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Fluorescein Tyramide

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InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight495.49
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
0.79001, 0.952
Fluorescein Tyramide is a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal. Under the same conditions, iFluor® 488 Styramide is a superior replacement with higher sensitivity and faster reaction speed. In addition, iFluor 488 is much more photostable, and tolerate much broad pH ranges.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein Tyramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM201.82 µL1.009 mL2.018 mL10.091 mL20.182 mL
5 mM40.364 µL201.82 µL403.641 µL2.018 mL4.036 mL
10 mM20.182 µL100.91 µL201.82 µL1.009 mL2.018 mL

Molarity calculator

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Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Fluorescein biotin4985178000010.79001, 0.9520.320.35
Fluorescein dicaprylate [Fluorescein dioctanoate]4985178000010.79001, 0.9520.320.35
Fluorescein DHPE4985178000010.79001, 0.9520.320.35
Cy3 tyramide55556915000010.1510.070.073
Cy5 tyramide65167025000010.271, 0.420.020.03
Fluorescein aldehyde [5-FAM aldehyde]49351783000-0.320.178
Cy7 tyramide7567792500000.30.050.036
XFD514 tyramide51854380000-0.310.18
XFD532 tyramide534553810000.6110.240.09



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Pro-inflammatory microenvironment and systemic accumulation of CXCR3+ cell exacerbate lung pathology of old rhesus macaques infected with SARS-CoV-2
Authors: Zheng, Hong-Yi and He, Xiao-Yan and Li, Wei and Song, Tian-Zhang and Han, Jian-Bao and Yang, Xiang and Liu, Feng-Liang and Luo, Rong-Hua and Tian, Ren-Rong and Feng, Xiao-Li and others,
Journal: Signal transduction and targeted therapy (2021): 1--12


View all 10 references: Citation Explorer
Flow cytometry-based system for screening of lignin peroxidase mutants with higher oxidative stability.
Authors: Ilić Đurđić, Karla and Ece, Selin and Ostafe, Raluca and Vogel, Simon and Balaž, Ana Marija and Schillberg, Stefan and Fischer, Rainer and Prodanović, Radivoje
Journal: Journal of bioscience and bioengineering (2020): 664-671
The EMARS Reaction for Proximity Labeling.
Authors: Honke, Koichi and Miyagawa-Yamaguchi, Arisa and Kotani, Norihiro
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 1-12
Each GPI-anchored protein species forms a specific lipid raft depending on its GPI attachment signal.
Authors: Miyagawa-Yamaguchi, Arisa and Kotani, Norihiro and Honke, Koichi
Journal: Glycoconjugate journal (2015): 531-40
Ultra-high-throughput screening method for the directed evolution of glucose oxidase.
Authors: Ostafe, Raluca and Prodanovic, Radivoje and Nazor, Jovana and Fischer, Rainer
Journal: Chemistry & biology (2014): 414-21
Ultrahigh-throughput screening system for directed glucose oxidase evolution in yeast cells.
Authors: Prodanovic, Radivoje and Ostafe, Raluca and Scacioc, Andreea and Schwaneberg, Ulrich
Journal: Combinatorial chemistry & high throughput screening (2011): 55-60
Cytochrome P450 1B1 expression in rat esophageal tumorigenesis promoted by gastric and duodenal reflux.
Authors: Devlin, Andrea H and McIlroy, Marie and McKeen, Hayley D and Bonde, Pramode and Menezes, A A Carlos and Swarbrick, Christine J and Robson, Tracy and Hirst, David G and Campbell, F Charles and McGuigan, James A and McKeown, Stephanie R
Journal: Molecular carcinogenesis (2009): 110-7
Laser scanning detection of FISH-labelled Escherichia coli from water samples.
Authors: Lepeuple, S and Delabre, K and Gilouppe, S and Intertaglia, L and de Roubin, M R
Journal: Water science and technology : a journal of the International Association on Water Pollution Research (2003): 123-9
Improved mRNA in situ hybridization on formaldehyde-fixed and paraffin-embedded tissue using signal amplification with different haptenized tyramides.
Authors: Speel, E J and Saremaslani, P and Roth, J and Hopman, A H and Komminoth, P
Journal: Histochemistry and cell biology (1998): 571-7
In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes.
Authors: Wagner, M and Schmid, M and Juretschko, S and Trebesius, K H and Bubert, A and Goebel, W and Schleifer, K H
Journal: FEMS microbiology letters (1998): 159-68
Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification.
Authors: Schönhuber, W and Fuchs, B and Juretschko, S and Amann, R
Journal: Applied and environmental microbiology (1997): 3268-73