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Fluo-8L™, potassium salt

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Physical properties
Dissociation constant (Kd, nM)1900
Molecular weight909.09
SolventWater
Spectral properties
Correction Factor (260 nm)1.076
Correction Factor (280 nm)0.769
Extinction coefficient (cm -1 M -1)23430
Excitation (nm)495
Emission (nm)516
Quantum yield0.161
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Show More (78)

OverviewpdfSDSpdfProtocol


See also: Fluoresceins
Molecular weight
909.09
Dissociation constant (Kd, nM)
1900
Correction Factor (260 nm)
1.076
Correction Factor (280 nm)
0.769
Extinction coefficient (cm -1 M -1)
23430
Excitation (nm)
495
Emission (nm)
516
Quantum yield
0.161
Calcium measurements are critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Fluo-3 AM and Fluo-4 AM are most commonly used among the visible light-excitable calcium indicators for live-cell calcium imaging. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8® dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelengths of Ex/Em = ∼490/∼520 nm. Fluo-8® AM can be loaded into cells at room temperature, while Fluo-3 AM and Fluo-4 AM require 37°C for cell loading. In addition, Fluo-8® AM is two times brighter than Fluo-4 AM and four times brighter than Fluo-3 AM. AAT Bioquest offers a set of our outstanding Fluo-8® reagents with different calcium-binding affinities (Fluo-8® Kd = 389 nM; Fluo-8H™ Kd = 232 nM; Fluo-8L™ Kd = 1.86 µM; Fluo-8FF™ Kd = 10 µM). We also offer versatile packing sizes to meet your special needs (e.g., 1 mg, 10x50 µg, 20x50 µg, and HTS packages) with no additional packaging charge.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluo-8L™, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM110 µL550.001 µL1.1 mL5.5 mL11 mL
5 mM22 µL110 µL220 µL1.1 mL2.2 mL
10 mM11 µL55 µL110 µL550.001 µL1.1 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)1.076
Correction Factor (280 nm)0.769
Extinction coefficient (cm -1 M -1)23430
Excitation (nm)495
Emission (nm)516
Quantum yield0.161

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Fluo-8®, potassium salt495516234300.1611.0760.769
Fluo-8L™, sodium salt 495516234300.1611.0760.769
Fluo-8FF™, potassium salt 495516234300.1611.0760.769

Citations


View all 153 citations: Citation Explorer
Ex vivo replication of phenotypic functions of osteocytes through biomimetic 3D bone tissue construction
Authors: Sun, Qiaoling and Choudhary, Saba and Mannion, Ciaran and Kissin, Yair and Zilberberg, Jenny and Lee, Woo Y
Journal: Bone (2017)
Non-steroidal anti-inflammatory drug delays corneal wound healing by reducing production of 12-hydroxyheptadecatrienoic acid, a ligand for leukotriene B4 receptor 2
Authors: Iwamoto, Satoshi and Koga, Tomoaki and Ohba, Mai and Okuno, Toshiaki and Koike, Masato and Murakami, Akira and Matsuda, Akira and Yokomizo, Takehiko
Journal: Scientific Reports (2017)
Bystander effects elicited by single-cell photo-oxidative blue-light stimulation in retinal pigment epithelium cell networks
Authors: Ishii, Masaaki and Rohrer, B&auml;rbel
Journal: Cell Death Discovery (2017): 16071
High Glucose Enhances Isoflurane-Induced Neurotoxicity by Regulating TRPC-Dependent Calcium Influx
Authors: Liu, ZhongJie and Ma, ChangQing and Zhao, Wei and Zhang, QingGuo and Xu, Rui and Zhang, HongFei and Lei, HongYi and Xu, ShiYuan
Journal: Neurochemical Research (2017): 1--14
Hyperforin/HP-&beta;-Cyclodextrin Enhances Mechanosensitive Ca2
Authors: Takada, Hiroya and Yonekawa, Jun and Matsumoto, Masami and Furuya, Kishio and Sokabe, Masahiro
Journal: BioMed Research International (2017)
High-throughput screen detects calcium signaling dysfunction in typical sporadic autism spectrum disorder
Authors: Schmunk, Galina and Nguyen, Rachel L and Ferguson, David L and Kumar, Kenny and Parker, Ian and Gargus, J Jay
Journal: Scientific Reports (2017): 40740
Aryl-and alkyl-phosphorus-containing flame retardants induced mitochondrial impairment and cell death in Chinese hamster ovary (CHO-k1) cells
Authors: Huang, Chao and Li, Na and Yuan, Shengwu and Ji, Xiaoya and Ma, Mei and Rao, Kaifeng and Wang, Zijian
Journal: Environmental Pollution (2017): 775--786
Protective effect of quercetin on bupivacaine-induced neurotoxicity via T-type calcium channel inhibition
Authors: Jin, Chao and Wu, Huisheng and Tang, Chaoliang and Ke, Jianjuan and Wang, Yanlin
Journal: Tropical Journal of Pharmaceutical Research (2017): 1827--1833
The establishment of appropriate methods for egg-activation by human PLCZ1 RNA injection into human oocyte
Authors: Yamaguchi, Takashi and Ito, Masahiko and Kuroda, Keiji and Takeda, Satoru and Tanaka, Atsushi
Journal: Cell Calcium (2017)