Name: DAX-J2™ Red
Brand: AAT Bioquest
SKU: 16301
Price: 174 USD
Availability: InStock

DAX-J2™ Red

DAX-J2™ Red is a new nitric oxide (NO) sensor recently developed by AAT Bioquest. It is a non-fluorescent cell permeable reagent that can measure free NO and nitric oxide synthase (NOS) activity in living cells under physiological conditions. Once inside the cell the blocking groups on the DAX-J2 reagent are released to generate the highly fluorescent product upon NO oxidation. DAX-J2 fluorescent product can be detected using the filter set of Texas Red® that is equipped with most of flow cytometers and fluorescence microscope. DAX-J2 Red has distinct advantages for NO detection than the popular DAF-2 NO probe: 1). It does not require esterase activity for NO detection. DAF-2 requires intracellular esterases to cleave its acetate groups for detecting NO activity. This esterase dependence often complicates the NO detection since esterase activities are affected by cell health and many other factors. 2). DAX-2 product exhibits pH-independent fluorescence while DAF-2 has its fluorescence highly affected by pH. 3). DAX-J2 Red fluorescent product is much more photostable than DAF-2 product. 4). It is more sensitive for detecting NO than DAF-2. 5). It can be used with GFP cell lines or with the applications that use FITC-labeled antibodies for multicolor cell analysis.

Fluorescence response of DAX-J2<sup>TM</sup> Red (2 uM) to different reactive oxygen species (1 mM) in PBS buffer (pH 7.2). The fluorescence intensities were measured with Ex/Em = 570/610 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
16301	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DAX-J2™ Red to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	164.266 µL	821.328 µL	1.643 mL	8.213 mL	16.427 mL
5 mM	32.853 µL	164.266 µL	328.531 µL	1.643 mL	3.285 mL
10 mM	16.427 µL	82.133 µL	164.266 µL	821.328 µL	1.643 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	608.77 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

Synthesis, structure, spectra, cytotoxicity and photo induced NO release of four isomeric nitrosylruthenium complexes

Authors:

Shi, Jia and Liu, Yuhua and Jiao, Shuxiang and Wu, Tao and Wang, Ai and Wang, Wenming and Xie, Leilei and Liu, Yanhong and Wang, Hongfei

Journal:

Nitric Oxide (2024)

Low-dose daylight exposure induces nitric oxide release and maintains cell viability in vitro

Authors:

Hazell, Gareth and Khazova, Marina

Substance P-induced activation of presynaptic NK1 receptors suppresses EPSCs via nitric oxide synthesis in the rat insular cortex

Authors:

松村幸恵,

Presynaptic NK1 Receptor Activation by Substance P Suppresses EPSCs via Nitric Oxide Synthesis in the Rat Insular Cortex

Authors:

Matsumura, Sachie and Yamamoto, Kiyofumi and Nakaya, Yuka and O'Hashi, Kazunori and Kaneko, Keisuke and Takei, Hiroki and Tsuda, Hiromasa and Shirakawa, Tetsuo and Kobayashi, Masayuki

Journal:

Neuroscience (2021): 151--164

One-step preparation of the engineered titanium implant by rationally designed linear fusion peptides with spacer-dependent antimicrobial, anti-inflammatory and osteogenic activities

Authors:

Xin, Haoqian and Chen, Junjian and Li, Tianjie and Hu, Guansong and Fang, Zhou and Zhou, Haiyan and Guo, Kunzhong and Wang, Lin and Wang, Yingjun

Journal:

Chemical Engineering Journal (2021): 130380

References

View all 139 references: Citation Explorer

Pitfalls and limitations in using 4,5-diaminofluorescein for evaluating the influence of polyphenols on nitric oxide release from endothelial cells

Authors:

Uhlenhut K, Hogger P.

Journal:

Free Radic Biol Med (2012): 2266

Effects of moderate electrical stimulation on reactive species production by primary rat skeletal muscle cells: cross talk between superoxide and nitric oxide production

Authors:

Lambertucci RH, Silveira Ldos R, Hirabara SM, Curi R, Sweeney G, Pithon-Curi TC.

Journal:

J Cell Physiol (2012): 2511

Improved measurements of intracellular nitric oxide in intact microvessels using 4,5-diaminofluorescein diacetate

Authors:

Zhou X, He P.

Journal:

Am J Physiol Heart Circ Physiol (2011): H108

Aging negatively affects estrogens-mediated effects on nitric oxide bioavailability by shifting ERalpha/ERbeta balance in female mice

Authors:

Novensa L, Novella S, Medina P, Segarra G, Castillo N, Heras M, Hermenegildo C, Dantas AP.

Journal:

PLoS One (2011): e25335

Temporal and spatial correlation of platelet-activating factor-induced increases in endothelial [Ca(2)(+)]i, nitric oxide, and gap formation in intact venules

Authors:

Zhou X, He P.

Journal:

Am J Physiol Heart Circ Physiol (2011): H1788

Physical properties

Molecular weight	608.77
Solvent	DMSO

Spectral properties

Excitation (nm)	587
Emission (nm)	609

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
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Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 9, 2025