ATTO 514 azide

• Ex/Em: 510/531 nm
• Extinction coefficient: 115,000 cm^-1M^-1
• Reactive group: azide
• Click Chemistry Conjugation: Efficiently and rapidly labels terminal alkynes via CuAAC and copper-free SPAAC
• Superior Brightness & Stability: Provides robust quantum yield with high photostability and thermal stability
• Excellent Hydrophilicity: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

ATTO 514 azide is manufactured by AAT Bioquest for research and development use. ATTO 514 azide is used to label terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry with the bright, green fluorescent ATTO 514 dye. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.

ATTO 514 is a hydrophilic fluorescent dye characterized by excellent aqueous solubility, strong molar absorptivity, high fluorescence quantum yield, and notable thermal and photostability. These properties make it well-suited for advanced imaging techniques, including single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. ATTO 514 is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other fluorescence-based assays. The dye is optimally excited within the 510–535 nm range, with the 514 nm line of an Argon-Ion laser providing efficient excitation.