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Biotinylated goat anti-rabbit IgG (H+L)
Many biotin molecules can be conjugated to a secondary antibody with the additional advantage of streptavidin having a high affinity for biotin. Through this amplification step and having the streptavidin bound to labels such as HRP or fluorescent probes, proteins which are expressed at low levels are more likely to be detected. For most assays, the biotin-labeled secondary antibody is added first, sequentially followed by the streptavidin conjugated to a label e.g. HRP.
Image of HeLa cells. HeLa cells were incubated with (Tubulin+) or without (Control) rabbit anti-tubulin, followed by biotinylated goat anti-rabbit IgG (H&L) (Cat# 16794) stain, and finally visualized with iFluor® 555-streptavidin conjugate (Cat# 16959).
Image of HeLa cells. HeLa cells were incubated with (Tubulin+) or without (Control) rabbit anti-tubulin, followed by biotinylated goat anti-rabbit IgG (H&L) (Cat# 16794) stain, and finally visualized with iFluor® 555-streptavidin conjugate (Cat# 16959).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
167941 mg
Price
 
Physical properties
Molecular weight~150000
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 17, 2025