logo
Products
Services
Resources
Selection Guides
About
Cell Meter™ Cell Viability Assay Kit
Green Fluorescence
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This kit uses the non-fluorescent calcein AM that becomes strongly fluorescent upon entering into live cells. Calcein AM is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the non-fluorescent calcein AM by intracellular esterases generates the strongly fluorescent hydrophilic calcein that is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells, and thus directly related to the fluorescence intensity of calcein generated from the esterase-catalyzed hydrolysis of calcein AM. Cells grown in black-walled plates can be stained and quantified in less than two hours. The assay is more robust than the tetrazolium salt or Alarmar Blue™-based assays. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. Using 100 uL of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 500 assays. Using 25 uL of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 2000 assays.
CHO-K1 cell number response was measured with Cell Meter™ Cell Viability Assay Kit. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of dye-loading solution for 1 hour at 37°C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm with NOVOstar instrument (from BMG Labtech). The fluorescence intensity was linear (R square = 1) to the cell number as indicated.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
22786500 Tests
Price
 
Spectral properties
Excitation (nm)494
Emission (nm)514
Usage and storage
Intended useResearch Use Only (RUO)
Instrument settings
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on July 17, 2026