Name: Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry*
Brand: AAT Bioquest
SKU: 22810
Price: 232 USD
Availability: InStock

Cell Meter™ Intracellular GSH Assay Kit

Optimized for Flow Cytometry

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the depleted antioxidant reduced glutathione (GSH). GSH is involved in many cellular processes including the scavenging of free radicals, drug detoxification, cell signaling, and cell proliferation. The decrease in cellular GSH concentration is an early hallmark in the progression of cell death in response to different apoptotic stimuli in many cell types. Our Cell Meter™ Intracellular GSH Assay Kit provides all the essential components with an optimized assay method for the detection of apoptosis in cells with the decreased GSH. This fluorometric assay is based on the detection of the GSH in cells using our proprietary non-fluorescent Thiolite™ Green dye that becomes strongly fluorescent upon reacting with thiol. In normal cells, Thiolite™ Green accumulates primarily in cytosol, but it is partially translocated to mitochondria in apoptotic cells while Thiolite™ Green staining intensity decreases. Cells stained with Thiolite™ Green can be visualized by flow cytometry. The kit can be paired with other reagents, such as 7-AAD (Cat# 17501), propidium iodide (Cat# 17517) for multi-parametric study of cell viability and apoptosis. The kit is optimized for screening of apoptosis activators and inhibitors by flow cytometry.

The decrease in the fluorescence intensity of Thiolite™ Green with the addition of camptothecin in Jurkat cells. Jurkat cells were treated overnight without (blue line) or with 20 µM camptothecin (red line) in a 37 °C, 5% CO2 incubator, and then dye loaded with Thiolite™ Green for 30 minutes. The fluorescence intensity of Thiolite™ Green was measured with a FACSCalibur (Becton Dickinson) flow cytometer using the FL1 channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22810	100 Tests	Price

Citations

View all 7 citations: Citation Explorer

The Combination of Astragalus membranaceus and Angelica sinensis Inhibits Lung Cancer and Cachexia through Its Immunomodulatory Function

Authors:

Wu, Tsung-Han and Yeh, Kun-Yun and Wang, Chen-Hsu and Wang, Hang and Li, Tsung-Lin and Chan, Yi-Lin and Wu, Chang-Jer

Journal:

Journal of oncology (2019)

ROS production and glutathione response in keratinocytes after application of β-carotene and VIS/NIR irradiation

Authors:

Lohan, Silke B and Vitt, Kristina and Scholz, Patrik and Keck, Cornelia M and Meinke, Martina C

Journal:

Chemico-biological interactions (2017)

Osmotic stress is accompanied by protein glycation in Arabidopsis thaliana

Authors:

Paudel, Gagan and Bilova, Tatiana and Schmidt, Rico and Greifenhagen, Uta and Berger, Robert and Tarakhovskaya, Elena and Stöckhardt, Stefanie and Balcke, Gerd Ulrich and Humbeck, Klaus and Br, undefined and t, Wolfgang and others, undefined

Journal:

Journal of Experimental Botany (2016): 6283--6295

Systemic Induction of NO-, Redox-, and cGMP Signaling in the Pumpkin Extrafascicular Phloem upon Local Leaf Wounding

Authors:

Gaupels, Frank and Furch, Alex and ra CU , undefined and Zimmermann, Matthias R and Chen, Faxing and Kaever, Volkhard and Buhtz, Anja and Kehr, Julia and Sarioglu, Hakan and Kogel, Karl-Heinz and Durner, Jörg

Journal:

Frontiers in plant science (2016)

Prediction of intracellular metabolic states from extracellular metabolomic data

Authors:

Aurich, Maike K and Paglia, Giuseppe and Rolfsson, Ottar and Hrafnsdóttir, Sigrún and Magnúsdóttir, Manuela and Stefaniak, Magdalena M and Palsson, Bernhard O and Fleming, Ronan MT and Thiele, Ines

Journal:

Metabolomics (2015): 603--619

References

View all 48 references: Citation Explorer

Investigation of the spatial distribution of glutathione redox-balance in live cells by using Fluorescence Ratio Imaging Microscopy

Authors:

Maulucci G, Pani G, Labate V, Mele M, Panieri E, Papi M, Arcovito G, Galeotti T, De Spirito M.

Journal:

Biosens Bioelectron (2009): 682

Fast determination of glutathione by capillary electrophoresis with fluorescence detection using beta-cyclodextrin as modifier

Authors:

Zhang LY, Sun MX.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2009): 4051

Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection

Authors:

Wang Y, Xie Y, Bernier M, Wainer IW.

Journal:

J Chromatogr A (2009): 3533

Second-order calibration of excitation-emission matrix fluorescence spectra for determination of glutathione in human plasma

Authors:

Hemmateenejad B, Rezaei Z, Zaeri S.

Journal:

Talanta (2009): 648

A highly sensitive fluorescence probe for fast thiol-quantification assay of glutathione reductase

Authors:

Yi L, Li H, Sun L, Liu L, Zhang C, Xi Z.

Journal:

Angew Chem Int Ed Engl (2009): 4034

Spectral properties

Excitation (nm)	505
Emission (nm)	524

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 29, 2025

Component A: Thiolite™ Green	1 vial
Component B: Assay Buffer	1 bottle (100 mL)
Component C: DMSO	1 vial (500 µL)