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Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit
Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS). This process can lead to not only disruption of the cellular membrane integrity, but also inactivation of membrane-bound receptors. It is one of the main causes of free radical-mediated damages in cells. Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit provides a sensitive method for monitoring lipid peroxidation. The kit uses our sensitive ratiometric lipid peroxidation sensor, Lipoxite™ R590/G520 that changes its red fluorescence from red to green upon peroxidation by ROS in cells, this peroxidation-dependent shift enables the ratiometric measurement of lipid peroxidation. Our kit includes H2O2 as a positive control treatment to induce lipid peroxidation.
HeLa cells were stained with 1X Lipoxite&trade; R590/G525 for 30 mins in complete growth medium at 37&deg;C. For H<sub>2</sub>O<sub>2</sub> treatment, approximately 250 &micro;M of H<sub>2</sub>O<sub>2</sub> were added to the cells and incubated for 30 mins. The cells were then incubated with 1X Lipoxite&trade; R590/G525, and stained with Hoechst 33342 during the last 10 mins of incubation. The cells were washed 3 times with HHBS and imaged with a Keyence fluorescent microscope. With H<sub>2</sub>O<sub>2</sub> treatment, a clear shift of fluorescence signal of red to green was observed.
HeLa cells were stained with 1X Lipoxite&trade; R590/G525 for 30 mins in complete growth medium at 37&deg;C. For H<sub>2</sub>O<sub>2</sub> treatment, approximately 250 &micro;M of H<sub>2</sub>O<sub>2</sub> were added to the cells and incubated for 30 mins. The cells were then incubated with 1X Lipoxite&trade; R590/G525, and stained with Hoechst 33342 during the last 10 mins of incubation. The cells were washed 3 times with HHBS and imaged with a Keyence fluorescent microscope. With H<sub>2</sub>O<sub>2</sub> treatment, a clear shift of fluorescence signal of red to green was observed.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22906200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission530/30 nm, 575/26 nm filter
Instrument specification(s)FITC channel
Fluorescence microscope
Excitation490 nm (FITC) and 545 nm (TRITC)
Emission530 nm (FITC) and 600 nm (TRITC)
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC and TRITC channels
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Page updated on October 13, 2025