Most cellular ROS are generated endogenously as byproducts of mitochondrial oxidative phosphorylation, or formed as intermediates of oxidoreductase enzymes and metal catalyzed oxidation. Since oxygen atoms contain two unpaired electrons in separate orbits of its outer electron shell, it is susceptible to radical formation. The sequential reduction of oxygen through the addition of electrons leads to the formation of a number of ROS including nitric oxide (NO).
DAF-2 reagents are frequently used to detect nitric oxide (NO). However, DAF-2 diacetate is spontaneously hydrolyzed in cell culture media. The hydrolyzed DAF-2 is not cell-permeable, thus causing high assay background. DAX-J2™ probes are developed as excellent replacements for DAF-2 for the detection and bioimaging of NO. Compared to DAF-2 reagents, DAX-J2™ reagents have longer wavelengths and better stability.
The DAX-J2™ series universally demonstrate pH-independent fluorescent response and are highly specific to NO even in the presence of multiple reactive oxygen species
(ROS).
DAX-J2™ series are non-fluorescent reagents that can measure free NO and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the blocking groups on the DAX-J2 reagent are released to generate the highly fluorescent product upon NO oxidation. The majority of the dyes are cell-permeable, with the lone exception,
DAX-J2™ IR, instead being water-soluble. The entire series exhibits pH-independent fluorescence.
Fluorescence Spectrum Viewer
Need assistance selecting the best fluorophore for your experiment, use our Fluorescence Spectrum Viewer:
Other Series Kits Integrating DAX-J2™ Sensors
The
Amplite™ and Cell Meter™ product series both include peroxynitrite kits utilizing the DAX-J2™ PON Green 99 sensor, which specifically reacts with peroxynitrite (ONOO-) with high selectivity over other reactive oxygen species
(ROS) and reactive nitrogen species (RNS) to generate a bright green glow easily measured using the common FITC filter.