Fluorescein Tyramide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	495.49
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

210236-90-1

Molecular weight

495.49

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

80000¹

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.7900¹, 0.95²

Fluorescein Tyramide is a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal. Under the same conditions, iFluor® 488 Styramide is a superior replacement with higher sensitivity and faster reaction speed. In addition, iFluor 488 is much more photostable, and tolerate much broad pH ranges.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein Tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	201.82 µL	1.009 mL	2.018 mL	10.091 mL	20.182 mL
5 mM	40.364 µL	201.82 µL	403.641 µL	2.018 mL	4.036 mL
10 mM	20.182 µL	100.91 µL	201.82 µL	1.009 mL	2.018 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein biotin	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein dicaprylate [Fluorescein dioctanoate]	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein DHPE	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Cy3 tyramide	555	569	150000¹	0.15¹	0.07	0.073
Cy5 tyramide	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Fluorescein aldehyde [5-FAM aldehyde]	493	517	83000	-	0.32	0.178
Cy7 tyramide	756	779	250000	0.3	0.05	0.036
XFD514 tyramide	518	543	80000	-	0.31	0.18
XFD532 tyramide	534	553	81000	0.61¹	0.24	0.09

Images

Figure 1. Chemical structure for Fluorescein Tyramide.

Citations

View all 1 citations: Citation Explorer

Pro-inflammatory microenvironment and systemic accumulation of CXCR3+ cell exacerbate lung pathology of old rhesus macaques infected with SARS-CoV-2
Authors: Zheng, Hong-Yi and He, Xiao-Yan and Li, Wei and Song, Tian-Zhang and Han, Jian-Bao and Yang, Xiang and Liu, Feng-Liang and Luo, Rong-Hua and Tian, Ren-Rong and Feng, Xiao-Li and others,
Journal: Signal transduction and targeted therapy (2021): 1--12

References

View all 10 references: Citation Explorer

Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability.
Authors: Ilić Đurđić, Karla and Ece, Selin and Ostafe, Raluca and Vogel, Simon and Balaž, Ana Marija and Schillberg, Stefan and Fischer, Rainer and Prodanović, Radivoje
Journal: Journal of bioscience and bioengineering (2020): 664-671

The EMARS Reaction for Proximity Labeling.
Authors: Honke, Koichi and Miyagawa-Yamaguchi, Arisa and Kotani, Norihiro
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 1-12

Each GPI-anchored protein species forms a specific lipid raft depending on its GPI attachment signal.
Authors: Miyagawa-Yamaguchi, Arisa and Kotani, Norihiro and Honke, Koichi
Journal: Glycoconjugate journal (2015): 531-40

Ultra-high-throughput screening method for the directed evolution of glucose oxidase.
Authors: Ostafe, Raluca and Prodanovic, Radivoje and Nazor, Jovana and Fischer, Rainer
Journal: Chemistry & biology (2014): 414-21

Ultrahigh-throughput screening system for directed glucose oxidase evolution in yeast cells.
Authors: Prodanovic, Radivoje and Ostafe, Raluca and Scacioc, Andreea and Schwaneberg, Ulrich
Journal: Combinatorial chemistry & high throughput screening (2011): 55-60

Cytochrome P450 1B1 expression in rat esophageal tumorigenesis promoted by gastric and duodenal reflux.
Authors: Devlin, Andrea H and McIlroy, Marie and McKeen, Hayley D and Bonde, Pramode and Menezes, A A Carlos and Swarbrick, Christine J and Robson, Tracy and Hirst, David G and Campbell, F Charles and McGuigan, James A and McKeown, Stephanie R
Journal: Molecular carcinogenesis (2009): 110-7

Laser scanning detection of FISH-labelled Escherichia coli from water samples.
Authors: Lepeuple, S and Delabre, K and Gilouppe, S and Intertaglia, L and de Roubin, M R
Journal: Water science and technology : a journal of the International Association on Water Pollution Research (2003): 123-9

Improved mRNA in situ hybridization on formaldehyde-fixed and paraffin-embedded tissue using signal amplification with different haptenized tyramides.
Authors: Speel, E J and Saremaslani, P and Roth, J and Hopman, A H and Komminoth, P
Journal: Histochemistry and cell biology (1998): 571-7

In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes.
Authors: Wagner, M and Schmid, M and Juretschko, S and Trebesius, K H and Bubert, A and Goebel, W and Schleifer, K H
Journal: FEMS microbiology letters (1998): 159-68

Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification.
Authors: Schönhuber, W and Fuchs, B and Juretschko, S and Amann, R
Journal: Applied and environmental microbiology (1997): 3268-73