Bio-16-UTP [Biotin-16-UTP] *1 mM*

Bio-16-UTP is biotinylated UTP that can be used as a substrate in in vitro transcription reactions with a variety of RNA polymerases, including T7 RNA polymerase, SP6 RNA polymerase, or T3 RNA polymerase. Biotinylated RNA probes may be used in place of radioactively labeled RNA probes in many applications, including in situ hybridization and chromosome mapping. Bio-16-UTP can be detected using a variety of methods based on streptavidin binding. Biotin-bound streptavidin can be detected directly, using fluorescently labeled streptavidin, or indirectly, using a streptavidin-conjugated enzyme assay or appropriately labeled or conjugated anti-streptavidin antibody.