logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Bio-16-UTP
Biotin-16-UTP; 1 mM

Bio-16-UTP is biotinylated UTP that can be used as a substrate in in vitro transcription reactions with a variety of RNA polymerases, including T7 RNA polymerase, SP6 RNA polymerase, or T3 RNA polymerase. Biotinylated RNA probes may be used in place of radioactively labeled RNA probes in many applications, including in situ hybridization and chromosome mapping. Bio-16-UTP can be detected using a variety of methods based on streptavidin binding. Biotin-bound streptavidin can be detected directly, using fluorescently labeled streptavidin, or indirectly, using a streptavidin-conjugated enzyme assay or appropriately labeled or conjugated anti-streptavidin antibody.

Biotin-16-UTP is a modified nucleotide used for labeling RNA molecules. It is a derivative of uridine triphosphate (UTP) that contains a biotin molecule attached to the 16-carbon position of the pyrimidine ring. Biotin-16-UTP is often used in in vitro transcription reactions to incorporate biotin-labeled nucleotides into RNA molecules. This allows for the purification and detection of RNA molecules using streptavidin-coated beads or plates, which bind specifically to biotin. Biotin-16-UTP can also be used for in situ hybridization experiments to visualize the location of RNA molecules in cells or tissues.
Biotin-16-UTP is a modified nucleotide used for labeling RNA molecules. It is a derivative of uridine triphosphate (UTP) that contains a biotin molecule attached to the 16-carbon position of the pyrimidine ring. Biotin-16-UTP is often used in in vitro transcription reactions to incorporate biotin-labeled nucleotides into RNA molecules. This allows for the purification and detection of RNA molecules using streptavidin-coated beads or plates, which bind specifically to biotin. Biotin-16-UTP can also be used for in situ hybridization experiments to visualize the location of RNA molecules in cells or tissues.
protocol
Protocol
COA
CatalogSize
Price
Quantity
1711025 nmoles
Price
17111100 nmoles
Price
 
Physical properties
Molecular weight963.78
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 5, 2025