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Bio-16-UTP [Biotin-16-UTP] *1 mM*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 963.78 |
| Solvent | Water |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
| Overview |  SDSProtocol |
See also: Biotin and Streptavidin
Molecular weight 963.78 |
Bio-16-UTP is biotinylated UTP that can be used as a substrate in in vitro transcription reactions with a variety of RNA polymerases, including T7 RNA polymerase, SP6 RNA polymerase, or T3 RNA polymerase. Biotinylated RNA probes may be used in place of radioactively labeled RNA probes in many applications, including in situ hybridization and chromosome mapping. Bio-16-UTP can be detected using a variety of methods based on streptavidin binding. Biotin-bound streptavidin can be detected directly, using fluorescently labeled streptavidin, or indirectly, using a streptavidin-conjugated enzyme assay or appropriately labeled or conjugated anti-streptavidin antibody.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Bio-16-UTP [Biotin-16-UTP] *1 mM* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 103.758 µL | 518.791 µL | 1.038 mL | 5.188 mL | 10.376 mL |
| 5 mM | 20.752 µL | 103.758 µL | 207.516 µL | 1.038 mL | 2.075 mL |
| 10 mM | 10.376 µL | 51.879 µL | 103.758 µL | 518.791 µL | 1.038 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. Biotin-16-UTP is a modified nucleotide used for labeling RNA molecules. It is a derivative of uridine triphosphate (UTP) that contains a biotin molecule attached to the 16-carbon position of the pyrimidine ring. Biotin-16-UTP is often used in in vitro transcription reactions to incorporate biotin-labeled nucleotides into RNA molecules. This allows for the purification and detection of RNA molecules using streptavidin-coated beads or plates, which bind specifically to biotin. Biotin-16-UTP can also be used for in situ hybridization experiments to visualize the location of RNA molecules in cells or tissues.
References
View all 8 references: Citation Explorer
Electrochemical biosensor for microRNA detection based on poly(U) polymerase mediated isothermal signal amplification.
Authors: Zhou, Yunlei and Yin, Huanshun and Li, Jie and Li, Bingchen and Li, Xue and Ai, Shiyun and Zhang, Xiansheng
Journal: Biosensors & bioelectronics (2016): 79-85
Authors: Zhou, Yunlei and Yin, Huanshun and Li, Jie and Li, Bingchen and Li, Xue and Ai, Shiyun and Zhang, Xiansheng
Journal: Biosensors & bioelectronics (2016): 79-85
Isolation of translating ribosomes containing peptidyl-tRNAs for functional and structural analyses.
Authors: Shirole, Nitin and Balasubramanian, Sreeram and Yanofsky, Charles and Cruz-Vera, Luis
Journal: Journal of visualized experiments : JoVE (2011)
Authors: Shirole, Nitin and Balasubramanian, Sreeram and Yanofsky, Charles and Cruz-Vera, Luis
Journal: Journal of visualized experiments : JoVE (2011)
Duplex in situ hybridization in the study of gene co-regulation in the vertebrate brain.
Authors: Pinaud, Raphael and Jeong, Jin K
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 115-29
Authors: Pinaud, Raphael and Jeong, Jin K
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 115-29
rRNA probe-based cell fishing of bacteria.
Authors: Stoffels, M and Ludwig, W and Schleifer, K H
Journal: Environmental microbiology (1999): 259-71
Authors: Stoffels, M and Ludwig, W and Schleifer, K H
Journal: Environmental microbiology (1999): 259-71
An in situ hybridization histochemistry technique allowing simultaneous visualization by the use of confocal microscopy of three cellular mRNA species in individual neurons.
Authors: Grino, M and Zamora, A J
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1998): 753-9
Authors: Grino, M and Zamora, A J
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1998): 753-9
Scaffold attachment regions in centromere-associated DNA.
Authors: Strissel, P L and Espinosa, R and Rowley, J D and Swift, H
Journal: Chromosoma (1996): 122-33
Authors: Strissel, P L and Espinosa, R and Rowley, J D and Swift, H
Journal: Chromosoma (1996): 122-33
Identification of neutral endopeptidase mRNA in human nasal mucosa.
Authors: Baraniuk, J N and Ohkubo, K and Kwon, O J and Mak, J and Rohde, J and Kaliner, M A and Durham, S R and Barnes, P J
Journal: Journal of applied physiology (Bethesda, Md. : 1985) (1993): 272-9
Authors: Baraniuk, J N and Ohkubo, K and Kwon, O J and Mak, J and Rohde, J and Kaliner, M A and Durham, S R and Barnes, P J
Journal: Journal of applied physiology (Bethesda, Md. : 1985) (1993): 272-9
Preferential distribution of active RNA polymerase II molecules in the nuclear periphery.
Authors: Clark, R F and Cho, K W and Weinmann, R and Hamkalo, B A
Journal: Gene expression (1991): 61-70
Authors: Clark, R F and Cho, K W and Weinmann, R and Hamkalo, B A
Journal: Gene expression (1991): 61-70