Biotin-11-dGTP *1 mM in Tris Buffer (pH 7.5)*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 898.71 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
Molecular weight 898.71 |
The biotin-modified deoxyguanosine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by avidin and streptavidin. Biotin-11-dGTP can be enzymatically incorporated into DNA via nick-translation, random priming, 3'-end terminal labeling or in the process of PCR. The number '11' is the number of carbon atoms in the backbone of the linker between dGTP and biotin. The longer the linker is, the more effective interaction of biotin with avidin occurs. On the other hand, the shorter the linker is, the more effective incorporation of dGTP into DNA. It is suggested the length of linker '11' is optimal for most applications. Biotin-11-dGTP is used to produce biotinylated DNA probes in a variety of hybridization applications including Southern blots, Northern blots, dot blots, fixed cells, and tissues. It is chemically equivalent to NEL541001EA of PerkinElmer (PE).
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Biotin-11-dGTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 111.271 µL | 556.353 µL | 1.113 mL | 5.564 mL | 11.127 mL |
5 mM | 22.254 µL | 111.271 µL | 222.541 µL | 1.113 mL | 2.225 mL |
10 mM | 11.127 µL | 55.635 µL | 111.271 µL | 556.353 µL | 1.113 mL |
Molarity calculator
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References
View all 7 references: Citation Explorer
Base-Excision-Repair-Induced Construction of a Single Quantum-Dot-Based Sensor for Sensitive Detection of DNA Glycosylase Activity.
Authors: Wang, Li-Juan and Ma, Fei and Tang, Bo and Zhang, Chun-Yang
Journal: Analytical chemistry (2016): 7523-9
Authors: Wang, Li-Juan and Ma, Fei and Tang, Bo and Zhang, Chun-Yang
Journal: Analytical chemistry (2016): 7523-9
PRINS for mapping single-copy genes.
Authors: Tharapel, Avirachan T and Wachtel, Stephen S
Journal: Methods in molecular biology (Clifton, N.J.) (2006): 59-67
Authors: Tharapel, Avirachan T and Wachtel, Stephen S
Journal: Methods in molecular biology (Clifton, N.J.) (2006): 59-67
Chromosomal localization of single copy genes SRY and SOX3 by primed in situ labeling (PRINS).
Authors: Kadandale, J S and Tunca, Y and Tharapel, A T
Journal: Microbial & comparative genomics (2000): 71-4
Authors: Kadandale, J S and Tunca, Y and Tharapel, A T
Journal: Microbial & comparative genomics (2000): 71-4
Quantification of telomerase activity by direct scintillation counting.
Authors: Kazmer, S and Pan, K M and Vassilev, L
Journal: Journal of biochemical and biophysical methods (1999): 113-7
Authors: Kazmer, S and Pan, K M and Vassilev, L
Journal: Journal of biochemical and biophysical methods (1999): 113-7
Telomerase assay using biotinylated-primer extension and magnetic separation of the products.
Authors: Sun, D and Hurley, L H and Von Hoff, D D
Journal: BioTechniques (1998): 1046-51
Authors: Sun, D and Hurley, L H and Von Hoff, D D
Journal: BioTechniques (1998): 1046-51
Universal cloning and direct sequencing of rearranged antibody V genes using C region primers, biotin-captured cDNA and one-side PCR.
Authors: Heinrichs, A and Milstein, C and Gherardi, E
Journal: Journal of immunological methods (1995): 241-51
Authors: Heinrichs, A and Milstein, C and Gherardi, E
Journal: Journal of immunological methods (1995): 241-51
Purification of DNA fragments containing excision-repair patches from human cells using streptavidin-biotin.
Authors: Larone, G E and Hunting, D J
Journal: Mutation research (1991): 273-80
Authors: Larone, G E and Hunting, D J
Journal: Mutation research (1991): 273-80