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Biotin-X IDA

Chemical structure for Biotin-X IDA.
Chemical structure for Biotin-X IDA.
Ordering information
Price ()
Catalog Number12630
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for recombinant proteins fused to a polyhistidine-tag. Transition metal ions immobilized to a matrix through a chelating ligand interact with the polyhistidine-tag, effectively sequestering the fused protein from a sample. Nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA) are two such ligands commonly used in commercially available resins. AAT Bioquest offer a variety of NTA building blocks for developing either NTA-based purification and detection. IDA is complimentary to NTA. The tridentate IDA ligand requires a lower imidazole concentration to elute protein than the tetradentate NTA. IDA is a smaller molecule which can be coupled to the matrix at a higher density resulting in a higher metal loading capacity. Biotin IDA is a bifunctional reagent that can be used to detect histidine-tagged proteins immobilized. Biotin IDA can be removed from the histidine-tagged protein at pH 4.8, allowing the blot to be reanalyzed with another probe.

References


View all 9 references: Citation Explorer
Synchronized purification and immobilization of his-tagged β-glucosidase via Fe3O4/PMG core/shell magnetic nanoparticles.
Authors: Zhou, Yang and Yuan, Shaofei and Liu, Qian and Yan, Dandan and Wang, Yun and Gao, Li and Han, Juan and Shi, Haifeng
Journal: Scientific reports (2017): 41741
Grafting iminodiacetic acid on silica nanoparticles for facilitated refolding of like-charged protein and its metal-chelate affinity purification.
Authors: Liu, Hu and Dong, Xiaoyan and Sun, Yan
Journal: Journal of chromatography. A (2016): 277-83
Uniform magnetic core/shell microspheres functionalized with Ni2+-iminodiacetic acid for one step purification and immobilization of his-tagged enzymes.
Authors: Zhang, Yuting and Yang, Yongkun and Ma, Wanfu and Guo, Jia and Lin, Yao and Wang, Changchun
Journal: ACS applied materials & interfaces (2013): 2626-33
Dual affinity method for plasmid DNA purification in aqueous two-phase systems.
Authors: Barbosa, H S C and Hine, A V and Brocchini, S and Slater, N K H and Marcos, J C
Journal: Journal of chromatography. A (2010): 1429-36
Evaluation of IDA-PEVA hollow fiber membrane metal ion affinity chromatography for purification of a histidine-tagged human proinsulin.
Authors: de Aquino, Luciana Cristina Lins and de Sousa, Heloisa Ribeiro Tunes and Miranda, Everson Alves and Vilela, Luciano and Bueno, Sônia Maria Alves
Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2006): 68-76
Overproduction of Thermus sp. Strain T2 beta-galactosidase in Escherichia coli and preparation by using tailor-made metal chelate supports.
Authors: Pessela, Benevides C C and Vian, Alejandro and Mateo, César and Fernández-Lafuente, Roberto and García, José L and Guisán, José M and Carrascosa, Alfonso V
Journal: Applied and environmental microbiology (2003): 1967-72
Evidence for phosphorylation of serine 753 in CFTR using a novel metal-ion affinity resin and matrix-assisted laser desorption mass spectrometry.
Authors: Neville, D C and Rozanas, C R and Price, E M and Gruis, D B and Verkman, A S and Townsend, R R
Journal: Protein science : a publication of the Protein Society (1997): 2436-45
Cell chromatography: separation of different microbial cells using IMAC supermacroporous monolithic columns.
Authors: Dainiak, Maria B and Plieva, Fatima M and Galaev, Igor Yu and Hatti-Kaul, Rajni and Mattiasson, Bo
Journal: Biotechnology progress : 644-9
One-step purification, covalent immobilization, and additional stabilization of a thermophilic poly-His-tagged beta-galactosidase from Thermus sp. strain T2 by using novel heterofunctional chelate-epoxy Sepabeads.
Authors: Pessela, Benevides C C and Mateo, Cesar and Carrascosa, Alfonso V and Vian, Alejandro and García, José L and Rivas, German and Alfonso, Carlos and Guisan, José M and Fernández-Lafuente, Roberto
Journal: Biomacromolecules : 107-13