DiFMUP
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 292.13 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 358 |
Emission (nm) | 450 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Alkaline Phosphatase (ALP), Biotin and Streptavidin, Coumarins, Enzyme-Linked Immunosorbent Assay (ELISA), Secondary Antibodies
CAS 214491-43-7 | Molecular weight 292.13 | Excitation (nm) 358 | Emission (nm) 450 |
DiFMUP is a fluorinated MUP, which is a commonly used fluorogenic alkaline phosphatase substrate. The reaction product of DiFMUP has excitation/emission maxima of ~358/450 nm, essentially identical to the spectral properties of MUP. Upon phosphatase-induced hydrolysis DiFMUP generates fluorinated 4-methylumbelliferyl fluorophore that has a lower pKa than that of the coumarin fluorophore generated by MUP, making DiFMUP a superior substrate for continuously assaying acid phosphatases at low pH than MUP. DiFMUP is also used for monitoring protein phosphatase activities.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiFMUP to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 342.313 µL | 1.712 mL | 3.423 mL | 17.116 mL | 34.231 mL |
5 mM | 68.463 µL | 342.313 µL | 684.627 µL | 3.423 mL | 6.846 mL |
10 mM | 34.231 µL | 171.157 µL | 342.313 µL | 1.712 mL | 3.423 mL |
Molarity calculator
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Citations
View all 2 citations: Citation Explorer
PPP2R5D promotes hepatitis C virus infection by binding to viral NS5B and enhancing viral RNA replication
Authors: Anwar, Muhammad Ikram and Li, Ni and Zhou, Qing and Chen, Mingxiao and Hu, Chengguang and Wu, Tao and Chen, Haihang and Li, Yi-Ping and Zhou, Yuanping
Journal: Virology Journal (2022): 1--12
Authors: Anwar, Muhammad Ikram and Li, Ni and Zhou, Qing and Chen, Mingxiao and Hu, Chengguang and Wu, Tao and Chen, Haihang and Li, Yi-Ping and Zhou, Yuanping
Journal: Virology Journal (2022): 1--12
Proteomic analysis of the hepatotoxicity of Microcystis aeruginosa in adult zebrafish (Danio rerio) and its potential mechanisms
Authors: Du, Benben and Liu, Guangfu and Ke, Mingjing and Zhang, Zhenyan and Zheng, Meng and Lu, Tao and Sun, Liwei and Qian, Haifeng
Journal: Environmental Pollution (2019): 113019
Authors: Du, Benben and Liu, Guangfu and Ke, Mingjing and Zhang, Zhenyan and Zheng, Meng and Lu, Tao and Sun, Liwei and Qian, Haifeng
Journal: Environmental Pollution (2019): 113019
Application notes
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A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
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