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HRP-labeled goat anti-mouse IgG (H+L)
The enzyme labels can be visualized by means of enzyme histochemical methods via chromogenic (producing color) reactions, or via fluorogenic (producing fluorescence) reactions. The most used enzyme is horse radish peroxidase (HRP). HRP can be visualized by chromogenic reactions such as diaminobenzidine (e.g., DAB), fluorescence (e.g., ADHP) or chemiluminescence (e.g., luminol). The highly purified and cross-absorbed purified IgGs are conjugated to HRP in high yield under a neutral condition to maximally retain the activities of both IgG and HRP using our proprietary Buccutite™ protein crosslinking technology.
Immunofluorescence analysis of Epithelial Cell Adhesion Molecule (EpCAM) expression in lung adenocarcinoma using an anti-EpCAM antibody, followed by HRP-labeled goat anti-mouse IgG secondary antibody (Cat. #16728). Signal amplification was achieved with iFluor® 555 PSA (Cat. #45270). DAPI (blue) was used as a nuclear counterstain.
Immunofluorescence analysis of Epithelial Cell Adhesion Molecule (EpCAM) expression in lung adenocarcinoma using an anti-EpCAM antibody, followed by HRP-labeled goat anti-mouse IgG secondary antibody (Cat. #16728). Signal amplification was achieved with iFluor® 555 PSA (Cat. #45270). DAPI (blue) was used as a nuclear counterstain.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
167281 mg
Price
 
Physical properties
Molecular weight~150000
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025