Name: DiFMUP
Brand: AAT Bioquest
SKU: 11627
Price: 115 USD
Availability: InStock

DiFMUP

DiFMUP is a fluorinated MUP, which is a commonly used fluorogenic alkaline phosphatase substrate. The reaction product of DiFMUP has excitation/emission maxima of ~358/450 nm, essentially identical to the spectral properties of MUP. Upon phosphatase-induced hydrolysis DiFMUP generates fluorinated 4-methylumbelliferyl fluorophore that has a lower pKa than that of the coumarin fluorophore generated by MUP, making DiFMUP a superior substrate for continuously assaying acid phosphatases at low pH than MUP. DiFMUP is also used for monitoring protein phosphatase activities.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11627	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiFMUP to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	342.313 µL	1.712 mL	3.423 mL	17.116 mL	34.231 mL
5 mM	68.463 µL	342.313 µL	684.627 µL	3.423 mL	6.846 mL
10 mM	34.231 µL	171.157 µL	342.313 µL	1.712 mL	3.423 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	292.13 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

PPP2R5D promotes hepatitis C virus infection by binding to viral NS5B and enhancing viral RNA replication

Authors:

Anwar, Muhammad Ikram and Li, Ni and Zhou, Qing and Chen, Mingxiao and Hu, Chengguang and Wu, Tao and Chen, Haihang and Li, Yi-Ping and Zhou, Yuanping

Journal:

Virology Journal (2022): 1--12

Proteomic analysis of the hepatotoxicity of Microcystis aeruginosa in adult zebrafish (Danio rerio) and its potential mechanisms

Authors:

Du, Benben and Liu, Guangfu and Ke, Mingjing and Zhang, Zhenyan and Zheng, Meng and Lu, Tao and Sun, Liwei and Qian, Haifeng

Journal:

Environmental Pollution (2019): 113019

Spontaneous and chaperone-assisted metal loading in the active site of protein phosphatase-1

Authors:

Van der Hoeven, Gerd and Lemaire, Sarah and Cao, Xinyu and Claes, Zander and Karamanou, Spyridoula and Bollen, Mathieu

Journal:

FEBS letters

Physical properties

Molecular weight	292.13
Solvent	DMSO

Spectral properties

Excitation (nm)	358
Emission (nm)	450

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	214491-43-7

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Page updated on October 8, 2025