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AAT Bioquest


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InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight292.13
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
DiFMUP is a fluorinated MUP, which is a commonly used fluorogenic alkaline phosphatase substrate. The reaction product of DiFMUP has excitation/emission maxima of ~358/450 nm, essentially identical to the spectral properties of MUP. Upon phosphatase-induced hydrolysis DiFMUP generates fluorinated 4-methylumbelliferyl fluorophore that has a lower pKa than that of the coumarin fluorophore generated by MUP, making DiFMUP a superior substrate for continuously assaying acid phosphatases at low pH than MUP. DiFMUP is also used for monitoring protein phosphatase activities.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiFMUP to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM342.313 µL1.712 mL3.423 mL17.116 mL34.231 mL
5 mM68.463 µL342.313 µL684.627 µL3.423 mL6.846 mL
10 mM34.231 µL171.157 µL342.313 µL1.712 mL3.423 mL

Molarity calculator

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Spectral properties



View all 2 citations: Citation Explorer
PPP2R5D promotes hepatitis C virus infection by binding to viral NS5B and enhancing viral RNA replication
Authors: Anwar, Muhammad Ikram and Li, Ni and Zhou, Qing and Chen, Mingxiao and Hu, Chengguang and Wu, Tao and Chen, Haihang and Li, Yi-Ping and Zhou, Yuanping
Journal: Virology Journal (2022): 1--12
Proteomic analysis of the hepatotoxicity of Microcystis aeruginosa in adult zebrafish (Danio rerio) and its potential mechanisms
Authors: Du, Benben and Liu, Guangfu and Ke, Mingjing and Zhang, Zhenyan and Zheng, Meng and Lu, Tao and Sun, Liwei and Qian, Haifeng
Journal: Environmental Pollution (2019): 113019