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Biotinyl tyramide

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Physical properties
Molecular weight363.48
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity and utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. This process is called as the catalyzed reporter deposition (CARD)The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets and the use of smaller amounts of antibodies and hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals, and can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. Biotinyl tyramide is considered to achieve maximal IHC detection among all the TSA reagents. Biotinyl tyramide has been used in immunohistochemistry, ELISA, Western blot, and in situ hybridization applications.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Biotinyl tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM275.118 µL1.376 mL2.751 mL13.756 mL27.512 mL
5 mM55.024 µL275.118 µL550.237 µL2.751 mL5.502 mL
10 mM27.512 µL137.559 µL275.118 µL1.376 mL2.751 mL

Molarity calculator

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Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy3 tyramide55556915000010.1510.070.073
Cy5 tyramide65167025000010.271, 0.420.020.03
Cy7 tyramide7567792500000.30.050.036
XFD514 tyramide51854380000-0.310.18
XFD532 tyramide534553810000.6110.240.09
Fluorescein Tyramide4985178000010.79001, 0.9520.320.35



View all 18 references: Citation Explorer
High specificity and tight spatial restriction of self-biotinylation by DNA and RNA G-Quadruplexes complexed in vitro and in vivo with Heme.
Authors: Lat, Prince Kumar and Liu, Kun and Kumar, Dev N and Wong, Kenneth K L and Verheyen, Esther M and Sen, Dipankar
Journal: Nucleic acids research (2020)
Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity.
Authors: Li, Wei and Zeng, Weiwu and Chen, Yi and Wang, Fang and Wu, Fan and Weng, Xiaocheng and Zhou, Xiang
Journal: The Analyst (2019): 4472-4476
APEX Peroxidase-Catalyzed Proximity Labeling and Multiplexed Quantitative Proteomics.
Authors: Kalocsay, Marian
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 41-55
Proximity labeling of cis-ligands of CD22/Siglec-2 reveals stepwise α2,6 sialic acid-dependent and -independent interactions.
Authors: Alborzian Deh Sheikh, Amin and Akatsu, Chizuru and Imamura, Akihiro and Abdu-Allah, Hajjaj H M and Takematsu, Hiromu and Ando, Hiromune and Ishida, Hideharu and Tsubata, Takeshi
Journal: Biochemical and biophysical research communications (2018): 854-859
Tyramide Signal Amplification Permits Immunohistochemical Analyses of Androgen Receptors in the Rat Prefrontal Cortex.
Authors: Low, Katelyn L and Ma, Chunqi and Soma, Kiran K
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2017): 295-308
Identification of Siglec Ligands Using a Proximity Labeling Method.
Authors: Chang, Lanyi and Chen, Yi-Ju and Fan, Chan-Yo and Tang, Chin-Ju and Chen, Yi-Hsiu and Low, Penk-Yeir and Ventura, Albert and Lin, Chun-Cheng and Chen, Yu-Ju and Angata, Takashi
Journal: Journal of proteome research (2017): 3929-3941
Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis.
Authors: Royston, Daniel J and Clasper, Steven and Jackson, David G
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 35-48
Development of a highly sensitive immunohistochemical method to detect neurochemical molecules in formalin-fixed and paraffin-embedded tissues from autopsied human brains.
Authors: Goto, Satoshi and Morigaki, Ryoma and Okita, Shinya and Nagahiro, Shinji and Kaji, Ryuji
Journal: Frontiers in neuroanatomy (2015): 22
Development of a sensitive ELISA for quantification of three- and four-repeat tau isoforms in tauopathies.
Authors: Luk, Connie and Giovannoni, Gavin and Williams, David R and Lees, Andrew J and de Silva, Rohan
Journal: Journal of neuroscience methods (2009): 34-42
Levels of pregnancy-associated plasma protein A in patients with coronary artery disease.
Authors: Liu, Zhi-Yuan and Zhang, Jin-Ying and Sun, Tong-Wen and Zhang, Yan-Jun and Zhang, Li and Wang, Le-Xin
Journal: Clinical and investigative medicine. Medecine clinique et experimentale (2008): E85-9