HIS Lite™ Cy3 Bis NTA-Ni Complex
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | 1384.66 |
Solvent | Water |
Correction Factor (260 nm) | 0.07 |
Correction Factor (280 nm) | 0.073 |
Extinction coefficient (cm -1 M -1) | 1500001 |
Excitation (nm) | 555 |
Emission (nm) | 569 |
Quantum yield | 0.151 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
Molecular weight 1384.66 | Correction Factor (260 nm) 0.07 | Correction Factor (280 nm) 0.073 | Extinction coefficient (cm -1 M -1) 1500001 | Excitation (nm) 555 | Emission (nm) 569 | Quantum yield 0.151 |
Platform
Gel Imager
Excitation | Green laser |
Emission | 602/50 nm |
Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Prepare a 5 to 10 mM stock solution by adding an appropriate amount of DMSO.
Note: Store any unused stock solution at -20 °C. Avoid repeated freeze-thaw cycles and minimize light exposure.
PREPARATION OF WORKING SOLUTION
Prepare a 1 to 10 µM HIS Lite™ Cy3 Bis NTA-Ni Complex working solution in PBS.
Note: Ensure that there is sufficient working solution to fully submerge the gel. After use, discard the working solution. Do not reuse.
SAMPLE EXPERIMENTAL PROTOCOL
The following protocol should be used only as a guideline and may require optimization to better suit your specific experimental needs.
Run gels based on your standard protocol.
Place the gel in a suitable container. Fix the gel in the fixing solution for 60 minutes. Note: 40% ethanol + 10% acetic acid can be used as a fixing solution.
Wash the gel twice with the ultra-pure water.
Incubate the gel in the HIS Lite™ Cy3 Bis NTA-Ni Complex working solution for 60 minutes.
Note: Be sure to fully submerge the gel in the working solution.
Remove the working solution and wash the gel twice with PBS.
Proceed to imaging the gel immediately.
Mix the His-tagged protein solution and the HIS Lite™ Cy3 Bis NTA-Ni Complex working solution at the appropriate concentrations.
Note: Optimization of the HIS Lite™ Cy3 Bis NTA-Ni Complex to the His-tagged protein mix must be performed for better labeling.
Note: 1 to 10 µM of HIS Lite™ Cy3 Bis NTA-Ni Complex can be used as a starting concentration.
Note: The reaction can be performed in a buffer containing 50 mM HEPES/KOH, pH 7.4, 100 mM KCl, 1 mM MgCl2, 2 mM β-mercaptoethanol, 5% glycerol, or a buffer of your choice.
Mix can be incubated for 30 minutes at room temperature or 4 ℃.
Note: Optimization of the incubation time and conditions must be performed for better labeling
Mix can then be subjected to column purification or any other downstream process.
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 72.22 µL | 361.099 µL | 722.199 µL | 3.611 mL | 7.222 mL |
5 mM | 14.444 µL | 72.22 µL | 144.44 µL | 722.199 µL | 1.444 mL |
10 mM | 7.222 µL | 36.11 µL | 72.22 µL | 361.099 µL | 722.199 µL |
Molarity calculator
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Spectral properties
Correction Factor (260 nm) | 0.07 |
Correction Factor (280 nm) | 0.073 |
Extinction coefficient (cm -1 M -1) | 1500001 |
Excitation (nm) | 555 |
Emission (nm) | 569 |
Quantum yield | 0.151 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) |
HIS Lite™ Cy5 Bis NTA-Ni Complex | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
HIS Lite™ Cy3 Tris NTA-Ni Complex | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 | - | - |
Citations
Authors: Ou, Ben S and Baillet, Julie and Picece, Vittoria CTM and Gale, Emily C and Powell, Abigail E and Saouaf, Olivia M and Yan, Jerry and Nejatfard, Anahita and Lopez Hernandez, Hector and Appel, Eric A
Journal: ACS nano (2024)
Authors: Ou, Ben S and Picece, Vittoria CTM and Baillet, Julie and Gale, Emily C and Powell, Abigail E and Saouaf, Olivia M and Yan, Jerry and Hernandez, Hector Lopez and Appel, Eric
Journal: bioRxiv (2023)
Authors: Hiranyakorn, Methanee and Yagi-Utsumi, Maho and Yanaka, Saeko and Ohtsuka, Naoya and Momiyama, Norie and Satoh, Tadashi and Kato, Koichi
Journal: International Journal of Molecular Sciences (2023): 6075
Authors: Mo, Weiliang and Zhang, Junchuan and Zhang, Li and Yang, Zhenming and Yang, Liang and Yao, Nan and Xiao, Yong and Li, Tianhong and Li, Yaxing and Zhang, Guangmei and others,
Journal: Nature communications (2022): 1--15
Authors: Roth, Gillie A and Saouaf, Olivia M and Smith, Anton AA and Gale, Emily C and Hernandez, Marcela Alcantara and Idoyaga, Juliana and Appel, Eric A
Journal: ACS biomaterials science \& engineering (2021): 1889--1899
Authors: Saouaf, Olivia M and Roth, Gillie A and Ou, Ben S and Smith, Anton AA and Yu, Anthony C and Gale, Emily C and Grosskopf, Abigail K and Picece, Vittoria CTM and Appel, Eric A
Journal: Journal of Biomedical Materials Research Part A (2021)
References
Authors: de Costa F, Barber CJ, Pujara PT, Reed DW, Covello PS.
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Journal: J Control Release (2015): 115
Authors: Kamkaew M, Chimnaronk S.
Journal: Protein Expr Purif (2015): 43
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Journal: Appl Environ Microbiol (2015): 3243
Authors: Panek A, Pietrow O, Filipkowski P, Synowiecki J.
Journal: Acta Biochim Pol (2013): 163
Authors: Bazin D, Chevalier S, Saadaoui H, Santarelli X, Larpent C, Feracci H, Faure C.
Journal: Langmuir (2012): 13968
Authors: Pinkerneil P, Guldenhaupt J, Gerwert K, Kotting C.
Journal: Chemphyschem (2012): 2649
Authors: Dan H, Balach and ran A, Lin M.
Journal: J Biomol Tech (2009): 241
Authors: Goodman RP, Erben CM, Malo J, Ho WM, McKee ML, Kapanidis AN, Turberfield AJ.
Journal: Chembiochem (2009): 1551
Authors: Tsuji S, Tanaka T, Hirabayashi N, Kato S, Akitomi J, Egashira H, Waga I, Ohtsu T.
Journal: Biochem Biophys Res Commun (2009): 227
Application notes
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