AAT Bioquest

Biotin-11-dATP *1 mM in Tris Buffer (pH 7.5)*

Product Image
Product Image
Gallery Image 1
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight882.71
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Related products
Biotin *CAS 58-85-5*
Biotin, succinimidyl ester *CAS 35013-72-0*
Biotin ethylenediamine *CAS 1217450-40-2*
Biotin cadaverine
Biotin C2 maleimide
Biotin-4-fluorescein *CAS 1032732-74-3*
Biotin hydrazide *CAS 66640-86-6*
Biotin-X NTA [Biotin-X nitrilotriacetic acid, potassium salt] *CAS 856661-92-2*
Biotin-X, succinimidyl ester *CAS 72040-63-2*
Biotin PEG2 amine *CAS 138529-46-1*
Biotin PEG2 maleimide *CAS 305372-39-8*
Biotin PEG2 succinimidyl ester
Biotin-PEG3-azide *CAS 875770-34-6*
Biotin Azide
Biotin Alkyne *CAS 773888-45-2*
Biotin PEG4 succinimidyl ester
ReadiView™ biotin acid
ReadiView™ biotin amine
ReadiView™ biotin hydrazide
ReadiView™ biotin maleimide
ReadiView™ biotin succinimidyl ester
ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology*
Amplite® Colorimetric Biotin Quantitation Kit
Biotin-16-dUTP *1 mM in TE Buffer (pH 7.5)* *CAS 136632-31-0*
Biotin-20-dUTP *1 mM in TE Buffer (pH 7.5)*
Cal-520®-Biotin Conjugate
Phalloidin-Biotin Conjugate
Biotin-14-dCTP *1 mM in Tris Buffer (pH 7.5)*
Biotin PEG3 amine
Annexin V-Biotin conjugate
N6-Methyladenosine-Biotin conjugate
Biotin Styramide *Superior Replacement for Biotin Tyramide*
Cy5 biotin conjugate
Biotin-cAMP conjugate
2',3'-cGAMP-Biotin conjugate
Cy5.5 biotin conjugate
HRP-Biotin Conjugate
Biotin NTA
Biotin-X IDA
ReadiCleave™ SSL biotin NHS ester
Biotin-dT Phosphoramidite
ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit
ReadiLink™ Biotin Oligo and ssDNA Labeling Kit
AF532 PEG4 biotin conjugate
Biotin PEG 4 Alkyne
Cy3 biotin conjugate
Cy7 biotin conjugate
Biotin C2 Azide
mFluor™ UV 375 Biotin Conjugate
mFluor™ UV 460 Biotin Conjugate
mFluor™ Violet 500 Biotin Conjugate
mFluor™ Violet 540 Biotin Conjugate
mFluor™ Red 780 Biotin Conjugate
Biotin Phosphoramidite
Bio-16-UTP [Biotin-16-UTP] *1 mM*
Biotin PEG4 amine
mFluor™ Violet 450-PEG4-Biotin Conjugate
Acridinium Biotin Conjugate
FastClick™ Biotin Azide
FastClick™ Biotin Alkyne
ReadiLeave™ Reversible Biotin Succinimidyl Ester
ReadiLeave™ Reversible Biotin Maleimide
ReadiLeave™ Reversible Biotin Azide
ReadiLeave™ Reversible Biotin Alkyne
ReadiLeave™ Reversible Biotin Amine
Xylazine-Biotin Conjugate
Amplite® Rapid Colorimetric Biotin Quantitation Kit *Optimized to Use with Nanodrop*
Amplite® Fluorimetric Biotin Quantitation Kit
Portelite™ Rapid Fluorimetric Biotin Quantitation Kit *Optimized to Use with CytoCite™ and Qubit™ Fluorometers*
Biotin-PEG2-azide *CAS 945633-30-7*
Show More (62)


