Name: FITC Anti-human CD235 Antibody *HIR2*
Brand: AAT Bioquest
SKU: 123511I0
Availability: InStock

FITC Anti-human CD235 Antibody

HIR2

HIR2 is an anti-human monoclonal antibody that targets the CD235a antigen. CD235a (also known as Glycophorin A) is a 10 kD single-pass type i membrane protein that is found on the surface of cells such as stem cells and erythrocytes. CD235 is associated with a variety of biologically interesting macromolecules/ligands, in particular, Plasmodium falci- parum erythrocyte binding antigen EBA-175. CD235 is a relatively rare antibody target, with less than 0 publications in the last decade. Even still, CD235a has been widely used in cell adhesion and immunology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to FITC (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 528/65 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).

Protocol

COA

Catalog	Size	Price	Quantity
123511I0	100 tests	Price
123511I1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd235a antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	GYPA; Sialoglycoprotein alpha; PAS-2
Clone	HIR2
Host	mouse
Isotype	mouse IgG2b
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 25, 2025