Name: Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*
Brand: AAT Bioquest
SKU: 16315
Price: 406 USD
Availability: InStock

Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit

Green Fluorescence

Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent. Peroxynitrite is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can cause damages to a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Meanwhile, peroxynitrite can also have protective activities in vivo by contributing to host-defense responses against invading pathogens. Therefore, peroxynitrite is an essential biological oxidant involved in a board range of physiological and pathological processes. Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and understand the role of peroxynitrite in biological systems. AAT Bioquest's DAX-J2™ PON Green has been developed to address this unmet need. It provides a sensitive tool to monitor ONOO- level in living cells. AAT Bioquest's DAX-J2™ PON Green specifically reacts with intercellular ONOO- to generate a bright green fluorescent product. It can be used in fluorescence imaging, flow cytometry and fluorescence microplate reader-based assays.

Fluorescence images of intracellular peroxynitrite in RAW 264.7 macrophage cells using Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit (Cat#16315). Raw 264.7 cells at 100,000 cells/well/100 µL were seeded overnight in a Costar black wall/clear bottom 96-well plate. SIN-1 Treatment: Cells were co-incubated with DAX-J2™ PON Green and 100 µM SIN-1 at 37 °C for 1 hour. Untreated control: The RAW 264.7 cells were incubated with DAX-J2™ PON Green without SIN-1 treatment. The fluorescence signals were measured using a fluorescence microscope with a FITC filter

Protocol

SDS

Catalog	Size	Price	Quantity
16315	100 Tests	Price

Citations

View all 11 citations: Citation Explorer

Self-targeted nanosystem for enhanced chemodynamic cancer therapy

Authors:

Asif, Kanwal and Rahman, Md Mahbubur and Canzonieri, Vincenzo and Caligiuri, Isabella and Rizzolio, Flavio and Adeel, Muhammad

Journal:

Biomaterials Science (2025)

Hybridized and engineered microbe for catalytic generation of peroxynitrite and cancer immunotherapy under sonopiezo initiation

Authors:

Wang, Liping and Ji, Penghao and Yu, Jiadie and Qiu, Shuwen and An, Bolin and Huo, Minfeng and Shi, Jianlin

Journal:

Science Advances (2024): eadp7540

Quantum Molecular Resonance Inhibits NLRP3 Inflammasome/Nitrosative Stress and Promotes M1 to M2 Macrophage Polarization: Potential Therapeutic Effect in Osteoarthritis Model In Vitro

Authors:

Paolucci, Teresa and Pino, Vanessa and Elsallabi, Osama and Gallorini, Marialucia and Pozzato, Gianantonio and Pozzato, Alessandro and Lanuti, Paola and Reis, Victor Machado and Pesce, Mirko and Pantalone, Andrea and others,

Journal:

Antioxidants (2023): 1358

Silver Nitroprusside as an Efficient Chemodynamic Therapeutic Agent and a Peroxynitrite nanogenerator for Targeted Cancer Therapy

Authors:

Asif, Kanwal and Adeel, Muhammad and Rahman, Md Mahbubur and Sfriso, Andrea Augusto and Bartoletti, Michele and Canzonieri, Vincenzo and Rizzolio, Flavio and Caligiuri, Isabella

Journal:

Journal of Advanced Research (2023)

Triterpenoids and ultrasound dual-catalytic nanoreactor ignites long-lived hypertoxic reactive species storm for deep tumor treatment

Authors:

Li, Ziying and Xie, Huanzhang and Shi, Huifang and Li, Dongmiao and Zhang, Zizhong and Chen, Haijun and Gao, Yu

Journal:

Chemical Engineering Journal (2023): 139938

References

View all 10 references: Citation Explorer

Imaging of nucleolar RNA in living cells using a highly photostable deep-red fluorescent probe

Authors:

Zhou B, Liu W, Zhang H, Wu J, Liu S, Xu H, Wang P.

Journal:

Biosens Bioelectron (2015): 189

RNA and DNA binding of inert oligonuclear ruthenium(II) complexes in live eukaryotic cells

Authors:

Li X, Gorle AK, Ainsworth TD, Heimann K, Woodward CE, Collins JG, Keene FR.

Journal:

Dalton Trans (2015): 3594

Low molecular weight fluorescent probes with good photostability for imaging RNA-rich nucleolus and RNA in cytoplasm in living cells

Authors:

Song G, Sun Y, Liu Y, Wang X, Chen M, Miao F, Zhang W, Yu X, Jin J.

Journal:

Biomaterials (2014): 2103

Luminescence of [Ru(bpy)2(dppz)]2+ bound to RNA mismatches

Authors:

McConnell AJ, Song H, Barton JK.

Journal:

Inorg Chem (2013): 10131

Co-aggregation of RNA binding proteins in ALS spinal motor neurons: evidence of a common pathogenic mechanism

Authors:

Keller BA, Volkening K, Droppelmann CA, Ang LC, Rademakers R, Strong MJ.

Journal:

Acta Neuropathol (2012): 733

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	490 nm
Emission	530 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC Filter Set

Fluorescence microplate reader
Excitation	490 nm
Emission	530 nm
Cutoff	515 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Reactive Oxygen Species (ROS) Detection
Flyer: Reactive Oxygen Species (ROS)

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 30, 2025

Component A: DAX-J2™ PON Green	1 vial
Component B: Assay Buffer	1 vial (1 mL/vial)
Component C: DMSO	1 vial (100 µL/vial)