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Cell Meter™ Autophagy Fluorescence Imaging Kit
Autophagy is one of the major pathways for degradation of intracellular macromolecules in animal cells. The process of autophagy involves the sequestration of cytoplasmic materials and intracellular organelles in a membrane-bounded vacuole called autophagosome, the fusion of the autophagosome with lysosomes, and the subsequent degradation of sequestered materials. Cell Meter™ autophagy fluorescence imaging kit employs Autophagy Super Blue™ as a specific autophagosome marker to analyze the activity of autophagy. The assay is optimized for direct detection of autophagy in both detached and attached cells. The kit provides all the essential components for the assay protocol. The Cell Meter™ autophagy fluorescence imaging kit is optimized for fluorescence microscope. It gives much higher selectivity than other commercially available autophagy probes.
Autophagy Super Blue™ labeled vesicles were induced by starvation in HeLa cells. HeLa cells were incubated in a regular DMEM medium (Left: Control) or in 1X HBSS buffer with 5% serum (Right: Autophagy Treatment) for 16 hours. Both control and treated cells were incubated with Autophagy Super Blue™ working solution for 20 minutes in a 37 °C, 5% CO2 incubator, and washed 3 times with wash buffer. Cells were imaged immediately under a fluorescence microscope with a DAPI channel (blue). Cell nuclei were stained with Nuclear Green™ LCS1 (green).
Autophagy Super Blue™ labeled vesicles were induced by starvation in HeLa cells. HeLa cells were incubated in a regular DMEM medium (Left: Control) or in 1X HBSS buffer with 5% serum (Right: Autophagy Treatment) for 16 hours. Both control and treated cells were incubated with Autophagy Super Blue™ working solution for 20 minutes in a 37 °C, 5% CO2 incubator, and washed 3 times with wash buffer. Cells were imaged immediately under a fluorescence microscope with a DAPI channel (blue). Cell nuclei were stained with Nuclear Green™ LCS1 (green).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
23001200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationDAPI filter
EmissionDAPI filter
Recommended plateBlack wall/clear bottom
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Page updated on October 7, 2025