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AzoDye-3 Alkyne

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Physical properties
Molecular weight697.76
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
AzoDye-3 alkyne is an excellent building block for labeling azide-containing molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-3 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM143.316 µL716.579 µL1.433 mL7.166 mL14.332 mL
5 mM28.663 µL143.316 µL286.632 µL1.433 mL2.866 mL
10 mM14.332 µL71.658 µL143.316 µL716.579 µL1.433 mL

Molarity calculator

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Spectral properties



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