Biotin-PEG2-azide *CAS 945633-30-7*

Biotin-PEG2-azide

CAS 945633-30-7

Biotin-PEG2-Azide CAS 945633-30-7 is a non-cleavable, azide-activated biotinylation reagent designed for use in bioconjugation applications. It selectively reacts with terminal alkynes to form stable triazole linkages, either via copper-catalyzed click reactions or Cu-free click reactions with strained cyclooctynes (e.g., DBCO or BCN compounds). Additionally, it can react with phosphine-labeled molecules through Staudinger chemistry. These reactions are bioorthogonal, meaning they do not interfere with biological systems. The inclusion of a hydrophilic PEG2 spacer in Biotin-PEG2-Azide enhances its aqueous solubility and reduces steric hindrance, particularly when binding to avidin molecules. This reagent is particularly useful for detecting cellular alkyne cholesterol analogs and for preparing multifunctional benzophenone linkers used in pull-down assays, photoaffinity labeling, and double-click stapling techniques.

Biotin-PEG2-Azide is an excellent alkyne-reactive building block for developing biotin probes in bioconjugation applications via the well-known click chemistry.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3025	5 mg	Price

References

View all 29 references: Citation Explorer

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation.

Authors:

Rajkumar, Shreenidhi and Dixon, Danielle and Lipchik, Andrew M and Gruber, Joshua J

Journal:

Journal of visualized experiments : JoVE (2024)

Rapid cell type-specific nascent proteome labeling in Drosophila.

Authors:

Villalobos-Cantor, Stefanny and Barrett, Ruth M and Condon, Alec F and Arreola-Bustos, Alicia and Rodriguez, Kelsie M and Cohen, Michael S and Martin, Ian

Journal:

eLife (2023)

Capture, Release, and Identification of Newly Synthesized Proteins for Improved Profiling of Functional Translatomes.

Authors:

Phillips, Nancy J and Vinaithirthan, Bala M and Oses-Prieto, Juan A and Chalkley, Robert J and Burlingame, Alma L

Journal:

Molecular & cellular proteomics : MCP (2023): 100497

Selective Removal of Unhydrolyzed Monolinked Peptides from Enriched Crosslinked Peptides To Improve the Coverage of Protein Complex Analysis.

Authors:

An, Yuxin and Zhao, Qun and Gao, Hang and Zhao, Lili and Li, Xiao and Zhang, Xiaodan and Liang, Zhen and Zhang, Lihua and Zhang, Yukui

Journal:

Analytical chemistry (2022): 3904-3913

Ultrastructural localization of de novo synthesized phosphatidylcholine in yeast cells by freeze-fracture electron microscopy.

Authors:

Tsuji, Takuma and Fujimoto, Toyoshi

Journal:

STAR protocols (2021): 100990

Physical properties

Molecular weight	400.50
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Desiccated
UNSPSC	12352200
CAS	945633-30-7

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 25, 2025