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Fluorescein Tyramide
Fluorescein Tyramide is a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal. Under the same conditions, iFluor® 488 Styramide is a superior replacement with higher sensitivity and faster reaction speed. In addition, iFluor 488 is much more photostable, and tolerate much broad pH ranges.
Chemical structure for Fluorescein Tyramide.
Chemical structure for Fluorescein Tyramide.
CatalogSize
Price
Quantity
110621 mg
Price
 
Physical properties

Molecular weight495.49
SolventDMSO
Spectral properties

Absorbance (nm)487
Correction factor (260 nm)0.32
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)498
Emission (nm)517
Quantum yield
0.7900
1
0.95
2
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
CAS210236-90-1
Instrument settings

Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on September 29, 2025