Fluorescein Tyramide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 495.49 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Antibodies and Proteomics, Antibody and Protein Labeling, Bioconjugation, Horseradish Peroxidase (HRP) and Poly-HRP, Immunohistochemistry (IHC), Power Styramide™ Signal Amplification (PSA™)
CAS 210236-90-1 | Molecular weight 495.49 | Absorbance (nm) 487 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 800001 | Excitation (nm) 498 | Emission (nm) 517 | Quantum yield 0.79001, 0.952 |
Fluorescein Tyramide is a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal. Under the same conditions, iFluor® 488 Styramide is a superior replacement with higher sensitivity and faster reaction speed. In addition, iFluor 488 is much more photostable, and tolerate much broad pH ranges.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein Tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 201.82 µL | 1.009 mL | 2.018 mL | 10.091 mL | 20.182 mL |
5 mM | 40.364 µL | 201.82 µL | 403.641 µL | 2.018 mL | 4.036 mL |
10 mM | 20.182 µL | 100.91 µL | 201.82 µL | 1.009 mL | 2.018 mL |
Molarity calculator
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Spectrum
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Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Fluorescein biotin | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Fluorescein dicaprylate [Fluorescein dioctanoate] | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Fluorescein DHPE | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Cy3 tyramide | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
Cy5 tyramide | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
Fluorescein aldehyde [5-FAM aldehyde] | 493 | 517 | 83000 | - | 0.32 | 0.178 |
Cy7 tyramide | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
XFD514 tyramide | 518 | 543 | 80000 | - | 0.31 | 0.18 |
XFD532 tyramide | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
Citations
View all 1 citations: Citation Explorer
Pro-inflammatory microenvironment and systemic accumulation of CXCR3+ cell exacerbate lung pathology of old rhesus macaques infected with SARS-CoV-2
Authors: Zheng, Hong-Yi and He, Xiao-Yan and Li, Wei and Song, Tian-Zhang and Han, Jian-Bao and Yang, Xiang and Liu, Feng-Liang and Luo, Rong-Hua and Tian, Ren-Rong and Feng, Xiao-Li and others,
Journal: Signal transduction and targeted therapy (2021): 1--12
Authors: Zheng, Hong-Yi and He, Xiao-Yan and Li, Wei and Song, Tian-Zhang and Han, Jian-Bao and Yang, Xiang and Liu, Feng-Liang and Luo, Rong-Hua and Tian, Ren-Rong and Feng, Xiao-Li and others,
Journal: Signal transduction and targeted therapy (2021): 1--12
References
View all 10 references: Citation Explorer
Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability.
Authors: Ilić Đurđić, Karla and Ece, Selin and Ostafe, Raluca and Vogel, Simon and Balaž, Ana Marija and Schillberg, Stefan and Fischer, Rainer and Prodanović, Radivoje
Journal: Journal of bioscience and bioengineering (2020): 664-671
Authors: Ilić Đurđić, Karla and Ece, Selin and Ostafe, Raluca and Vogel, Simon and Balaž, Ana Marija and Schillberg, Stefan and Fischer, Rainer and Prodanović, Radivoje
Journal: Journal of bioscience and bioengineering (2020): 664-671
The EMARS Reaction for Proximity Labeling.
Authors: Honke, Koichi and Miyagawa-Yamaguchi, Arisa and Kotani, Norihiro
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 1-12
Authors: Honke, Koichi and Miyagawa-Yamaguchi, Arisa and Kotani, Norihiro
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 1-12
Each GPI-anchored protein species forms a specific lipid raft depending on its GPI attachment signal.
Authors: Miyagawa-Yamaguchi, Arisa and Kotani, Norihiro and Honke, Koichi
Journal: Glycoconjugate journal (2015): 531-40
Authors: Miyagawa-Yamaguchi, Arisa and Kotani, Norihiro and Honke, Koichi
Journal: Glycoconjugate journal (2015): 531-40
Ultra-high-throughput screening method for the directed evolution of glucose oxidase.
Authors: Ostafe, Raluca and Prodanovic, Radivoje and Nazor, Jovana and Fischer, Rainer
Journal: Chemistry & biology (2014): 414-21
Authors: Ostafe, Raluca and Prodanovic, Radivoje and Nazor, Jovana and Fischer, Rainer
Journal: Chemistry & biology (2014): 414-21
Ultrahigh-throughput screening system for directed glucose oxidase evolution in yeast cells.
Authors: Prodanovic, Radivoje and Ostafe, Raluca and Scacioc, Andreea and Schwaneberg, Ulrich
Journal: Combinatorial chemistry & high throughput screening (2011): 55-60
Authors: Prodanovic, Radivoje and Ostafe, Raluca and Scacioc, Andreea and Schwaneberg, Ulrich
Journal: Combinatorial chemistry & high throughput screening (2011): 55-60
Cytochrome P450 1B1 expression in rat esophageal tumorigenesis promoted by gastric and duodenal reflux.
Authors: Devlin, Andrea H and McIlroy, Marie and McKeen, Hayley D and Bonde, Pramode and Menezes, A A Carlos and Swarbrick, Christine J and Robson, Tracy and Hirst, David G and Campbell, F Charles and McGuigan, James A and McKeown, Stephanie R
Journal: Molecular carcinogenesis (2009): 110-7
Authors: Devlin, Andrea H and McIlroy, Marie and McKeen, Hayley D and Bonde, Pramode and Menezes, A A Carlos and Swarbrick, Christine J and Robson, Tracy and Hirst, David G and Campbell, F Charles and McGuigan, James A and McKeown, Stephanie R
Journal: Molecular carcinogenesis (2009): 110-7
Laser scanning detection of FISH-labelled Escherichia coli from water samples.
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Journal: Water science and technology : a journal of the International Association on Water Pollution Research (2003): 123-9
Authors: Lepeuple, S and Delabre, K and Gilouppe, S and Intertaglia, L and de Roubin, M R
Journal: Water science and technology : a journal of the International Association on Water Pollution Research (2003): 123-9
Improved mRNA in situ hybridization on formaldehyde-fixed and paraffin-embedded tissue using signal amplification with different haptenized tyramides.
Authors: Speel, E J and Saremaslani, P and Roth, J and Hopman, A H and Komminoth, P
Journal: Histochemistry and cell biology (1998): 571-7
Authors: Speel, E J and Saremaslani, P and Roth, J and Hopman, A H and Komminoth, P
Journal: Histochemistry and cell biology (1998): 571-7
In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes.
Authors: Wagner, M and Schmid, M and Juretschko, S and Trebesius, K H and Bubert, A and Goebel, W and Schleifer, K H
Journal: FEMS microbiology letters (1998): 159-68
Authors: Wagner, M and Schmid, M and Juretschko, S and Trebesius, K H and Bubert, A and Goebel, W and Schleifer, K H
Journal: FEMS microbiology letters (1998): 159-68
Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification.
Authors: Schönhuber, W and Fuchs, B and Juretschko, S and Amann, R
Journal: Applied and environmental microbiology (1997): 3268-73
Authors: Schönhuber, W and Fuchs, B and Juretschko, S and Amann, R
Journal: Applied and environmental microbiology (1997): 3268-73