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Fluorescein dicaprylate [Fluorescein dioctanoate]

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Physical properties
Molecular weight584.70
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Fluoresceins
Molecular weight
Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
0.79001, 0.952
Fluorescein dicaprylate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the dicaprylate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. Fluorescein dicaprylate may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. Fluorescein dicaprylate may stain Salmonella in green fluorescence. It is a fluorogenic substrate for C8 esterase that generates green fluorescence upon esterase cleavage.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein dicaprylate [Fluorescein dioctanoate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM171.028 µL855.139 µL1.71 mL8.551 mL17.103 mL
5 mM34.206 µL171.028 µL342.056 µL1.71 mL3.421 mL
10 mM17.103 µL85.514 µL171.028 µL855.139 µL1.71 mL

Molarity calculator

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Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Fluorescein biotin4985178000010.79001, 0.9520.320.35
Fluorescein DHPE4985178000010.79001, 0.9520.320.35
Fluorescein aldehyde [5-FAM aldehyde]49351783000-0.320.178
Fluorescein Tyramide4985178000010.79001, 0.9520.320.35



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Identification of VAR2CSA domain-specific inhibitory antibodies of the Plasmodium falciparum erythrocyte membrane protein 1 using a novel flow cytometry assay
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View all 68 references: Citation Explorer
Novel method for cell debris removal in the flow cytometric cell cycle analysis using carboxy-fluorescein diacetate succinimidyl ester
Authors: Terho P, Lassila O.
Journal: Cytometry A (2006): 552
Modification of the fluorescein diacetate assay for screening of antifungal agents against Candida albicans: comparison with the NCCLS methods
Authors: Brouwer N, Kohen J, Jamie J, Vemulpad S.
Journal: J Microbiol Methods (2006): 234
Optimisation of the fluorescein diacetate antibacterial assay
Authors: Wan, undefined and y S, Brouwer N, Liu Q, Mahon A, Cork S, Karuso P, Vemulpad S, Jamie J.
Journal: J Microbiol Methods (2005): 21
A three-dimensional flow control concept for single-cell experiments on a microchip. 2. Fluorescein diacetate metabolism and calcium mobilization in a single yeast cell as stimulated by glucose and pH changes
Authors: Peng XY, Li PC.
Journal: Anal Chem (2004): 5282
Effect of immunosuppressants on T-cell subsets observed in vivo using carboxy-fluorescein diacetate succinimidyl ester labeling
Authors: Hu H, Dong Y, Feng P, Fechner J, Hamawy M, Knechtle SJ.
Journal: Transplantation (2003): 1075
Carboxy-fluorescein diacetate, succinimidyl ester labeled papillomavirus virus-like particles fluoresce after internalization and interact with heparan sulfate for binding and entry
Authors: Drobni P, Mistry N, McMillan N, Ev and er M., undefined
Journal: Virology (2003): 163
Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate
Authors: Lomolino G, Lante A, Crapisi A, Spettoli P, Curioni A.
Journal: Electrophoresis (2001): 1021
Fluorescein diacetate hydrolysis as a measure of fungal biomass in soil
Authors: Gaspar ML, Cabello MN, Pollero R, Aon MA.
Journal: Curr Microbiol (2001): 339
Extrusion of fluorescein diacetate by multidrug-resistant Candida albicans
Authors: Yang HC, Mikami Y, Imai T, Taguchi H, Nishimura K, Miyaji M, Branchini ML.
Journal: Mycoses (2001): 368
Potential problems with fluorescein diacetate assays of cell viability when testing natural products for antimicrobial activity
Authors: Clarke JM, Gillings MR, Altavilla N, Beattie AJ.
Journal: J Microbiol Methods (2001): 261