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Biotin-11-dGTP *1 mM in Tris Buffer (pH 7.5)*

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Physical properties
Molecular weight898.71
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Molecular weight
898.71
The biotin-modified deoxyguanosine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by avidin and streptavidin. Biotin-11-dGTP can be enzymatically incorporated into DNA via nick-translation, random priming, 3'-end terminal labeling or in the process of PCR. The number '11' is the number of carbon atoms in the backbone of the linker between dGTP and biotin. The longer the linker is, the more effective interaction of biotin with avidin occurs. On the other hand, the shorter the linker is, the more effective incorporation of dGTP into DNA. It is suggested the length of linker '11' is optimal for most applications. Biotin-11-dGTP is used to produce biotinylated DNA probes in a variety of hybridization applications including Southern blots, Northern blots, dot blots, fixed cells, and tissues. It is chemically equivalent to NEL541001EA of PerkinElmer (PE).

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Biotin-11-dGTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM111.271 µL556.353 µL1.113 mL5.564 mL11.127 mL
5 mM22.254 µL111.271 µL222.541 µL1.113 mL2.225 mL
10 mM11.127 µL55.635 µL111.271 µL556.353 µL1.113 mL

Molarity calculator

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References


View all 7 references: Citation Explorer
Base-Excision-Repair-Induced Construction of a Single Quantum-Dot-Based Sensor for Sensitive Detection of DNA Glycosylase Activity.
Authors: Wang, Li-Juan and Ma, Fei and Tang, Bo and Zhang, Chun-Yang
Journal: Analytical chemistry (2016): 7523-9
PRINS for mapping single-copy genes.
Authors: Tharapel, Avirachan T and Wachtel, Stephen S
Journal: Methods in molecular biology (Clifton, N.J.) (2006): 59-67
Chromosomal localization of single copy genes SRY and SOX3 by primed in situ labeling (PRINS).
Authors: Kadandale, J S and Tunca, Y and Tharapel, A T
Journal: Microbial & comparative genomics (2000): 71-4
Quantification of telomerase activity by direct scintillation counting.
Authors: Kazmer, S and Pan, K M and Vassilev, L
Journal: Journal of biochemical and biophysical methods (1999): 113-7
Telomerase assay using biotinylated-primer extension and magnetic separation of the products.
Authors: Sun, D and Hurley, L H and Von Hoff, D D
Journal: BioTechniques (1998): 1046-51
Universal cloning and direct sequencing of rearranged antibody V genes using C region primers, biotin-captured cDNA and one-side PCR.
Authors: Heinrichs, A and Milstein, C and Gherardi, E
Journal: Journal of immunological methods (1995): 241-51
Purification of DNA fragments containing excision-repair patches from human cells using streptavidin-biotin.
Authors: Larone, G E and Hunting, D J
Journal: Mutation research (1991): 273-80