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Bio-16-UTP [Biotin-16-UTP] *1 mM*

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Physical properties
Molecular weight963.78
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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OverviewpdfSDSpdfProtocol


Molecular weight
963.78
Bio-16-UTP is biotinylated UTP that can be used as a substrate in in vitro transcription reactions with a variety of RNA polymerases, including T7 RNA polymerase, SP6 RNA polymerase, or T3 RNA polymerase. Biotinylated RNA probes may be used in place of radioactively labeled RNA probes in many applications, including in situ hybridization and chromosome mapping. Bio-16-UTP can be detected using a variety of methods based on streptavidin binding. Biotin-bound streptavidin can be detected directly, using fluorescently labeled streptavidin, or indirectly, using a streptavidin-conjugated enzyme assay or appropriately labeled or conjugated anti-streptavidin antibody.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Bio-16-UTP [Biotin-16-UTP] *1 mM* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM103.758 µL518.791 µL1.038 mL5.188 mL10.376 mL
5 mM20.752 µL103.758 µL207.516 µL1.038 mL2.075 mL
10 mM10.376 µL51.879 µL103.758 µL518.791 µL1.038 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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References


View all 8 references: Citation Explorer
Electrochemical biosensor for microRNA detection based on poly(U) polymerase mediated isothermal signal amplification.
Authors: Zhou, Yunlei and Yin, Huanshun and Li, Jie and Li, Bingchen and Li, Xue and Ai, Shiyun and Zhang, Xiansheng
Journal: Biosensors & bioelectronics (2016): 79-85
Isolation of translating ribosomes containing peptidyl-tRNAs for functional and structural analyses.
Authors: Shirole, Nitin and Balasubramanian, Sreeram and Yanofsky, Charles and Cruz-Vera, Luis
Journal: Journal of visualized experiments : JoVE (2011)
Duplex in situ hybridization in the study of gene co-regulation in the vertebrate brain.
Authors: Pinaud, Raphael and Jeong, Jin K
Journal: Methods in molecular biology (Clifton, N.J.) (2010): 115-29
rRNA probe-based cell fishing of bacteria.
Authors: Stoffels, M and Ludwig, W and Schleifer, K H
Journal: Environmental microbiology (1999): 259-71
An in situ hybridization histochemistry technique allowing simultaneous visualization by the use of confocal microscopy of three cellular mRNA species in individual neurons.
Authors: Grino, M and Zamora, A J
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1998): 753-9
Scaffold attachment regions in centromere-associated DNA.
Authors: Strissel, P L and Espinosa, R and Rowley, J D and Swift, H
Journal: Chromosoma (1996): 122-33
Identification of neutral endopeptidase mRNA in human nasal mucosa.
Authors: Baraniuk, J N and Ohkubo, K and Kwon, O J and Mak, J and Rohde, J and Kaliner, M A and Durham, S R and Barnes, P J
Journal: Journal of applied physiology (Bethesda, Md. : 1985) (1993): 272-9
Preferential distribution of active RNA polymerase II molecules in the nuclear periphery.
Authors: Clark, R F and Cho, K W and Weinmann, R and Hamkalo, B A
Journal: Gene expression (1991): 61-70