AAT Bioquest

DAX-J2™ Red

Fluorescence response of DAX-J2<sup>TM</sup> Red (2 uM) to different reactive oxygen species (1 mM) in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 570/610 nm.
Fluorescence response of DAX-J2<sup>TM</sup> Red (2 uM) to different reactive oxygen species (1 mM) in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 570/610 nm.
Fluorescence response of DAX-J2<sup>TM</sup> Red (2 uM) to different reactive oxygen species (1 mM) in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 570/610 nm.
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Physical properties
Molecular weight608.77
Spectral properties
Excitation (nm)587
Emission (nm)609
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
DAX-J2™ Orange
Related products
DAX-J2™ Ratio 580/460


Molecular weight
Excitation (nm)
Emission (nm)
DAX-J2™ Red is a new nitric oxide (NO) sensor recently developed by AAT Bioquest. It is a non-fluorescent cell permeable reagent that can measure free NO and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the blocking groups on the DAX-J2 reagent are released to generate the highly fluorescent product upon NO oxidation. DAX-J2 fluorescent product can be detected using the filter set of Texas Red® that is equipped with most of flow cytometers and fluorescence microscope. DAX-J2 Red has distinct advantages for NO detection than the popular DAF-2 NO probe: 1). It does not require esterase activity for NO detection. DAF-2 requires intracellular esterases to cleave its acetate groups for detecting NO activity. This esterase dependence often complicates the NO detection since esterase activities are affected by cell health and many other factors. 2). DAX-2 product exhibits pH-independent fluorescence while DAF-2 has its fluorescence highly affected by pH. 3). DAX-J2 Red fluorescent product is much more photostable than DAF-2 product. 4). It is more sensitive for detecting NO than DAF-2. 5). It can be used with GFP cell lines or with the applications that use FITC-labeled antibodies for multicolor cell analysis.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DAX-J2™ Red to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM164.266 µL821.328 µL1.643 mL8.213 mL16.427 mL
5 mM32.853 µL164.266 µL328.531 µL1.643 mL3.285 mL
10 mM16.427 µL82.133 µL164.266 µL821.328 µL1.643 mL

Molarity calculator

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Spectral properties

Excitation (nm)587
Emission (nm)609

Product Family

NameExcitation (nm)Emission (nm)
DAX-J2™ Orange552575



View all 7 citations: Citation Explorer
Presynaptic NK1 Receptor Activation by Substance P Suppresses EPSCs via Nitric Oxide Synthesis in the Rat Insular Cortex
Authors: Matsumura, Sachie and Yamamoto, Kiyofumi and Nakaya, Yuka and O'Hashi, Kazunori and Kaneko, Keisuke and Takei, Hiroki and Tsuda, Hiromasa and Shirakawa, Tetsuo and Kobayashi, Masayuki
Journal: Neuroscience (2021): 151--164
One-step preparation of the engineered titanium implant by rationally designed linear fusion peptides with spacer-dependent antimicrobial, anti-inflammatory and osteogenic activities
Authors: Xin, Haoqian and Chen, Junjian and Li, Tianjie and Hu, Guansong and Fang, Zhou and Zhou, Haiyan and Guo, Kunzhong and Wang, Lin and Wang, Yingjun
Journal: Chemical Engineering Journal (2021): 130380
Aspirin eugenol ester attenuates oxidative injury of vascular endothelial cells by regulating NOS and Nrf2 signaling pathways
Authors: Huang, Mei-Zhou and Yang, Ya-Jun and Liu, Xi-Wang and Qin, Zhe and Li, Jian-Yong
Journal: British Journal of Pharmacology (2019)
Fluorescent real-time quantitative measurements of intracellular peroxynitrite generation and inhibition
Authors: Luo, Zhen and Zhao, Qin and Liu, Jixiang and Liao, Jinfang and Peng, Ruogu and Xi, Yunting and Diwu, Zhenjun
Journal: Analytical biochemistry (2017): 44--48
Inducible Nitric Oxide Synthase (iNOS) Is a Novel Negative Regulator of Hematopoietic Stem/Progenitor Cell Trafficking
Authors: Adamiak, Mateusz and Abdelbaset-Ismail, Ahmed and Moore, Joseph B and Zhao, J and Abdel-Latif, Ahmed and Wysoczynski, Marcin and Ratajczak, Mariusz Z
Journal: Stem Cell Reviews and Reports (2016): 1--12


View all 139 references: Citation Explorer
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