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DiD labeling solution [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine] *5 mM DMSO solution*

Chemical structure for DiD labeling solution [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine] *5 mM DMSO solution*
Chemical structure for DiD labeling solution [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine] *5 mM DMSO solution*
Ordering information
Price ()
Catalog Number22033
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight959.91
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)260000
Excitation (nm)646
Emission (nm)663
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
127274-91-3
Molecular weight
959.91
Extinction coefficient (cm -1 M -1)
260000
Excitation (nm)
646
Emission (nm)
663
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiD labeling solution [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine] *5 mM DMSO solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM104.176 µL520.882 µL1.042 mL5.209 mL10.418 mL
5 mM20.835 µL104.176 µL208.353 µL1.042 mL2.084 mL
10 mM10.418 µL52.088 µL104.176 µL520.882 µL1.042 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)260000
Excitation (nm)646
Emission (nm)663

Citations


View all 55 citations: Citation Explorer
Salivary protein glycopatterns for natural regulation of oral microbiota
Authors: Shu, Jian and Yu, Hanjie and Wang, Yan and Wang, Xilong and Ren, Xiameng and Zhang, Kun and Tang, Zhen and Chen, Wentian and Li, Baozhen and Li, Zheng
Journal: (2021)
A Stable Cell Membrane-Based Coating with Antibiofouling and Macrophage Immunoregulatory Properties for Implants at the Macroscopic Level
Authors: Dong, Zhiyun and Ke, Xiang and Tang, Shuxian and Wu, Shuai and Wu, Wei and Chen, Xingyu and Yang, Jiaojiao and Xie, Jing and Luo, Jun and Li, Jianshu
Journal: Chemistry of Materials (2021)
Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer
Authors: Huang, Yanjuan and Guan, Zilin and Dai, Xiuling and Shen, Yifeng and Wei, Qin and Ren, Lingling and Jiang, Jingwen and Xiao, Zhanghong and Jiang, Yali and Liu, Di and others,
Journal: Nature Communications (2021): 1--22
Chemosensitization of Temozolomide-Resistant Pediatric Diffuse Midline Glioma Using Potent Nanoencapsulated Forms of a N (3)-Propargyl Analogue
Authors: Heravi Shargh, Vahid and Luckett, Jeni and Bouzinab, Kaouthar and Paisey, Stephen and Turyanska, Lyudmila and Singleton, William GB and Lowis, Stephen and Gershkovich, Pavel and Bradshaw, Tracey D and Stevens, Malcolm FG and others,
Journal: ACS Applied Materials \& Interfaces (2021)
Heparanase induces necroptosis of microvascular endothelial cells to promote the metastasis of hepatocellular carcinoma
Authors: Chen, Xiaopeng and Cheng, Bin and Dai, Dafei and Wu, Yuhai and Feng, Zhiwen and Tong, Chaogang and Wang, Xiangming and Zhao, Jun
Journal: Cell death discovery (2021): 1--13
Polymer-loaded hydrogels serve as depots for lactate and mimic “cold” tumor microenvironments
Authors: Allen, Riley and Ivtchenko, Emilie and Thuamsang, Bhasirie and Sangsuwan, Rapeepat and Lewis, Jamal S
Journal: Biomaterials science (2020): 6056--6068
Near-infrared laser-triggered, self-immolative smart polymersomes for in vivo cancer therapy
Authors: Tang, Qing and Hu, Ping and Peng, Haibo and Zhang, Ning and Zheng, Qiang and He, Yun
Journal: International Journal of Nanomedicine (2020): 137
3D super-resolved imaging in live cells using sub-diffractive plasmonic localization of hybrid nanopillar arrays
Authors: Kim, Soojung and Song, Hyerin and Ahn, Heesang and Jun, Seung Won and Kim, Seungchul and Song, Young Min and Yang, Seung Yun and Kim, Chang-Seok and Kim, Kyujung
Journal: Nanophotonics (2020)
The Influence of Cell Source and Donor Age on the Tenogenic Potential and Chemokine Secretion of Human Mesenchymal Stromal Cells
Authors: Zarychta-Wisniewska, Weronika and Burdzińska, Anna and Zielniok, Katarzyna and Koblowska, Marta and Gala, Kamila and Pedzisz, Piotr and Iwanicka-Nowicka, Roksana and Fogtman, Anna and Aksamit, Aleks and ra , undefined and Kulesza, Agnieszka and others, undefined
Journal: Stem Cells International (2019)
Exosomes from macrophages exposed to apoptotic breast cancer cells promote breast cancer proliferation and metastasis
Authors: Yu, Xiuyan and Zhang, Qun and Zhang, Xuehui and Han, Qing and Li, Hui and Mao, Yiqing and Wang, Xi and Guo, Hongyan and Irwin, David M and Niu, Gang and others,
Journal: Journal of Cancer (2019): 2892

References


View all 92 references: Citation Explorer
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
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Journal: Hypertension (2006): 482
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
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Journal: J Biomed Mater Res B Appl Biomater. (2006)
Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing
Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Afferent and efferent connections of the cerebellum of the chondrostean Acipenser baeri: a carbocyanine dye (DiI) tracing study
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Journal: J Comp Neurol (2003): 327
A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle
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Journal: J Electron Microsc (Tokyo) (2003): 327
Retinal ganglion cells resistant to advanced glaucoma: a postmortem study of human retinas with the carbocyanine dye DiI
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice
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Journal: Histochem Cell Biol (2003): 483