DAX-J2™ Red

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	608.77
Solvent	DMSO

Spectral properties

Excitation (nm)	587
Emission (nm)	609

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Alternative formats

Overview SDS Protocol

Molecular weight

608.77

Excitation (nm)

587

Emission (nm)

609

DAX-J2™ Red is a new nitric oxide (NO) sensor recently developed by AAT Bioquest. It is a non-fluorescent cell permeable reagent that can measure free NO and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the blocking groups on the DAX-J2 reagent are released to generate the highly fluorescent product upon NO oxidation. DAX-J2 fluorescent product can be detected using the filter set of Texas Red® that is equipped with most of flow cytometers and fluorescence microscope. DAX-J2 Red has distinct advantages for NO detection than the popular DAF-2 NO probe: 1). It does not require esterase activity for NO detection. DAF-2 requires intracellular esterases to cleave its acetate groups for detecting NO activity. This esterase dependence often complicates the NO detection since esterase activities are affected by cell health and many other factors. 2). DAX-2 product exhibits pH-independent fluorescence while DAF-2 has its fluorescence highly affected by pH. 3). DAX-J2 Red fluorescent product is much more photostable than DAF-2 product. 4). It is more sensitive for detecting NO than DAF-2. 5). It can be used with GFP cell lines or with the applications that use FITC-labeled antibodies for multicolor cell analysis.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DAX-J2™ Red to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	164.266 µL	821.328 µL	1.643 mL	8.213 mL	16.427 mL
5 mM	32.853 µL	164.266 µL	328.531 µL	1.643 mL	3.285 mL
10 mM	16.427 µL	82.133 µL	164.266 µL	821.328 µL	1.643 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	587
Emission (nm)	609

Product Family

Name	Excitation (nm)	Emission (nm)
DAX-J2™ Orange	552	575

Images

Figure 1. Fluorescence response of DAX-J2^TM Red (2 uM) to different reactive oxygen species (1 mM) in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 570/610 nm.

Citations

View all 7 citations: Citation Explorer

Low-dose daylight exposure induces nitric oxide release and maintains cell viability in vitro
Authors: Hazell, Gareth and Khazova, Marina
Journal: (2023)

Substance P-induced activation of presynaptic NK1 receptors suppresses EPSCs via nitric oxide synthesis in the rat insular cortex
Authors: 松村幸恵,
Journal: (2021)

Presynaptic NK1 Receptor Activation by Substance P Suppresses EPSCs via Nitric Oxide Synthesis in the Rat Insular Cortex
Authors: Matsumura, Sachie and Yamamoto, Kiyofumi and Nakaya, Yuka and O'Hashi, Kazunori and Kaneko, Keisuke and Takei, Hiroki and Tsuda, Hiromasa and Shirakawa, Tetsuo and Kobayashi, Masayuki
Journal: Neuroscience (2021): 151--164

One-step preparation of the engineered titanium implant by rationally designed linear fusion peptides with spacer-dependent antimicrobial, anti-inflammatory and osteogenic activities
Authors: Xin, Haoqian and Chen, Junjian and Li, Tianjie and Hu, Guansong and Fang, Zhou and Zhou, Haiyan and Guo, Kunzhong and Wang, Lin and Wang, Yingjun
Journal: Chemical Engineering Journal (2021): 130380

Aspirin eugenol ester attenuates oxidative injury of vascular endothelial cells by regulating NOS and Nrf2 signaling pathways
Authors: Huang, Mei-Zhou and Yang, Ya-Jun and Liu, Xi-Wang and Qin, Zhe and Li, Jian-Yong
Journal: British Journal of Pharmacology (2019)

Fluorescent real-time quantitative measurements of intracellular peroxynitrite generation and inhibition
Authors: Luo, Zhen and Zhao, Qin and Liu, Jixiang and Liao, Jinfang and Peng, Ruogu and Xi, Yunting and Diwu, Zhenjun
Journal: Analytical biochemistry (2017): 44--48

Inducible Nitric Oxide Synthase (iNOS) Is a Novel Negative Regulator of Hematopoietic Stem/Progenitor Cell Trafficking
Authors: Adamiak, Mateusz and Abdelbaset-Ismail, Ahmed and Moore, Joseph B and Zhao, J and Abdel-Latif, Ahmed and Wysoczynski, Marcin and Ratajczak, Mariusz Z
Journal: Stem Cell Reviews and Reports (2016): 1--12

References

View all 139 references: Citation Explorer

Pitfalls and limitations in using 4,5-diaminofluorescein for evaluating the influence of polyphenols on nitric oxide release from endothelial cells
Authors: Uhlenhut K, Hogger P.
Journal: Free Radic Biol Med (2012): 2266

Effects of moderate electrical stimulation on reactive species production by primary rat skeletal muscle cells: cross talk between superoxide and nitric oxide production
Authors: Lambertucci RH, Silveira Ldos R, Hirabara SM, Curi R, Sweeney G, Pithon-Curi TC.
Journal: J Cell Physiol (2012): 2511

Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate
Authors: Zhou X, He P.
Journal: Am J Physiol Heart Circ Physiol (2011): H108

Aging negatively affects estrogens-mediated effects on nitric oxide bioavailability by shifting ERalpha/ERbeta balance in female mice
Authors: Novensa L, Novella S, Medina P, Segarra G, Castillo N, Heras M, Hermenegildo C, Dantas AP.
Journal: PLoS One (2011): e25335

Temporal and spatial correlation of platelet-activating factor-induced increases in endothelial [Ca(2)(+)]i, nitric oxide, and gap formation in intact venules
Authors: Zhou X, He P.
Journal: Am J Physiol Heart Circ Physiol (2011): H1788

Polyamines, polyamine oxidases and nitric oxide in development, abiotic and biotic stresses
Authors: Wimalasekera R, Tebartz F, Scherer GF.
Journal: Plant Sci (2011): 593

Rapid upregulation of cytoprotective nitric oxide in breast tumor cells subjected to a photodynamic therapy-like oxidative challenge
Authors: Bhowmick R, Girotti AW.
Journal: Photochem Photobiol (2011): 378

Sleep deprivation triggers inducible nitric oxide-dependent nitric oxide production in wake-active basal forebrain neurons
Authors: Kalinchuk AV, McCarley RW, Porkka-Heiskanen T, Basheer R.
Journal: J Neurosci (2010): 13254

Production and scavenging of nitric oxide by barley root mitochondria
Authors: Gupta KJ, Kaiser WM.
Journal: Plant Cell Physiol (2010): 576

Production of Nitric Oxide within the Aplysia Californica Nervous System
Authors: Ye X, Xie F, Romanova EV, Rubakhin SS, Sweedler JV.
Journal: ACS Chem Neurosci (2010): 182