HIS Lite™ Cy3 Bis NTA Chelator
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1177.30 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.07 |
Correction Factor (280 nm) | 0.073 |
Extinction coefficient (cm -1 M -1) | 1500001 |
Excitation (nm) | 555 |
Emission (nm) | 569 |
Quantum yield | 0.151 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | SDSProtocol |
Molecular weight 1177.30 | Correction Factor (260 nm) 0.07 | Correction Factor (280 nm) 0.073 | Extinction coefficient (cm -1 M -1) 1500001 | Excitation (nm) 555 | Emission (nm) 569 | Quantum yield 0.151 |
Cy3 Bis NTA-Ni Chelator can be applied either to an SDS-PAGE gel or Western blot membrane for fluorescence imaging or used in living cells in combination with the addition of a heavy metal ion such as Ni2+. Detection with Cy3 Bis NTA-Ni complex requires less incubation time than for protein-antibody binding. No secondary reaction is required since Ni-NTA complex is directly conjugated to the Cy3 fluorophores. Cy3 and Cy5 dyes demonstrate strong fluorescent signals at commonly available wavelengths and with little quenching. Polyhistidine is one of the most popular affinity tags incorporated into recombinant proteins. It can be inserted either at the N- or C-terminus and expressed in a variety of hosts. Due to its small size, the polyhistidine tag serves as an elegant tool for both protein purification and detection. AAT Bioquest also offers HIS Lite™ Cy3 Bis NTA-Ni and Cy5 Bis NTA-Ni Complexes that can be directly used for the specific and highly sensitive detection of His-tagged fusion proteins without the addition of a heavy metal ion.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of HIS Lite™ Cy3 Bis NTA Chelator to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 84.94 µL | 424.701 µL | 849.401 µL | 4.247 mL | 8.494 mL |
5 mM | 16.988 µL | 84.94 µL | 169.88 µL | 849.401 µL | 1.699 mL |
10 mM | 8.494 µL | 42.47 µL | 84.94 µL | 424.701 µL | 849.401 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.07 |
Correction Factor (280 nm) | 0.073 |
Extinction coefficient (cm -1 M -1) | 1500001 |
Excitation (nm) | 555 |
Emission (nm) | 569 |
Quantum yield | 0.151 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) |
HIS Lite™ Cy5 Bis NTA Chelator | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
HIS Lite™ Cy3 Tris NTA Chelator | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 | - | - |
Images
References
View all 11 references: Citation Explorer
Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27.
Authors: Coulonval, Katia and Vercruysse, Vincent and Paternot, Sabine and Pita, Jaime M and Corman, Robert and Raspé, Eric and Roger, Pierre P
Journal: Cell cycle (Georgetown, Tex.) (2022): 12-32
Authors: Coulonval, Katia and Vercruysse, Vincent and Paternot, Sabine and Pita, Jaime M and Corman, Robert and Raspé, Eric and Roger, Pierre P
Journal: Cell cycle (Georgetown, Tex.) (2022): 12-32
Selective Acylation of Proteins at Gly and Lys in His Tags.
Authors: Jensen, Knud J and Thygesen, Mikkel B and Sørensen, Kasper K and Wu, Shunliang and Treiberg, Tuule and Schoffelen, Sanne
Journal: Chembiochem : a European journal of chemical biology (2022): e202200359
Authors: Jensen, Knud J and Thygesen, Mikkel B and Sørensen, Kasper K and Wu, Shunliang and Treiberg, Tuule and Schoffelen, Sanne
Journal: Chembiochem : a European journal of chemical biology (2022): e202200359
Controlled delivery of recombinant adeno-associated virus serotype 2 using pH-sensitive poly(ethylene glycol)-poly-L-histidine hydrogels.
Authors: Zeng, Yi-Fang and Tseng, S-Ja and Kempson, Ivan M and Peng, Shu-Fen and Wu, Wen-Teng and Liu, Je-Ruei
Journal: Biomaterials (2012): 9239-45
Authors: Zeng, Yi-Fang and Tseng, S-Ja and Kempson, Ivan M and Peng, Shu-Fen and Wu, Wen-Teng and Liu, Je-Ruei
Journal: Biomaterials (2012): 9239-45
Tumor pH-responsive flower-like micelles of poly(L-lactic acid)-b-poly(ethylene glycol)-b-poly(L-histidine).
Authors: Lee, Eun Seong and Oh, Kyung Taek and Kim, Dongin and Youn, Yu Seok and Bae, You Han
Journal: Journal of controlled release : official journal of the Controlled Release Society (2007): 19-26
Authors: Lee, Eun Seong and Oh, Kyung Taek and Kim, Dongin and Youn, Yu Seok and Bae, You Han
Journal: Journal of controlled release : official journal of the Controlled Release Society (2007): 19-26
Poly(L-histidine)-PEG block copolymer micelles and pH-induced destabilization.
Authors: Lee, Eun Seong and Shin, Hyun Joon and Na, Kun and Bae, You Han
Journal: Journal of controlled release : official journal of the Controlled Release Society (2003): 363-74
Authors: Lee, Eun Seong and Shin, Hyun Joon and Na, Kun and Bae, You Han
Journal: Journal of controlled release : official journal of the Controlled Release Society (2003): 363-74
Non-oxidative modification of lens crystallins by kynurenine: a novel post-translational protein modification with possible relevance to ageing and cataract.
Authors: Garner, B and Shaw, D C and Lindner, R A and Carver, J A and Truscott, R J
Journal: Biochimica et biophysica acta (2000): 265-78
Authors: Garner, B and Shaw, D C and Lindner, R A and Carver, J A and Truscott, R J
Journal: Biochimica et biophysica acta (2000): 265-78
The human cytotoxic T cell granule serine protease granzyme H has chymotrypsin-like (chymase) activity and is taken up into cytoplasmic vesicles reminiscent of granzyme B-containing endosomes.
Authors: Edwards, K M and Kam, C M and Powers, J C and Trapani, J A
Journal: The Journal of biological chemistry (1999): 30468-73
Authors: Edwards, K M and Kam, C M and Powers, J C and Trapani, J A
Journal: The Journal of biological chemistry (1999): 30468-73
Recombinant chicken IFN-gamma expressed in Escherichia coli: analysis of C-terminal truncation and effect on biologic activity.
Authors: Michalski, W P and Shiell, B J and O'Neil, T E and Beddome, G and Lowenthal, J W
Journal: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Resear (1999): 383-92
Authors: Michalski, W P and Shiell, B J and O'Neil, T E and Beddome, G and Lowenthal, J W
Journal: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Resear (1999): 383-92
Expression and characterization of a novel human sperm membrane protein.
Authors: Liu, Q Y and Wang, L F and Miao, S Y and Catterall, J F
Journal: Biology of reproduction (1996): 323-30
Authors: Liu, Q Y and Wang, L F and Miao, S Y and Catterall, J F
Journal: Biology of reproduction (1996): 323-30
Fluorescence signaling of ligand binding and assembly in metal-chelating lipid membranes.
Authors: Maloney, K and Shnek, D and Sasaki, D and Arnold, F
Journal: Chemistry & biology (1996): 185-92
Authors: Maloney, K and Shnek, D and Sasaki, D and Arnold, F
Journal: Chemistry & biology (1996): 185-92