Name: Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit
Brand: AAT Bioquest
SKU: 15991
Price: 465 USD
Availability: InStock

Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit

Lipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol. Malondialdehyde (MDA) is one of the most commonly used biomarkers for lipid peroxidation. Measurement of MDA has historically relied on a reaction with thiobarbituric acid (TBA) to generate a product that can be measured colorimetrically or fluorimetrically. However, TBA assay has quite a few limitations. (1). The reaction is not specific to MDA. (2). TBA-MDA reaction need be run under acidic conditions. (3). The assays need be run under high temperature, commonly at 90-100 ºC. The commercial TBA-based MDA assays are extremely tedious to run due to these limitations. This Cell Meter™ Colorimetric Lipid Peroxidation (MDA) Quantitation Kit offers the most rapid and convenient method to measure MDA without the TBARS heating steps. MDA Blue™ reacts with MDA to generate a blue color product which is measured at 695 nm with absorbance microplate reader. This assay is very fast and specific to MDA with little interference from other aldehydes.

MDA dose response was measured with Amplite® Colorimetric Lipid Peroxidation (MDA) Quantitation Kit on a 96-well clear bottom microplate using a SpectraMax microplate reader (Molecular Devices). (Pathcheck on (Left image); Pathcheck off (Right image))

Catalog	Size	Price	Quantity
15991	200 tests	Price

References

View all 21 references: Citation Explorer

Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges.

Authors:

Tsikas D., undefined

Journal:

Anal Biochem (2016)

Formation of Malondialdehyde, 4-Hydroxynonenal, and 4-Hydroxyhexenal during in Vitro Digestion of Cooked Beef, Pork, Chicken, and Salmon

Authors:

Steppeler C, Haugen JE, Rodbotten R, Kirkhus B.

Journal:

J Agric Food Chem (2016): 487

Development, validation and biomedical applications of stable-isotope dilution GC-MS and GC-MS/MS techniques for circulating malondialdehyde (MDA) after pentafluorobenzyl bromide derivatization: MDA as a biomarker of oxidative stress and its relation to 15

Authors:

Tsikas D, Rothmann S, Schneider JY, Suchy MT, Trettin A, Modun D, Stuke N, Maassen N, Frolich JC.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2016): 95

Plasma malondialdehyde as biomarker of lipid peroxidation: effects of acute exercise

Authors:

Spirl, undefined and eli AL, Deminice R, Jordao AA.

Journal:

Int J Sports Med (2014): 14

A specific, accurate, and sensitive measure of total plasma malondialdehyde by HPLC

Authors:

Moselhy HF, Reid RG, Yousef S, Boyle SP.

Journal:

J Lipid Res (2013): 852

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	695 nm
Recommended plate	Clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis

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Page updated on October 20, 2025

Component A: MDA Blue™	1 vial
Component B: Dilution Buffer	1 bottle (10 mL)
Component C: MDA Standard	1 vial (lyophilized)
Component D: Reaction Solution	1 bottle (10 mL)