Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
Platform
Absorbance microplate reader
Absorbance | 695 nm |
Recommended plate | Clear bottom |
Components
Example protocol
AT A GLANCE
- Prepare and add MDA standards and/or test samples (50 µL)
- Prepare and add MDA Blue™ (10 µL)
- Incubate at room temperature for 10 - 30 minutes
- Add reaction solution (40 µL)
- Monitor OD increase at 695 nm
Thaw all the kit components at room temperature before starting the experiment.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 100 µL of ddH2O into MDA Standard (Component C) and mix them well. Note: The unused MDA stock solution should be stored at -20 °C in single use aliquots.
PREPARATION OF STANDARD SOLUTIONS
https://www.aatbio.com/tools/serial-dilution/15991
SAMPLE EXPERIMENTAL PROTOCOL
Table 1. Layout of MDA standards and test samples in a clear bottom 96-well microplate. MDA = MDA standard (MDA1-MDA7= 1000 to 50 µM); BL = blank control; TS = test sample.
BL | BL | TS | TS |
MDA1 | MDA1 | ... | ... |
MDA2 | MDA2 | ... | ... |
MDA3 | MDA3 | ||
MDA4 | MDA4 | ||
MDA5 | MDA5 | ||
MDA6 | MDA6 | ||
MDA7 | MDA7 |
Table 2. Reagent composition for each well.
Well | Volume | Reagent |
MDA1-MDA7 | 50 µL | Serial Dilution (1000 to 50 µM) |
BL | 50 µL | Dilution Buffer (Component B) |
TS | 50 µL | Test Sample |
- Add 50 µL of MDA standard, blank control, and test samples to clear bottom 96-well microplate (As shown in Table 1 and Table 2).
Add 10 µL/well of MDA Blue™ (Component A) into each well of MDA standard, blank control and test samples. Note: For a 384-well plate, add 25 µL of sample, 5 µL of MDA Blue™ solution into each well. Note: Please aliquot Component A into single use size and store unused at -20 oC and avoid light.
- Incubate the reaction at room temperature for 10 - 30 minutes, protected from light.
Add 40 µL of Reaction Solution (Component D) to make the total assay volume of 100 µL/well. Note: For a 384-well plate, add 20 µL of Reaction Solution (Component D) to make the total assay volume of 50 µL/well.
- Monitor absorbance increase with an absorbance plate reader with path-check correction at OD of 695~700 nm.
Images
References
Authors: Tsikas D., undefined
Journal: Anal Biochem (2016)
Authors: Steppeler C, Haugen JE, Rodbotten R, Kirkhus B.
Journal: J Agric Food Chem (2016): 487
Authors: Tsikas D, Rothmann S, Schneider JY, Suchy MT, Trettin A, Modun D, Stuke N, Maassen N, Frolich JC.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2016): 95
Authors: Spirl, undefined and eli AL, Deminice R, Jordao AA.
Journal: Int J Sports Med (2014): 14
Authors: Moselhy HF, Reid RG, Yousef S, Boyle SP.
Journal: J Lipid Res (2013): 852
Authors: Puntel RL, Roos DH, Grotto D, Garcia SC, Nogueira CW, Rocha JB.
Journal: Life Sci (2007): 51
Authors: Lykkesfeldt J., undefined
Journal: Clin Chim Acta (2007): 50
Authors: Grotto D, Santa Maria LD, Boeira S, Valentini J, Charao MF, Moro AM, Nascimento PC, Pomblum VJ, Garcia SC.
Journal: J Pharm Biomed Anal (2007): 619
Authors: Faschinger C, Schmut O, Wachswender C, Mossbock G.
Journal: Ophthalmologe (2006): 953
Authors: Kudolo GB, Delaney D, Blodgett J.
Journal: Diabetes Res Clin Pract (2005): 29
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