AzoDye-3 Amine
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 816.81 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
AzoDye-3 Succinimidyl Ester |
AzoDye-3 Maleimide |
AzoDye-3 Azide |
AzoDye-3 Alkyne |
Overview | SDSProtocol |
See also: Förster Resonance Energy Transfer (FRET)
Molecular weight 816.81 |
AzoDye-3 amine is an excellent building block for labeling carbonyl-containing molecules. AzoDye-3 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-3 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-3's ability to form stable, non-fluorescent complexes with a fluorophore.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-3 Amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 122.427 µL | 612.137 µL | 1.224 mL | 6.121 mL | 12.243 mL |
5 mM | 24.485 µL | 122.427 µL | 244.855 µL | 1.224 mL | 2.449 mL |
10 mM | 12.243 µL | 61.214 µL | 122.427 µL | 612.137 µL | 1.224 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
References
View all 50 references: Citation Explorer
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