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Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit
NIR Fluorescence Optimized for Flow Cytometry
Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes. Altered NO production is implicated in various immunological, cardiovascular, neurodegenerative and inflammatory diseases. As a free radical, NO is rapidly oxidized and there is relatively low concentrations of NO existing in vivo. It has been challenging to detect and understand the role of NO in biological systems. Cell Meter™ Fluorimetric Intracellular Nitric Oxide Assay Kits provide sensitive tools to monitor intracellular NO level in live cells. Nitrixyte™ probes are developed and used in our assay kits as an excellent replacement for DAF-2 for the detection and imaging of free NO in cells. Compared to the commonly used DAF-2 probe, Nitrixyte™ probes have better photostability and enhanced cell permeability. This particular kit uses Nitrixyte™ NIR that can react with NO to generate strong near-infrared (NIR) fluorescence signal. Nitrixyte™ NIR can be readily loaded into live cells, and its fluorescence signal can be conveniently monitored using the filter set of Cy5® or APC. This kit is optimized for flow cytometry applications.
Detection of exogenous nitric oxide (NO) in Jurkat cells upon DEA NONOate treatment (NO donor) using Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit. Cells were incubated with Nitrixyte™ NIR for 30 minutes, and further treated with or without 1 mM DEA NONOate in Assay Buffer for 60 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in APC channel.
Detection of exogenous nitric oxide (NO) in Jurkat cells upon DEA NONOate treatment (NO donor) using Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit. Cells were incubated with Nitrixyte™ NIR for 30 minutes, and further treated with or without 1 mM DEA NONOate in Assay Buffer for 60 minutes. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in APC channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16360100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
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Page updated on October 8, 2025