Molecular weight
Botin-modified deoxyadenosine 5'-triphosphates are widely used for various non-radioactive DNA labeling reactions, including nick translation, random prime labeling, cDNA labeling, and 3’-end labeling. Biotinylated probes have been shown to hybridize with homologous nucleic acid at the same rate and extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by avidin and streptavidin. Biotin-11-dATP *1 mM in Tris Buffer (pH 7.5)* can be enzymatically incorporated into DNA via nick-translation, random priming, 3'-end terminal labeling, or during PCR. The number '11' refers to the number of carbon atoms in the backbone of the linker between dATP and biotin. The more effective interaction between biotin and avidin will occur with longer linkers, whereas shorter linkers will lead to more efficient incorporation of dATP into DNA. It is suggested that the linker '11' length is optimal for most applications. Biotin-11-dATP *1 mM in Tris Buffer (pH 7.5)* produces biotinylated DNA probes in various hybridization applications, including Southern blots, Northern blots, dot blots, fixed cells, and tissues. It is chemically equivalent to NEL540001EA of PerkinElmer (PE).


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Biotin-11-dATP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM113.287 µL566.437 µL1.133 mL5.664 mL11.329 mL
5 mM22.657 µL113.287 µL226.575 µL1.133 mL2.266 mL
10 mM11.329 µL56.644 µL113.287 µL566.437 µL1.133 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



View all 17 references: Citation Explorer
Measurement of differential chromatin interactions with absolute quantification of architecture (AQuA-HiChIP).
Authors: Gryder, Berkley E and Khan, Javed and Stanton, Benjamin Z
Journal: Nature protocols (2020): 1209-1236
Highly sensitive and selective electrochemical detection of Hg(2+) through surface-initiated enzymatic polymerization.
Authors: Mei, Chenyang and Lin, Dajie and Fan, Chengchao and Liu, Aili and Wang, Shun and Wang, Jichang
Journal: Biosensors & bioelectronics (2016): 105-110
Ultrasensitive electrochemical DNA sensor based on the target induced structural switching and surface-initiated enzymatic polymerization.
Authors: Wan, Ying and Wang, Pengjuan and Su, Yan and Zhu, Xinhua and Yang, Shulin and Lu, Jianxin and Gao, Jimin and Fan, Chunhai and Huang, Qing
Journal: Biosensors & bioelectronics (2014): 231-6
A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors.
Authors: Wan, Ying and Xu, Hui and Su, Yan and Zhu, Xinhua and Song, Shiping and Fan, Chunhai
Journal: Biosensors & bioelectronics (2013): 526-31
Oxidative DNA injury after experimental intracerebral hemorrhage.
Authors: Nakamura, Takehiro and Keep, Richard F and Hua, Ya and Hoff, Julian T and Xi, Guohua
Journal: Brain research (2005): 30-6
Impact of hypoglycemia and diabetes on CNS: correlation of mitochondrial oxidative stress with DNA damage.
Authors: Singh, Puneet and Jain, Anu and Kaur, Gurcharan
Journal: Molecular and cellular biochemistry (2004): 153-9
Oxidative brain injury from extravasated erythrocytes after intracerebral hemorrhage.
Authors: Wu, Jimin and Hua, Ya and Keep, Richard F and Schallert, Timothy and Hoff, Julian T and Xi, Guohua
Journal: Brain research (2002): 45-52
Detection of single- and double-strand DNA breaks after traumatic brain injury in rats: comparison of in situ labeling techniques using DNA polymerase I, the Klenow fragment of DNA polymerase I, and terminal deoxynucleotidyl transferase.
Authors: Clark RSB, and Chen, M and Kochanek, P M and Watkins, S C and Jin, K L and Draviam, R and Nathaniel, P D and Pinto, R and Marion, D W and Graham, S H
Journal: Journal of neurotrauma (2001): 675-89
Spatio-temporal profile of DNA fragmentation and its relationship to patterns of epileptiform activity following focally evoked limbic seizures.
Authors: Henshall, D C and Sinclair, J and Simon, R P
Journal: Brain research (2000): 290-302
Induction of oxidative DNA damage in the peri-infarct region after permanent focal cerebral ischemia.
Authors: Nagayama, T and Lan, J and Henshall, D C and Chen, D and O'Horo, C and Simon, R P and Chen, J
Journal: Journal of neurochemistry (2000): 1716-28