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Physical properties
Molecular weight527.63
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
DABCYL is one of the most common dark quenchers used to prepare a variety of FRET-based nucleic acid probes and protease substrates. A dark quencher is a substance that absorbs excitation energy from a fluorophore and dissipates the energy as heat. When a dark quencher and a fluorophore are close together, such as in a molecule or protein, the fluorophore's emission is suppressed. This effect can be used to study molecular geometry and motion. DABCYL-DBCO is a convenient building block for developing FRET probes via the DBCO-based click chemistry, one of the most well-known strain-promoted azide-alkyne cycloadditions (SPAAC).


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL-DBCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM189.527 µL947.634 µL1.895 mL9.476 mL18.953 mL
5 mM37.905 µL189.527 µL379.054 µL1.895 mL3.791 mL
10 mM18.953 µL94.763 µL189.527 µL947.634 µL1.895 mL

Molarity calculator

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Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
DBCO-Cy565167025000010.271, 0.420.020.03
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*-----0.516
Cy3B DBCO56057112000010.5810.0480.069



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