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Biotin-PEG2-azide *CAS 945633-30-7*

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Physical properties
Molecular weight400.50
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Desiccated
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Molecular weight
Biotin-PEG2-Azide *CAS 945633-30-7* is a non-cleavable, azide-activated biotinylation reagent designed for use in bioconjugation applications. It selectively reacts with terminal alkynes to form stable triazole linkages, either via copper-catalyzed click reactions or Cu-free click reactions with strained cyclooctynes (e.g., DBCO or BCN compounds). Additionally, it can react with phosphine-labeled molecules through Staudinger chemistry. These reactions are bioorthogonal, meaning they do not interfere with biological systems. The inclusion of a hydrophilic PEG2 spacer in Biotin-PEG2-Azide enhances its aqueous solubility and reduces steric hindrance, particularly when binding to avidin molecules. This reagent is particularly useful for detecting cellular alkyne cholesterol analogs and for preparing multifunctional benzophenone linkers used in pull-down assays, photoaffinity labeling, and double-click stapling techniques.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin-PEG2-azide *CAS 945633-30-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM249.688 µL1.248 mL2.497 mL12.484 mL24.969 mL
5 mM49.938 µL249.688 µL499.376 µL2.497 mL4.994 mL
10 mM24.969 µL124.844 µL249.688 µL1.248 mL2.497 mL

Molarity calculator

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View all 29 references: Citation Explorer
An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation.
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Maleimide-Based Chemical Proteomics for Quantitative Analysis of Cysteine Reactivity.
Authors: McConnell, Evan W and Smythers, Amanda L and Hicks, Leslie M
Journal: Journal of the American Society for Mass Spectrometry (2020)
Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale.
Authors: Flaxman, Hope A and Miyamoto, David K and Woo, Christina M
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Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP).
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