AAT Bioquest

FastClick™ 6-FAM Alkyne

The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne does not effectively react with the coumarin azide substrate.
The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne does not effectively react with the coumarin azide substrate.
The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne does not effectively react with the coumarin azide substrate.
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Physical properties
Molecular weight684.75
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
FastClick™ 6-FAM Azide
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See also: Click Chemistry
Molecular weight
FastClick™ 6-FAM Alkyne contains both the moiety of FastClick (for assisting click efficiency) and 6-FAM fluorophore (as the fluorescence tag) for developing 6-FAM-based fluorescent probes. It readily reacts with an azido-modified biomolecule under extremely mild conditions. 6-FAM is one of the most common green fluorophores used for labeling biomolecules, in particular, for labeling oligonucleotides. 6-FAM conjugates are widely used in fluorescence-based nucleic acid detections such PCR and FISH etc. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ 6-FAM Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM146.039 µL730.194 µL1.46 mL7.302 mL14.604 mL
5 mM29.208 µL146.039 µL292.077 µL1.46 mL2.921 mL
10 mM14.604 µL73.019 µL146.039 µL730.194 µL1.46 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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View all 50 references: Citation Explorer
Programmed immobilization of living cells using independent click pairs.
Authors: Zhu, Chengyuan and Takemoto, Hiroyasu and Higuchi, Yuriko and Yamashita, Fumiyoshi
Journal: Biochemical and biophysical research communications (2024): 149556
Visualizable and Lubricating Hydrogel Microspheres Via NanoPOSS for Cartilage Regeneration.
Authors: Yao, Yubin and Wei, Gang and Deng, Lianfu and Cui, Wenguo
Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2023): e2207438
Ultrasensitive detection of miRNA-21 by click chemistry and fluorescein-mediated photo-ATRP signal amplification.
Authors: Yu, Shuaibing and Zhang, Jian and Hu, Yaodong and Li, Lianzhi and Kong, Jinming and Zhang, Xueji
Journal: Analytica chimica acta (2023): 341661
Targeted delivery of nuclear targeting probe for bladder cancer using cyclic pentapeptide c(RGDfK) and acridine orange.
Authors: Qin, Jiaxin and Liang, Qing and Wang, Guangyue and Hao, Lin and Liu, Xing and Wang, Xinlei and Hu, Zhengxiang and Fang, Gaochuan and Xue, Liang and Zhao, Yan and Li, Rui and Lv, Qian and Wen, Jiling and Yang, Guosheng and Han, Conghui and Shi, Zhenduo
Journal: Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Can (2023): 375-383
Divergent Synthesis of Ultrabright and Dendritic Xanthenes for Enhanced Click-Chemistry-Based Bioimaging.
Authors: Montiel, Luis and Spada, Fabio and Crisp, Antony and Serdjukow, Sascha and Carell, Thomas and Frischmuth, Thomas
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202202633
Asymmetric rotaxanes as dual-modality supramolecular imaging agents for targeting cancer biomarkers.
Authors: d'Orchymont, Faustine and Holland, Jason P
Journal: Communications chemistry (2023): 107
In-Line Characterization of the Temperature-Responsive Behavior of Surface-Bound Microgel Coatings by QCM-D: A Novel Strategy for Protein Repellence Evaluation.
Authors: Santi, Marta and Saha, Pabitra and Walkowiak, Jacek Janusz and Rubner, Jens and Wessling, Matthias and Pich, Andrij
Journal: ACS applied materials & interfaces (2022): 10907-10916
Facile fabrication of semi-IPN hydrogel adsorbent based on quaternary cellulose via amino-anhydride click reaction in water.
Authors: Liu, Yuhua and Wei, Hongliang and Li, Songmao and Wang, Gang and Guo, Tao and Han, Huayun
Journal: International journal of biological macromolecules (2022): 622-634
Layer-by-Layer Surface Modification of Alendronate-Loaded Polyester Microparticles-Enabling Protein Immobilization.
Authors: Urbaniak, Tomasz and Musiał, Witold
Journal: Polymers (2022)
"Fix and Click" for Assay of Sphingolipid Signaling in Single Primary Human Intestinal Epithelial Cells.
Authors: Gallion, Luke A and Wang, Yuli and Massaro, Angelo and Yao, Ming and Petersen, Brae V and Zhang, Quanzheng and Huang, Weigang and Carr, Adam J and Zhang, Qisheng and Allbritton, Nancy L
Journal: Analytical chemistry (2022): 1594-1600