Biotin-11-dGTP *1 mM in Tris Buffer (pH 7.5)*

Biotin-11-dGTP

1 mM in Tris Buffer (pH 7.5)

The biotin-modified deoxyguanosine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by avidin and streptavidin. Biotin-11-dGTP can be enzymatically incorporated into DNA via nick-translation, random priming, 3'-end terminal labeling or in the process of PCR. The number '11' is the number of carbon atoms in the backbone of the linker between dGTP and biotin. The longer the linker is, the more effective interaction of biotin with avidin occurs. On the other hand, the shorter the linker is, the more effective incorporation of dGTP into DNA. It is suggested the length of linker '11' is optimal for most applications. Biotin-11-dGTP is used to produce biotinylated DNA probes in a variety of hybridization applications including Southern blots, Northern blots, dot blots, fixed cells, and tissues. It is chemically equivalent to NEL541001EA of PerkinElmer (PE).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17015	25 nmoles	Price

References

View all 7 references: Citation Explorer

Base-Excision-Repair-Induced Construction of a Single Quantum-Dot-Based Sensor for Sensitive Detection of DNA Glycosylase Activity.

Authors:

Wang, Li-Juan and Ma, Fei and Tang, Bo and Zhang, Chun-Yang

Journal:

Analytical chemistry (2016): 7523-9

PRINS for mapping single-copy genes.

Authors:

Tharapel, Avirachan T and Wachtel, Stephen S

Journal:

Methods in molecular biology (Clifton, N.J.) (2006): 59-67

Chromosomal localization of single copy genes SRY and SOX3 by primed in situ labeling (PRINS).

Authors:

Kadandale, J S and Tunca, Y and Tharapel, A T

Journal:

Microbial & comparative genomics (2000): 71-4

Quantification of telomerase activity by direct scintillation counting.

Authors:

Kazmer, S and Pan, K M and Vassilev, L

Journal:

Journal of biochemical and biophysical methods (1999): 113-7

Telomerase assay using biotinylated-primer extension and magnetic separation of the products.

Authors:

Sun, D and Hurley, L H and Von Hoff, D D

Journal:

BioTechniques (1998): 1046-51

Physical properties

Molecular weight	898.71
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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