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Cell Meter™ Cell Viability Assay Kit
Green Fluorescence
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This kit uses the non-fluorescent calcein AM that becomes strongly fluorescent upon entering into live cells. Calcein AM is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent calcein AM by intracellular esterases generates the strongly fluorescent hydrophilic calcein that is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells, and thus directly related to the fluorescence intensity of calcein generated from the esterase-catalyzed hydrolysis of calcein AM. Cells grown in black-walled plates can be stained and quantified in less than two hours. The assay is more robust than the tetrazolium salt or Alarmar Blue™-based assays. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. Using 100 uL of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 500 assays. Using 25 uL of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 2000 assays.
CHO-K1 cell number response was measured with Cell Meter™ Cell Viability Assay Kit. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of dye-loading solution for 1 hour at 37°C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm with NOVOstar instrument (from BMG Labtech). The fluorescence intensity was linear (R square = 1) to the cell number as indicated.
CHO-K1 cell number response was measured with Cell Meter™ Cell Viability Assay Kit. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of dye-loading solution for 1 hour at 37°C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm with NOVOstar instrument (from BMG Labtech). The fluorescence intensity was linear (R square = 1) to the cell number as indicated.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
22786500 Tests
Price
 
Spectral properties
Excitation (nm)494
Emission (nm)514
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Brochure: Cell Viability & Proliferation
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Page updated on October 30, 2025