AzoDye-3 Alkyne

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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Molecular weight	697.76
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

AzoDye-3 Succinimidyl Ester

AzoDye-3 Amine

AzoDye-3 Maleimide

AzoDye-3 Azide

Overview SDS Protocol

Molecular weight

697.76

AzoDye-3 alkyne is an excellent building block for labeling azide-containing molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-3 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	143.316 µL	716.579 µL	1.433 mL	7.166 mL	14.332 mL
5 mM	28.663 µL	143.316 µL	286.632 µL	1.433 mL	2.866 mL
10 mM	14.332 µL	71.658 µL	143.316 µL	716.579 µL	1.433 mL

Molarity calculator

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Spectrum

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Spectral properties

Images

Figure 1. AzoDye-3 alkyne is an excellent building block for labeling azide-containing molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients.

References

View all 50 references: Citation Explorer

A sensitive and facile microRNA detection based on CRISPR-Cas12a coupled with strand displacement amplification.
Authors: Wang, Xin and Qin, Yuxin and Huang, Yong and Hu, Kun and Zhao, Shulin and Tian, Jianniao
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2022): 121476

Development and validation of a PCR-free nucleic acid testing method for RNA viruses based on linear molecular beacon probes.
Authors: Du, Fuyu and Zhang, Weijie and Yao, Huimin and Xia, Yuqiong and Zhang, Xianghan and Yang, Peng and Ning, Pengbo
Journal: Journal of nanobiotechnology (2022): 269

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Authors: Mahani, Mohamad and Khakbaz, Faeze and Ju, Huangxian
Journal: Analytical and bioanalytical chemistry (2022): 2505-2512

FRET-based hACE2 receptor mimic peptide conjugated nanoprobe for simple detection of SARS-CoV-2.
Authors: Kang, Byunghoon and Lee, Youngjin and Lim, Jaewoo and Yong, Dongeun and Ki Choi, Young and Woo Yoon, Sun and Seo, Seungbeom and Jang, Soojin and Uk Son, Seong and Kang, Taejoon and Jung, Juyeon and Lee, Kyu-Sun and Kim, Myung Hee and Lim, Eun-Kyung
Journal: Chemical engineering journal (Lausanne, Switzerland : 1996) (2022): 136143

Rapid and Highly Sensitive Detection of Target DNA Related to COVID-19 Virus With a Fluorescent Bio-conjugated Probe via a FRET Mechanism.
Authors: Bardajee, Ghasem Rezanejade and Zamani, Mohammadreza and Sharifi, Mahdieh and Rezanejad, Habib and Motallebi, Mostafa
Journal: Journal of fluorescence (2022)

Highly sensitive and efficient fluorescent sensing for Hg2+ detection based on triple-helix molecular switch and exonuclease III-assisted amplification.
Authors: Zhong, Miao and Yang, Shengyuan and Chen, Lei and Liu, Chen and Shi, Jiao and Liang, Hao and Xiao, Xilin and Li, Le and Liu, Jinquan
Journal: Analytica chimica acta (2022): 339751

Combining functional hairpin probes with disordered cleavage of CRISPR/Cas12a protease to screen for B lymphocytic leukemia.
Authors: Li, Hongbo and Zhao, Weihua and Pu, Jiamei and Zhong, Shengliang and Wang, Suqin and Yu, Ruqin
Journal: Biosensors & bioelectronics (2022): 113941

A persistent luminescence resonance energy transfer-based molecular beacon probe for the highly sensitive detection of microRNA in biological samples.
Authors: Wang, Xiuhua and Wang, Yuhui and Chen, Shu and Fu, Pan and Lin, Yuanbin and Ye, Shuyuan and Long, Yunfei and Gao, Guosheng and Zheng, Jianping
Journal: Biosensors & bioelectronics (2022): 113849

Fluorescence biosensing of the leukemia gene by combining Target-Programmed controllable signal inspiring engineering.
Authors: Li, Hongbo and Pu, Jiamei and Wang, Suqin and Yu, Ruqin
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2022): 121579

Multiplex qPCR for differentiation of Mycobacterium avium subspecies paratuberculosis in active and passive infection of goats.
Authors: Pachoori, Anjali and Gururaj, K and Sachan, Supriya and Sharma, Deepansh
Journal: Applied microbiology and biotechnology (2022): 4705-4717