Name: Cell Meter™ 2-NBDG Glucose Uptake Assay Kit
Brand: AAT Bioquest
SKU: 23500
Price: 594 USD
Availability: InStock

Cell Meter™ 2-NBDG Glucose Uptake Assay Kit

Glucose metabolism, a process which converts glucose into energy, is a primary source of energy supply in most organisms. 2-NBDG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose], a fluorescently tagged glucose tracer, has been proven to effectively monitor glucose transportation in cells, as 2-NBDG transports into cells by the same glucose transporters (GLUTs) as glucose. Once 2-NBDG is uptaken in cells, it undergoes phosphorylation at C-6 position to give 2-NBDG-6-phosphate, which is well retained within the cells. Compared to other glucose tracers, such as 2-DG or FDG, 2-NBDG allows in situ measurements of 2-NBDG with high temporal and spatial resolution at single cell level. AAT Bioquest's Cell Meter™ 2-NBDG Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay for measuring glucose uptake in cultured cells. In this kit, Assay Buffer I is used to enhance the uptake and retention of 2-NBDG in cells, while Assay Buffer II can improve the signal-to-background ratio of 2-NBDG in the cells. The fluorescence signal can be monitored by fluorescence microscope or flow cytometer with a 488 nm laser and 530/30 nm emission filter (FITC channel). Cell Meter™ 2-NBDG Glucose Uptake Assay Kit is the most robust tool for monitoring glucose transporters.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
23500	200 Tests	Price

Citations

View all 7 citations: Citation Explorer

Cinobufagin Inhibits Invasion and Migration of Non-Small Cell Lung Cancer via Regulating Glucose Metabolism Reprogramming in Tumor-Associated Macrophages

Authors:

Sun, Ying and Yang, Huitong and Mei, Xue and Xia, Jinchan and Feng, Long and Gao, Jianfeng and Jiang, Wei and Jiang, Min and Hao, Xu and Feng, Yilin and others,

Journal:

Drug Design, Development and Therapy (2025): 6647--6664

Enhancing $\beta$-Cell Function and Identity in type 2 diabetes: The Protective Role of Coptis deltoidea CY Cheng et Hsiao via Glucose Metabolism Modulation and AMPK Signaling Activation

Authors:

Zhang, Shan and Zhang, Yueying and Wen, Zhige and Chen, Yupeng and Bu, Tianjie and Yan, Yanan and Ni, Qing

Journal:

Phytomedicine (2024): 155396

SDHB reduction promotes oral lichen planus by impairing mitochondrial respiratory function

Authors:

Zhang, Hui and Xu, Beiyun and Liu, Jin and Guo, Bin and Sun, Hongying and Yang, Qiaozhen

Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet $\beta$ cells in mice

Authors:

Liu, Hongyi and Ju, Anji and Dong, Xuan and Luo, Zongrui and Tang, Jiaze and Ma, Boyuan and Fu, Yan and Luo, Yongzhang

IKCa channels control breast cancer metabolism including AMPK-driven autophagy

Authors:

Gross, Dominic and Bischof, Helmut and Maier, Selina and Sporbeck, Katharina and Birkenfeld, Andreas L and Malli, Roland and Ruth, Peter and Proikas-Cezanne, Tassula and Lukowski, Robert

Journal:

Cell death \& disease (2022): 1--14

References

View all 14 references: Citation Explorer

Transport of a Fluorescent Analogue of Glucose (2-NBDG) versus Radiolabeled Sugars by Rumen Bacteria and Escherichia coli

Authors:

Tao J, Diaz RK, Teixeira CR, Hackmann TJ.

Journal:

Biochemistry (2016): 2578

2-NBDG as a marker for detecting glucose uptake in reactive astrocytes exposed to oxygen-glucose deprivation in vitro

Authors:

Chen Y, Zhang J, Zhang XY.

Journal:

J Mol Neurosci (2015): 126

Syzygium aqueum leaf extract and its bioactive compounds enhances pre-adipocyte differentiation and 2-NBDG uptake in 3T3-L1 cells

Authors:

Manaharan T, Ming CH, Palanisamy UD.

Journal:

Food Chem (2013): 354

2-NBDG fluorescence imaging of hypermetabolic circulating tumor cells in mouse xenograft model of breast cancer

Authors:

Cai H, Peng F.

Journal:

J Fluoresc (2013): 213

In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog

Authors:

Tsytsarev V, Maslov KI, Yao J, Parameswar AR, Demchenko AV, Wang LV.

Journal:

J Neurosci Methods (2012): 136

Spectral properties

Excitation (nm)	467
Emission (nm)	538

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC filter
Emission	FITC filter
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 3, 2026

Component A: 2-NBDG (10 mg/mL)	1 vial (100 μL)
Component B: Assay Buffer I	1 bottle (50 mL)
Component C: Assay Buffer II	1 bottle (20 mL)