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DABCYL-DBCO

Chemical structure for DABCYL-DBCO.
Chemical structure for DABCYL-DBCO.
Ordering information
Price ()
Catalog Number2010
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight527.63
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
527.63
DABCYL is one of the most common dark quenchers used to prepare a variety of FRET-based nucleic acid probes and protease substrates. A dark quencher is a substance that absorbs excitation energy from a fluorophore and dissipates the energy as heat. When a dark quencher and a fluorophore are close together, such as in a molecule or protein, the fluorophore's emission is suppressed. This effect can be used to study molecular geometry and motion. DABCYL-DBCO is a convenient building block for developing FRET probes via the DBCO-based click chemistry, one of the most well-known strain-promoted azide-alkyne cycloadditions (SPAAC).

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL-DBCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM189.527 µL947.634 µL1.895 mL9.476 mL18.953 mL
5 mM37.905 µL189.527 µL379.054 µL1.895 mL3.791 mL
10 mM18.953 µL94.763 µL189.527 µL947.634 µL1.895 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
DBCO-Cy355556915000010.1510.070.073
DBCO-Cy565167025000010.271, 0.420.020.03
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*-----0.516
Cy3B DBCO56057112000010.5810.0480.069

References


View all 50 references: Citation Explorer
A Cell Membrane-Targeting Self-Delivery Chimeric Peptide for Enhanced Photodynamic Therapy and In Situ Therapeutic Feedback.
Authors: Ma, Wen and Sha, Sui-Nan and Chen, Pei-Ling and Yu, Meng and Chen, Jian-Jun and Huang, Chao-Bo and Yu, Bin and Liu, Yun and Liu, Li-Han and Yu, Zhi-Qiang
Journal: Advanced healthcare materials (2020): e1901100
Polymeric fluorescent heparin as one-step FRET substrate of human heparanase.
Authors: Sistla, Jyothi C and Morla, Shravan and Alabbas, Al-Humaidi B and Kalathur, Ravi C and Sharon, Chetna and Patel, Bhaumik B and Desai, Umesh R
Journal: Carbohydrate polymers (2019): 385-391
FRET-Based Assays to Determine Calpain Activity.
Authors: McCartney, Christian-Scott E and Davies, Peter L
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 39-55
Array-based in situ fluorescence assay for profiling multiplex matrix metalloproteinases activities in tissue section.
Authors: Lei, Zhen and Jian, Minghong and Wei, Jia and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
Journal: Analytica chimica acta (2019): 112-118
The conversion of azo-quenchers to fluorophores.
Authors: Hofstetter, O and Hofstetter, H and Miron, T and Wilchek, M
Journal: Analytical biochemistry (2019): 113400
Development of a peptide substrate for detection of sunn pest damage in wheat flour.
Authors: Hançerlioğulları, Begüm Zeynep and Köksel, Hamit and Dudak, Fahriye Ceyda
Journal: Journal of the science of food and agriculture (2018): 5677-5682
A FRET-based dual-color evanescent wave optical fiber aptasensor for simultaneous fluorometric determination of aflatoxin M1 and ochratoxin A.
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Long, Feng
Journal: Mikrochimica acta (2018): 508
Uleine Disrupts Key Enzymatic and Non-Enzymatic Biomarkers that Leads to Alzheimer's Disease.
Authors: Seidl, Claudia and de Moraes Santos, Cid Aimbire and De Simone, Angela and Bartolini, Manuela and Weffort-Santos, Almeriane Maria and Andrisano, Vincenza
Journal: Current Alzheimer research (2017): 317-326
Highly specific C-C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging.
Authors: Li, Hua and Zhang, Deliang and Gao, Mengna and Huang, Lumei and Tang, Longguang and Li, Zijing and Chen, Xiaoyuan and Zhang, Xianzhong
Journal: Chemical science (2017): 2199-2203
Chemoenzymatic synthesis of fluorogenic phospholipids and evaluation in assays of phospholipases A, C and D.
Authors: Piel, Mathilde S and Peters, Günther H J and Brask, Jesper
Journal: Chemistry and physics of lipids (2017): 49-54