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Biotin-PEG2-azide *CAS 945633-30-7*

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Physical properties
Molecular weight400.50
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Desiccated
UNSPSC12352200
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OverviewpdfSDSpdfProtocol


CAS
945633-30-7
Molecular weight
400.50
Biotin-PEG2-Azide *CAS 945633-30-7* is a non-cleavable, azide-activated biotinylation reagent designed for use in bioconjugation applications. It selectively reacts with terminal alkynes to form stable triazole linkages, either via copper-catalyzed click reactions or Cu-free click reactions with strained cyclooctynes (e.g., DBCO or BCN compounds). Additionally, it can react with phosphine-labeled molecules through Staudinger chemistry. These reactions are bioorthogonal, meaning they do not interfere with biological systems. The inclusion of a hydrophilic PEG2 spacer in Biotin-PEG2-Azide enhances its aqueous solubility and reduces steric hindrance, particularly when binding to avidin molecules. This reagent is particularly useful for detecting cellular alkyne cholesterol analogs and for preparing multifunctional benzophenone linkers used in pull-down assays, photoaffinity labeling, and double-click stapling techniques.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotin-PEG2-azide *CAS 945633-30-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM249.688 µL1.248 mL2.497 mL12.484 mL24.969 mL
5 mM49.938 µL249.688 µL499.376 µL2.497 mL4.994 mL
10 mM24.969 µL124.844 µL249.688 µL1.248 mL2.497 mL

Molarity calculator

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References


View all 29 references: Citation Explorer
An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation.
Authors: Rajkumar, Shreenidhi and Dixon, Danielle and Lipchik, Andrew M and Gruber, Joshua J
Journal: Journal of visualized experiments : JoVE (2024)
Rapid cell type-specific nascent proteome labeling in Drosophila.
Authors: Villalobos-Cantor, Stefanny and Barrett, Ruth M and Condon, Alec F and Arreola-Bustos, Alicia and Rodriguez, Kelsie M and Cohen, Michael S and Martin, Ian
Journal: eLife (2023)
Capture, Release, and Identification of Newly Synthesized Proteins for Improved Profiling of Functional Translatomes.
Authors: Phillips, Nancy J and Vinaithirthan, Bala M and Oses-Prieto, Juan A and Chalkley, Robert J and Burlingame, Alma L
Journal: Molecular & cellular proteomics : MCP (2023): 100497
Selective Removal of Unhydrolyzed Monolinked Peptides from Enriched Crosslinked Peptides To Improve the Coverage of Protein Complex Analysis.
Authors: An, Yuxin and Zhao, Qun and Gao, Hang and Zhao, Lili and Li, Xiao and Zhang, Xiaodan and Liang, Zhen and Zhang, Lihua and Zhang, Yukui
Journal: Analytical chemistry (2022): 3904-3913
Ultrastructural localization of de novo synthesized phosphatidylcholine in yeast cells by freeze-fracture electron microscopy.
Authors: Tsuji, Takuma and Fujimoto, Toyoshi
Journal: STAR protocols (2021): 100990
Profiling and Validation of Live-Cell Protein Methylation with Engineered Enzymes and Methionine Analogues.
Authors: Weiss, Nicole and Seneviranthe, Chamara and Jiang, Ming and Wang, Ke and Luo, Minkui
Journal: Current protocols (2021): e213
Maleimide-Based Chemical Proteomics for Quantitative Analysis of Cysteine Reactivity.
Authors: McConnell, Evan W and Smythers, Amanda L and Hicks, Leslie M
Journal: Journal of the American Society for Mass Spectrometry (2020)
Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale.
Authors: Flaxman, Hope A and Miyamoto, David K and Woo, Christina M
Journal: Current protocols in chemical biology (2019): e75
Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP).
Authors: Dadfar, Seyed Mohammad Mahdi and Sekula-Neuner, Sylwia and Trouillet, Vanessa and Liu, Hui-Yu and Kumar, Ravi and Powell, Annie K and Hirtz, Michael
Journal: Beilstein journal of nanotechnology (2019): 2505-2515
Lipopolysaccharide Upregulates Palmitoylated Enzymes of the Phosphatidylinositol Cycle: An Insight from Proteomic Studies.
Authors: Sobocińska, Justyna and Roszczenko-Jasińska, Paula and Zaręba-Kozioł, Monika and Hromada-Judycka, Aneta and Matveichuk, Orest V and Traczyk, Gabriela and Łukasiuk, Katarzyna and Kwiatkowska, Katarzyna
Journal: Molecular & cellular proteomics : MCP (2018): 233-254