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ATTO 647 acid

Product key features

  • Ex/Em: 646/666 nm
  • Extinction coefficient: 120,000 cm-1M-1
  • Robust Quantum Yield: Offers bright fluorescence, making it suitable for sensitive detection in various imaging techniques
  • High Stability: Ensures consistent fluorescence with excellent photostability and thermal stability
  • Excellent Hydrophilicity: Readily dissolves in aqueous environments for optimal performance in biological assays
  • Zwitterionic: Minimizes non-specific binding to biological surfaces, reducing background noise in live-cell imaging and super-resolution microscopy

Product description

ATTO 647 is a rhodamine-derived fluorescent dye optimized for applications within the red spectral region, offering spectral characteristics similar to Cy5. Its characterized by a high molar absorptivity, robust fluorescence quantum yield, high photostability, and good aqueous solubility, making it well-suited for demanding experimental conditions. The dye is highly hydrophilic, with an excitation maximum between the 615-660 nm range, making it compatible with the 633 nm He:Ne laser, 647 nm Krypton-Ion laser, and 650 nm diode laser. As a zwitterionic molecule, ATTO 647 carries a net neutral charge. The dye is stable under physiological pH conditions and in buffers with a pH of up to 8, though it gradually degrades at higher pH levels. ATTO 647 is ideal for advanced applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays.

ATTO 647 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing ATTO 647 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ATTO 488 acid499520900000.800.220.09
ATTO 532 acid5315521150000.900.220.11
ATTO 647N acid6456631500000.6510.060.05
ATTO 594 acid6026211200000.850.260.51
ATTO 514 acid510531115,0000.850.210.08
ATTO 565 acid5625891200000.900.270.12
ATTO 390 acid39047524000.900.460.09
ATTO 425 acid438484450000.900.190.17
ATTO 495 acid497525800000.20.450.37
ATTO 550 acid5535741200000.800.230.10
ATTO 590 acid5926211200000.800.390.43
ATTO 610 acid6156321500000.700.030.06
ATTO 620 acid61964112000010.510.040.06
ATTO 633 acid6296511300000.6410.040.05
ATTO 655 acid6616791250000.310.240.08
ATTO 680 acid6796961250000.300.300.17
ATTO 700 acid6997151200000.250.260.41
ATTO 647 Tetrazine6466661200000.200.080.04
iFluor® 647 acid65667025000010.2510.030.03
Show More (10)

Citations

View all 21 citations: Citation Explorer
Single-molecule enzymatic reaction dynamics and mechanisms of GPX3 and TRXh9 from Arabidopsis thaliana
Authors: Kuang, Yanmin and Guo, Xing and Guo, Aiyu and Ran, Xia and He, Yulu and Zhang, Yu and Guo, Lijun
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2020): 118778
A novel nanocomposite based on fluorescent turn-on gold nanostars for near-infrared photothermal therapy and self-theranostic caspase-3 imaging of glioblastoma tumor cell
Authors: Wang, J., Zhou, Z., Zhang, F., Xu, H., Chen, W., Jiang, T.
Journal: Colloids Surf B Biointerfaces (2018): 303-311
Cell-permeable organic fluorescent probes for live-cell long-term super-resolution imaging reveal lysosome-mitochondrion interactions
Authors: Han, Y., Li, M., Qiu, F., Zhang, M., Zhang, Y. H.
Journal: Nat Commun (2017): 1307
Field-Controlled Charge Separation in a Conductive Matrix at the Single-Molecule Level: Toward Controlling Single-Molecule Fluorescence Intermittency
Authors: Kennes, K., Dedecker, P., Hutchison, J. A., Fron, E., Uji, I. H., Hofkens, J., Van der Auweraer, M.
Journal: ACS Omega (2016): 1383-1392
Determination of equilibrium and rate constants for complex formation by fluorescence correlation spectroscopy supplemented by dynamic light scattering and Taylor dispersion analysis
Authors: Zhang, X., Poniewierski, A., Jelinska, A., Zagozdzon, A., Wisniewska, A., Hou, S., Holyst, R.
Journal: Soft Matter (2016): 8186-8194

References

View all 1 references: Citation Explorer
Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates
Authors: Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugl and RP., undefined
Journal: J Histochem Cytochem (2003): 1699
Page updated on December 6, 2024

Ordering information

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Catalog Number2850
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Additional ordering information

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Physical properties

Molecular weight

706.81

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.08

Correction Factor (280 nm)

0.04

Extinction coefficient (cm -1 M -1)

120000

Excitation (nm)

646

Emission (nm)

666

Quantum yield

0.20

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Product Image
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Gallery Image 1
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as ATTO 647 acid, can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.

Alternative formats