FITC Anti-human/ rabbit/ cat/ non-human primates/ ferret CD271 Antibody *NGFR5*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.92 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names p75NTR; TNFRSF16; NGFR; Gp80-LNGFR | Clone NGFR5 | Host mouse | Isotype Mouse IgG1 | Reactivity human; rabbit; cat; non-human primates; ferret | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.92 |
NGFR5 is an anti-human/ rabbit/ cat/ non-human primates/ ferret monoclonal antibody that is specific for the CD271 antigen. CD271 (sometimes referred to as TNFRSF16 or Gp80-LNGFR) is a 75 kD transmembrane protein that is found on the surface of cells like dendritic cells. CD271 is a component of important cellular pathways, namely, the neurotrophin TRK receptor signaling pathway, negative regulation of fibroblast growth factor receptor signaling pathway and positive regulation of apoptotic signaling pathway. Also, it has been associated with critical biological processes like glucose homeostasis, especially cellular glucose homeostasis. In certain organisms, CD271 plays a role in the upregulation of pri-miRNA transcription by RNA polymerase II, positively regulates apoptotic process and is a negative regulator of hair follicle development. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like NT-3 and NT-4. CD271 is a fairly uncommon antibody target, with a little more than 1700 publications in the last decade. Even still, CD271 is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of stem cells, immunology and neuroscience. This antibody was purified through affinity chromatography and conjugated to FITC (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 528/46 nm bandpass filter (for example, as in the Luminex Amnis CellStream).
Spectrum
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Spectral properties
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.92 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd271 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?