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Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit
Green Fluorescence
Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent. Peroxynitrite is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can cause damages to a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Meanwhile, peroxynitrite can also have protective activities in vivo by contributing to host-defense responses against invading pathogens. Therefore, peroxynitrite is an essential biological oxidant involved in a board range of physiological and pathological processes. Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and understand the role of peroxynitrite in biological systems. AAT Bioquest's DAX-J2™ PON Green has been developed to address this unmet need. It provides a sensitive tool to monitor ONOO- level in living cells. AAT Bioquest's DAX-J2™ PON Green specifically reacts with intercellular ONOO- to generate a bright green fluorescent product. It can be used in fluorescence imaging, flow cytometry and fluorescence microplate reader-based assays.
Fluorescence images of intracellular peroxynitrite in RAW 264.7 macrophage cells using Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit (Cat#16315). Raw 264.7 cells at 100,000 cells/well/100 µL were seeded overnight in a Costar black wall/clear bottom 96-well plate. SIN-1 Treatment: Cells were co-incubated with DAX-J2™ PON Green and 100 µM SIN-1 at 37 °C for 1 hour. Untreated control: The RAW 264.7 cells were incubated with DAX-J2™ PON Green without SIN-1 treatment. The fluorescence signals were measured using a fluorescence microscope with a FITC filter
Fluorescence images of intracellular peroxynitrite in RAW 264.7 macrophage cells using Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit (Cat#16315). Raw 264.7 cells at 100,000 cells/well/100 µL were seeded overnight in a Costar black wall/clear bottom 96-well plate. SIN-1 Treatment: Cells were co-incubated with DAX-J2™ PON Green and 100 µM SIN-1 at 37 °C for 1 hour. Untreated control: The RAW 264.7 cells were incubated with DAX-J2™ PON Green without SIN-1 treatment. The fluorescence signals were measured using a fluorescence microscope with a FITC filter
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16315100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
Excitation490 nm
Emission530 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC Filter Set
Fluorescence microplate reader
Excitation490 nm
Emission530 nm
Cutoff515 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on October 8, 2025