACS) and fluorescence in-situ hybridization (FISH).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ATTO 647 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 141.481 µL | 707.404 µL | 1.415 mL | 7.074 mL | 14.148 mL |
| 5 mM | 28.296 µL | 141.481 µL | 282.961 µL | 1.415 mL | 2.83 mL |
| 10 mM | 14.148 µL | 70.74 µL | 141.481 µL | 707.404 µL | 1.415 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.08 |
| Correction Factor (280 nm) | 0.04 |
| Extinction coefficient (cm -1 M -1) | 120000 |
| Excitation (nm) | 646 |
| Emission (nm) | 666 |
| Quantum yield | 0.20 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| ATTO 488 acid | 499 | 520 | 90000 | 0.80 | 0.25 | 0.10 |
| ATTO 532 acid | 531 | 552 | 115000 | 0.90 | 0.22 | 0.11 |
| ATTO 647N acid | 645 | 663 | 150000 | 0.651 | 0.06 | 0.05 |
| ATTO 594 acid | 602 | 621 | 120000 | 0.85 | 0.26 | 0.51 |
| ATTO 514 acid | 510 | 531 | 115,000 | 0.85 | 0.21 | 0.08 |
Images
Figure 1. With EDAC or other equivalent activating coupling agents, fluorescent ATTO dyes can react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Citations
View all 21 citations: Citation Explorer
Single-molecule enzymatic reaction dynamics and mechanisms of GPX3 and TRXh9 from Arabidopsis thaliana
Authors: Kuang, Yanmin and Guo, Xing and Guo, Aiyu and Ran, Xia and He, Yulu and Zhang, Yu and Guo, Lijun
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2020): 118778
Authors: Kuang, Yanmin and Guo, Xing and Guo, Aiyu and Ran, Xia and He, Yulu and Zhang, Yu and Guo, Lijun
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2020): 118778
A novel nanocomposite based on fluorescent turn-on gold nanostars for near-infrared photothermal therapy and self-theranostic caspase-3 imaging of glioblastoma tumor cell
Authors: Wang, J., Zhou, Z., Zhang, F., Xu, H., Chen, W., Jiang, T.
Journal: Colloids Surf B Biointerfaces (2018): 303-311
Authors: Wang, J., Zhou, Z., Zhang, F., Xu, H., Chen, W., Jiang, T.
Journal: Colloids Surf B Biointerfaces (2018): 303-311
Cell-permeable organic fluorescent probes for live-cell long-term super-resolution imaging reveal lysosome-mitochondrion interactions
Authors: Han, Y., Li, M., Qiu, F., Zhang, M., Zhang, Y. H.
Journal: Nat Commun (2017): 1307
Authors: Han, Y., Li, M., Qiu, F., Zhang, M., Zhang, Y. H.
Journal: Nat Commun (2017): 1307
Field-Controlled Charge Separation in a Conductive Matrix at the Single-Molecule Level: Toward Controlling Single-Molecule Fluorescence Intermittency
Authors: Kennes, K., Dedecker, P., Hutchison, J. A., Fron, E., Uji, I. H., Hofkens, J., Van der Auweraer, M.
Journal: ACS Omega (2016): 1383-1392
Authors: Kennes, K., Dedecker, P., Hutchison, J. A., Fron, E., Uji, I. H., Hofkens, J., Van der Auweraer, M.
Journal: ACS Omega (2016): 1383-1392
Determination of equilibrium and rate constants for complex formation by fluorescence correlation spectroscopy supplemented by dynamic light scattering and Taylor dispersion analysis
Authors: Zhang, X., Poniewierski, A., Jelinska, A., Zagozdzon, A., Wisniewska, A., Hou, S., Holyst, R.
Journal: Soft Matter (2016): 8186-8194
Authors: Zhang, X., Poniewierski, A., Jelinska, A., Zagozdzon, A., Wisniewska, A., Hou, S., Holyst, R.
Journal: Soft Matter (2016): 8186-8194
A Cystine Knot Peptide Targeting Integrin alphavbeta6 for Photoacoustic and Fluorescence Imaging of Tumors in Living Subjects
Authors: Zhang, C., Kimura, R., Abou-Elkacem, L., Levi, J., Xu, L., Gambhir, S. S.
Journal: J Nucl Med (2016): 1629-1634
Authors: Zhang, C., Kimura, R., Abou-Elkacem, L., Levi, J., Xu, L., Gambhir, S. S.
Journal: J Nucl Med (2016): 1629-1634
Tracking structural transitions of bovine serum albumin in surfactant solutions by fluorescence correlation spectroscopy and fluorescence lifetime analysis
Authors: Zhang, X., Poniewierski, A., Hou, S., Sozanski, K., Wisniewska, A., Wieczorek, S. A., Kalwarczyk, T., Sun, L., Holyst, R.
Journal: Soft Matter (2015): 2512-8
Authors: Zhang, X., Poniewierski, A., Hou, S., Sozanski, K., Wisniewska, A., Wieczorek, S. A., Kalwarczyk, T., Sun, L., Holyst, R.
Journal: Soft Matter (2015): 2512-8
The effect of local dynamics of Atto 390-labeled lysozyme on fluorescence anisotropy modeling
Authors: Babcock, J. J., Brancaleon, L.
Journal: Biopolymers (2015): 285-95
Authors: Babcock, J. J., Brancaleon, L.
Journal: Biopolymers (2015): 285-95
Application of single molecule fluorescence microscopy to characterize the penetration of a large amphiphilic molecule in the stratum corneum of human skin
Authors: Volz, P., Boreham, A., Wolf, A., Kim, T. Y., Balke, J., Frombach, J., Hadam, S., Afraz, Z., Rancan, F., Blume-Peytavi, U., Vogt, A., Alexiev, U.
Journal: Int J Mol Sci (2015): 6960-77
Authors: Volz, P., Boreham, A., Wolf, A., Kim, T. Y., Balke, J., Frombach, J., Hadam, S., Afraz, Z., Rancan, F., Blume-Peytavi, U., Vogt, A., Alexiev, U.
Journal: Int J Mol Sci (2015): 6960-77
The key role of geminate electron-hole pair recombination in the delayed fluorescence in rhodamine 6G and ATTO-532
Authors: Aydemir, M., Jankus, V., Dias, F. B., Monkman, A.
Journal: Phys Chem Chem Phys (2014): 21543-9
Authors: Aydemir, M., Jankus, V., Dias, F. B., Monkman, A.
Journal: Phys Chem Chem Phys (2014): 21543-9
References
View all 1 references: Citation Explorer
Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates
Authors: Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugl and RP., undefined
Journal: J Histochem Cytochem (2003): 1699
Authors: Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugl and RP., undefined
Journal: J Histochem Cytochem (2003): 1699