DABCYL-DBCO
| Overview |  SDSProtocol |
See also: Cell Structures and Organelles, Nucleus, DNA and RNA Quantitation, Physiological Probes, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), PCR Detection of Viral DNA/RNA
Molecular weight 527.63 |
DABCYL is one of the most common dark quenchers used to prepare a variety of FRET-based nucleic acid probes and protease substrates. A dark quencher is a substance that absorbs excitation energy from a fluorophore and dissipates the energy as heat. When a dark quencher and a fluorophore are close together, such as in a molecule or protein, the fluorophore's emission is suppressed. This effect can be used to study molecular geometry and motion. DABCYL-DBCO is a convenient building block for developing FRET probes via the DBCO-based click chemistry, one of the most well-known strain-promoted azide-alkyne cycloadditions (SPAAC).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL-DBCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 189.527 µL | 947.634 µL | 1.895 mL | 9.476 mL | 18.953 mL |
| 5 mM | 37.905 µL | 189.527 µL | 379.054 µL | 1.895 mL | 3.791 mL |
| 10 mM | 18.953 µL | 94.763 µL | 189.527 µL | 947.634 µL | 1.895 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| DBCO-Cy3 | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
| DBCO-Cy5 | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* | - | - | - | - | - | 0.516 |
| Cy3B DBCO | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
Images
Figure 1. Chemical structure for DABCYL-DBCO.
References
View all 50 references: Citation Explorer
A Cell Membrane-Targeting Self-Delivery Chimeric Peptide for Enhanced Photodynamic Therapy and In Situ Therapeutic Feedback.
Authors: Ma, Wen and Sha, Sui-Nan and Chen, Pei-Ling and Yu, Meng and Chen, Jian-Jun and Huang, Chao-Bo and Yu, Bin and Liu, Yun and Liu, Li-Han and Yu, Zhi-Qiang
Journal: Advanced healthcare materials (2020): e1901100
Authors: Ma, Wen and Sha, Sui-Nan and Chen, Pei-Ling and Yu, Meng and Chen, Jian-Jun and Huang, Chao-Bo and Yu, Bin and Liu, Yun and Liu, Li-Han and Yu, Zhi-Qiang
Journal: Advanced healthcare materials (2020): e1901100
Polymeric fluorescent heparin as one-step FRET substrate of human heparanase.
Authors: Sistla, Jyothi C and Morla, Shravan and Alabbas, Al-Humaidi B and Kalathur, Ravi C and Sharon, Chetna and Patel, Bhaumik B and Desai, Umesh R
Journal: Carbohydrate polymers (2019): 385-391
Authors: Sistla, Jyothi C and Morla, Shravan and Alabbas, Al-Humaidi B and Kalathur, Ravi C and Sharon, Chetna and Patel, Bhaumik B and Desai, Umesh R
Journal: Carbohydrate polymers (2019): 385-391
FRET-Based Assays to Determine Calpain Activity.
Authors: McCartney, Christian-Scott E and Davies, Peter L
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 39-55
Authors: McCartney, Christian-Scott E and Davies, Peter L
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 39-55
Array-based in situ fluorescence assay for profiling multiplex matrix metalloproteinases activities in tissue section.
Authors: Lei, Zhen and Jian, Minghong and Wei, Jia and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
Journal: Analytica chimica acta (2019): 112-118
Authors: Lei, Zhen and Jian, Minghong and Wei, Jia and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
Journal: Analytica chimica acta (2019): 112-118
The conversion of azo-quenchers to fluorophores.
Authors: Hofstetter, O and Hofstetter, H and Miron, T and Wilchek, M
Journal: Analytical biochemistry (2019): 113400
Authors: Hofstetter, O and Hofstetter, H and Miron, T and Wilchek, M
Journal: Analytical biochemistry (2019): 113400
Development of a peptide substrate for detection of sunn pest damage in wheat flour.
Authors: Hançerlioğulları, Begüm Zeynep and Köksel, Hamit and Dudak, Fahriye Ceyda
Journal: Journal of the science of food and agriculture (2018): 5677-5682
Authors: Hançerlioğulları, Begüm Zeynep and Köksel, Hamit and Dudak, Fahriye Ceyda
Journal: Journal of the science of food and agriculture (2018): 5677-5682
A FRET-based dual-color evanescent wave optical fiber aptasensor for simultaneous fluorometric determination of aflatoxin M1 and ochratoxin A.
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Long, Feng
Journal: Mikrochimica acta (2018): 508
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Long, Feng
Journal: Mikrochimica acta (2018): 508
Uleine Disrupts Key Enzymatic and Non-Enzymatic Biomarkers that Leads to Alzheimer's Disease.
Authors: Seidl, Claudia and de Moraes Santos, Cid Aimbire and De Simone, Angela and Bartolini, Manuela and Weffort-Santos, Almeriane Maria and Andrisano, Vincenza
Journal: Current Alzheimer research (2017): 317-326
Authors: Seidl, Claudia and de Moraes Santos, Cid Aimbire and De Simone, Angela and Bartolini, Manuela and Weffort-Santos, Almeriane Maria and Andrisano, Vincenza
Journal: Current Alzheimer research (2017): 317-326
Highly specific C-C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging.
Authors: Li, Hua and Zhang, Deliang and Gao, Mengna and Huang, Lumei and Tang, Longguang and Li, Zijing and Chen, Xiaoyuan and Zhang, Xianzhong
Journal: Chemical science (2017): 2199-2203
Authors: Li, Hua and Zhang, Deliang and Gao, Mengna and Huang, Lumei and Tang, Longguang and Li, Zijing and Chen, Xiaoyuan and Zhang, Xianzhong
Journal: Chemical science (2017): 2199-2203
Chemoenzymatic synthesis of fluorogenic phospholipids and evaluation in assays of phospholipases A, C and D.
Authors: Piel, Mathilde S and Peters, Günther H J and Brask, Jesper
Journal: Chemistry and physics of lipids (2017): 49-54
Authors: Piel, Mathilde S and Peters, Günther H J and Brask, Jesper
Journal: Chemistry and physics of lipids (2017): 49-54