Name: FITC Anti-human CD28 Antibody *9.3*
Brand: AAT Bioquest
SKU: 102811I0
Availability: InStock

FITC Anti-human CD28 Antibody

9.3

The 9.3 monoclonal antibody binds with human CD28, a 44 kD transmembrane glycoprotein typically located on the surface of natural killer cells and plasma cells. CD28 is a member of vital cellular pathways, namely, the cell surface receptor signaling pathway, apoptotic signaling pathway and T cell receptor signaling pathway. Also, in some organisms, it is involved in the positive regulation of interleukin-4 production, is involved in the positive regulation of inflammatory response to antigenic stimulus and promotes isotype switching to IgG isotypes. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as PI3-kinase, CD86 and CD80. CD28 is a very popular antibody target, with over 30000 publications in the last decade. CD28 is vital to costimulatory molecules research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to FITC (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 525/50 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant Analyzer 16).

Datasheet

COA

Catalog	Size	Price	Quantity
102811I0	100 tests	Price
102811I1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD28 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Tp44, T44
Clone	9.3
Host	Mouse
Isotype	IgG2a
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	488
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on August 21, 2026