Biotinyl tyramide

For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity and utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. This process is called as the catalyzed reporter deposition (CARD)The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets and the use of smaller amounts of antibodies and hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals, and can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. Biotinyl tyramide is considered to achieve maximal IHC detection among all the TSA reagents. Biotinyl tyramide has been used in immunohistochemistry, ELISA, Western blot, and in situ hybridization applications.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
45350	5 mg	Price

References

View all 18 references: Citation Explorer

High specificity and tight spatial restriction of self-biotinylation by DNA and RNA G-Quadruplexes complexed in vitro and in vivo with Heme.

Authors:

Lat, Prince Kumar and Liu, Kun and Kumar, Dev N and Wong, Kenneth K L and Verheyen, Esther M and Sen, Dipankar

Journal:

Nucleic acids research (2020)

Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity.

Authors:

Li, Wei and Zeng, Weiwu and Chen, Yi and Wang, Fang and Wu, Fan and Weng, Xiaocheng and Zhou, Xiang

Journal:

The Analyst (2019): 4472-4476

APEX Peroxidase-Catalyzed Proximity Labeling and Multiplexed Quantitative Proteomics.

Authors:

Kalocsay, Marian

Journal:

Methods in molecular biology (Clifton, N.J.) (2019): 41-55

Proximity labeling of cis-ligands of CD22/Siglec-2 reveals stepwise α2,6 sialic acid-dependent and -independent interactions.

Authors:

Alborzian Deh Sheikh, Amin and Akatsu, Chizuru and Imamura, Akihiro and Abdu-Allah, Hajjaj H M and Takematsu, Hiromu and Ando, Hiromune and Ishida, Hideharu and Tsubata, Takeshi

Journal:

Biochemical and biophysical research communications (2018): 854-859

Tyramide Signal Amplification Permits Immunohistochemical Analyses of Androgen Receptors in the Rat Prefrontal Cortex.

Authors:

Low, Katelyn L and Ma, Chunqi and Soma, Kiran K

Journal:

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2017): 295-308

Physical properties

Molecular weight	363.48
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	41994-02-9

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 20, 2025