Name: Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit
Brand: AAT Bioquest
SKU: 22906
Price: 355 USD
Availability: InStock

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit

Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS). This process can lead to not only disruption of the cellular membrane integrity, but also inactivation of membrane-bound receptors. It is one of the main causes of free radical-mediated damages in cells. Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit provides a sensitive method for monitoring lipid peroxidation. The kit uses our sensitive ratiometric lipid peroxidation sensor, Lipoxite™ R590/G520 that changes its red fluorescence from red to green upon peroxidation by ROS in cells, this peroxidation-dependent shift enables the ratiometric measurement of lipid peroxidation. Our kit includes H2O2 as a positive control treatment to induce lipid peroxidation.

HeLa cells were stained with 1X Lipoxite™ R590/G525 for 30 mins in complete growth medium at 37°C. For H<sub>2</sub>O<sub>2</sub> treatment, approximately 250 µM of H<sub>2</sub>O<sub>2</sub> were added to the cells and incubated for 30 mins. The cells were then incubated with 1X Lipoxite™ R590/G525, and stained with Hoechst 33342 during the last 10 mins of incubation. The cells were washed 3 times with HHBS and imaged with a Keyence fluorescent microscope. With H<sub>2</sub>O<sub>2</sub> treatment, a clear shift of fluorescence signal of red to green was observed.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22906	200 Tests	Price

Citations

View all 19 citations: Citation Explorer

PDT-Enhanced Ferroptosis by a Polymer Nanoparticle with pH-Activated Singlet Oxygen Generation and Superb Biocompatibility for Cancer Therapy

Authors:

Li, Jing and Li, Junhua and Pu, Yuji and Li, Sai and Gao, Wenxia and He, Bin

Journal:

Biomacromolecules (2021): 1167--1176

Using fluorescence-activated flow cytometry to determine reactive oxygen species formation and membrane lipid peroxidation in viable boar spermatozoa

Authors:

Guthrie, H. D., Welch, G. R.

Journal:

Methods Mol Biol (2010): 163-71

Use of fluorescence-activated flow cytometry to determine membrane lipid peroxidation during hypothermic liquid storage and freeze-thawing of viable boar sperm loaded with 4, 4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic

Authors:

Guthrie, H. D., Welch, G. R.

Journal:

J Anim Sci (2007): 1402-11

Fluorescence microplate reader measurement of tissue susceptibility to lipid peroxidation

Authors:

Zhang, J. G., Fariss, M. W.

Journal:

Curr Protoc Toxicol (2004): Unit17 3

Response to ozone in two lettuce varieties on chlorophyll a fluorescence, photosynthetic pigments and lipid peroxidation

Authors:

Calatayud, A., Barreno, E.

Journal:

Plant Physiol Biochem (2004): 549-55

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm, 575/26 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	490 nm (FITC) and 545 nm (TRITC)
Emission	530 nm (FITC) and 600 nm (TRITC)
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC and TRITC channels

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Page updated on July 16, 2026

Component A: Lipoxite™ R590/G525 (500X)	1 vial (50 uL)
Component B: HHBS	1 bottle (50 mL)
Component C: 3% H2O2 (4000X, ~ 1M)	1 vial (100 μL)