Name: FITC Anti-human CD135 Antibody *BV10A4*
Brand: AAT Bioquest
SKU: 113501G0
Availability: InStock

FITC Anti-human CD135 Antibody

BV10A4

BV10A4 is an anti-human monoclonal antibody that forms an immune complex with the CD135 antigen. CD135 (also known as FLT3, FLK2 or STK-1) is a 130 - 160 kD member of the immunoglobulin supergene family that is located on the surface of cells like stem cells. CD135 acts in important cellular pathways, in particular, the transmembrane receptor protein tyrosine kinase signaling pathway and cytokine-mediated signaling pathway. Also, in certain organisms, it positively regulates tyrosine phosphorylation of STAT protein, positively regulates phosphatidylinositol 3-kinase activity and is a promoter of phosphatidylinositol 3-kinase signaling. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like FL (flt3 ligand). CD135 is a relatively rare antibody target, with fewer than 900 publications in the last decade. Even still, CD135 has a variety of applications in immunology and cell biology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to FITC (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 525/50 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant X).

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
113501G0	100 tests	Price
113501G1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD135 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	FLT3, FLK2, STK-1
Clone	BV10A4
Host	Mouse
Isotype	IgG1
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	488
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on July 22, 2026