Name: FITC Anti-human CD4 Antibody *OKT-4*
Brand: AAT Bioquest
SKU: 100431H0
Availability: InStock

FITC Anti-human CD4 Antibody

OKT-4

OKT-4 is an anti-human monoclonal antibody that targets the CD4 antigen. CD4 (also known as T4) is a 55 kD member of the Ig superfamily that is located on the surface of cells such as macrophages. In certain organisms, CD4 positively regulates kinase activity, promotes I-kappaB kinase/NF-kappaB signaling and upregulates transcription, DNA-templated. Additionally, it has been thought to be involved with essential biological processes such as immune response, especially adaptive immune response. CD4 is a member of critical cellular pathways, for instance, the cytokine-mediated signaling pathway, enzyme linked receptor protein signaling pathway and interleukin-15-mediated signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like Lck and IL-16. CD4 is a very popular antibody target, with over 185000 publications in the last decade. CD4 is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to FITC (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 525/50 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
100431H0	100 tests	Price
100431H1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd4 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Leu-3; T4
Clone	OKT-4
Host	mouse
Isotype	mouse igg2b, κ
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 30, 2025